Die Anwendungsbeispiele der Zickzack-rückungs-methode

In diesem Vortrage diskurrierten wir iiber praktische Beispiele, die wir ein Kleinseilkranksystem, sogenannte Zickzack-riickungs-methode, auf die Durchforstung im Chamaecyparis- wald anzuwenden versuchten. Zuriickschauend auf das Resultat, untersuchten wir im einzelnen die Arbeiten und Zeuge in Durchforstung. Ob es gleich noch der Raum zur Verbesserung gebe, wir urteilten diese Methode als giiltige Durchforstungs-methode im Chamaecypariswald.Article信州大学農学部演習林報告 10: 37-62(1973)departmental bulletin pape

Observation of the Color-Suppressed Decay B̅ 0→D0π0

journal articl

カンヨウケイ コツズイ カンサイボウ ト ジンコウ ザイリョウ オ モチイル ジンコウコツ ソシキ ケイセイ

video/mp4講演場所: バイオサイエンス研究科大講義室講演者所属: 産業技術総合研究所ティッシュエンジニア研究センターvide

日本におけるペットロス研究の動向と展望

departmental bulletin pape

C3d conjugation augments the formation of germinal centers and plasma cells.

<p>A/J mice were analyzed at the indicated time points post-immunization with PA-Av-Alum or PA-Av-C3d₃/IFA. Flow cytometry profiles indicate (A) percent splenic germinal center B cells (CD3⁻, CD11b⁻, IgD⁻, B220⁺, GL7⁺, Fas⁺) of total B cells; (B) percent plasma cells (CD11b⁻, CD3⁻, IgD⁻, B220^lo, Syn1^hi) of IgD⁻ B cells, and (C) percent activated T cells (B220⁻, CD11b⁻, CD4⁺, CD62L^lo, ICOS⁺) of CD4⁺ cells. Representative FACS plots are shown with average and standard deviation (n = 3) of gated population indicated. ELISAs were performed to measure PA-specific (D) IgM and (E) IgG and domain 4-specific IgM (F) and IgG (G) from serum of mice immunized with PA-Av-Alum (white columns) or PA-Av-C3d₃/IFA (black columns). Error bars represent SEM (n = 3) (*p≤0.05, **p≤0.01, ***p≤0.005).</p

PA-Av-C3d₃ provides enhanced protection against aerosol anthrax spore challenge.

<p>A/J mice were immunized with either: adjuvant control (*), PA-Av/IFA (○), PA-Av-Alum (□), or PA-Av-C3d₃/IFA (▵). Ten days after immunization, mice were challenged with (A) 5×10⁵ (10–40x LD₅₀, n = 5 per group) or (B) 5×10⁶ (100–400×LD_50, n = 10 per group) Sterne <i>B. anthracis</i> spores administered intranasally. Mouse survival was monitored for 10 days post-spore challenge.</p

Immunization with PA-Av-C3d₃/IFA augments production of PA- and Domain 4-specific antibodies relative to alum.

<p>Groups of A/J (top), BALB/c (middle), and C3H/HeJ (bottom) mice were immunized with PA-Av/IFA (○), PA-Av-Alum (□), or PA-Av-C3d₃/IFA (▵). (A) Serum PA- and (B) Domain 4-specific IgG was measured by ELISA (Abs 405 nm). All mice were boosted at day 42 (↑) with PA in saline. Error bars indicate SEM (n = 5). (C) Endpoint ELISAs were performed on day 14 serum samples from PA-Av/IFA, PA-Av-Alum, and PA-Av-C3d₃/IFA immunized mice. Endpoint titers shown are 2-fold above background. Error bars represent SEM (n = 5, *p≤0.05, ** p≤0.01, *** p≤0.005).</p

Immunization with PA-Av-C3d₃/IFA augments production of isotype switched antibody.

<p>Domain 4-specific IgM, IgG1, IgG2a, IgG2b and IgG3 ELISAs (Abs 405 nm) were performed from A/J mice immunized with PA-Av/IFA (○), PA-Av-Alum (□), or PA-Av-C3d₃/IFA (▵). All error bars indicate SEM (n = 5).</p

C3d is a functional adjuvant in adolescent mice.

<p>3-week old A/J mice were immunized with PA-Av/IFA (○), PA-Av-Alum (□), or PA-Av-C3d₃/IFA (▵). (A) PA-specific IgG or (B) domain-4 specific IgG was measured by ELISA up to 20 days after primary immunization. Error bars indicate average of 2 mice/group, and was repeated twice.</p

PA-Av-C3d₃ is an effective immunogen delivered i.p., s.c or i.m.

<p>PA-Av-C3d₃ was injected i.p. (▵), s.c. (▪), or i.m. (♦) and (A) PA- or (B) Domain 4-specific IgG was measured by ELISA. Relative efficacy of PA-Av-C3d₃ (▪,♦) and PA-Av-alum (□,⋄) was compared following (C) s.c. or (D) i.m. administration of immunogen (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001044#s2" target="_blank">methods</a>). Error bars indicate SEM (*p≤0.05, ** p≤0.01, *** p≤0.005, n = 3).</p