《資料》大学生の古典力調査報告Ⅹ ―平成 29 年度横浜国立大学教育学部学校教育課程一年次生の古典に関する関心度調査―

departmental bulletin pape

英国における「ドイツのトゥルネン」 : ある文化伝播の挫折

departmental bulletin pape

Observation of the Color-Suppressed Decay B̅ 0→D0π0

journal articl

Transmission electron microscopic observation of nanoindentations made on ductile-machined silicon wafers

Nanoindentation tests were performed on a ductile-machined silicon wafer with a Berkovich　diamond indenter, and the resulting indents were examined with a transmission electron microscope. It was found that the machining-induced subsurface amorphous layer undergoes significant plastic flow， and the microstructure of the indent depends on the indentation load. At a small load (～20 mN)，most of the indented region remains to be amorphous with minor crystalline nuclei; while under a large load (～50 mN)，the amorphous phase undergoes intensive recrystallization. The understanding and utilization of this phenomenon might be useful for improving the microscopic surface properties of silicon parts produced by a ductile machining process.journal articl

Slumping Behavior of Fresh Concrete

application/pdfThe purpose of the present paper is to investigate a simulation method of the behavior of fresh concrete at its flowing. To estimate the flow and deformation of fresh concrete, a method of visco-plastic finite element analysis is proposed. The analysis is based on rheology in which fresh concrete is treated as a kind of highly cocentrated suspension and its properties can be expressed by the rheological constants of Bingham model. In this paper, some analytical results for the slump test, sphere-lifting viscometer, and parallel-plates plastometer are shown and discussed. The relationship between slump value and rheological constants is theoretically clarified, which has been reported only from experiments or very simple calculations. Further the consistency curves ontained by the analysis of some kinds of viscometers are discussed, and a few problems are pointed out for the measurements of rheological constants used by these methods. The experiments to measure the frictional force between fresh concrete and steel board, and to measure slump value-slumping time curves are also carried out. The relationships between rheological constants and mix proportion of concrete are shown by comparison of experimental and calculated results.departmental bulletin pape

ZHX3 is involved in senescent phenotypes.

(A) Schematic diagram of the Zhx3 gene locus in wild type (WT) and Zhx3-knockout (KO) alleles. Arrows indicate primers used for genotyping PCR. (B) Genotyping PCR of WT, heterozygous and homozygous Zhx3-KO mice. (C) RT-qPCR analysis of p16INK4a in tissues of 7-week-old WT and KO mice. Values shown are mean +/- standard deviation (each n = 3), using the Student’s t-test (*p(D) Kaplan-Meier survival curves of male WT and Zhx3-KO mice (each n = 9). (E) Proposed model of the role of ZHX3 in preventing cellular senescence. ZHX3 and its cofactors (ACLY and RNA metabolism proteins) suppress p16INK4a and ribosomal RNA gene transcription, which promotes mitochondrial–nucleolar activation in senescent cells.</p

ZHX3 cooperates with RNA metabolism proteins.

(A) Silver staining of proteins immunoprecipitated from nuclear extracts of proliferating IMR-90 cells using IgG and anti-ZHX3 antibodies. Mass spectrometry-based proteomics analyses identified 28 proteins that interacted with ZHX3; estimated bands for ZHX3 and ATP citrate lyase (ACLY) are indicated. (B) Gene Ontology analysis of the ZHX3-interacting proteins, which mostly comprised RNA-related proteins. (C) Western blot analysis of proteins immunoprecipitated using IgG and anti-ZHX3 antibodies from IMR-90 nuclear extracts, with (+) or without (-) RNase treatment. Antibodies against ZHX3, ACLY, PABPC1 and EIF4A3 were used for detection. (D) Representative immunofluorescent images of enlarged nucleoli in IMR-90 cells (day 6) with ZHX3-, ACLY-, EIF4A3-, or PABPC1-KD. DNA was counterstained with DAPI. Scale bar, 10 μm. Quantitative data of the cells that had enlarged nucleoli are shown in S3C Fig. (E) RT-qPCR analysis of p16INK4a and ribosomal RNA genes on day 6 of each KD in IMR-90 cells. Values shown are mean +/- standard deviation from three independent experiments, using the Student’s t-test (*p<0.05, **p<0.01).</p

Analysis of ZHX3 function.

(A) ChIP-qPCR analysis of ZHX3 at ribosomal DNA loci, and RT-qPCR analyses of ribosomal RNA in growing, RS and OIS cells. For RS, IMR-90 cells were repeatedly passaged for 10 weeks. OIS was induced by expressing oncogenic Ras (H-rasV12) for 6 days. (B) RT-qPCR analysis of individual KD of ZHX3, ACLY, EIF4A3 and PABPC1 compared with Control-KD in IMR-90 cells (day 3). Values shown are the mean +/- standard deviation from three independent experiments, using the Student’s t-test (*p(C) Quantitative data of the cells that had enlarged nucleoli are shown (each >20 cells). (TIF)</p

Original uncropped and unadjusted images.

(TIF)</p

Identification of proteins that interact with ZHX3 using mass spectrometry-based proteomics analysis.

Cellular proteins were immunoprecipitated with anti-ZHX3 antibodies, followed by liquid chromatography in tandem with mass spectrometry analyses. The number of Mascot scores, peptide hits and peptide-spectrum matches (PSMs) are included in the list. Non-specific proteins are shaded in gray. (TIF)</p