74 research outputs found
Solid-state NMR characterization of triacylglycerol and polysaccharides in coffee beans
It is important to understand the structural characteristics of triacylglycerol (TAG), polysaccharides and trace elements in coffee beans, so that residues can be reutilized in applications including biodiesel oils. Here, we performed 1H and 13C solid-state NMR measurements on Indonesian green beans, roasted beans, and spent coffee grounds (SCGs). In the NMR spectra, there were liquid-like TAG containing linoleic acids based on observed signals of -CH=CH-CH2-CH=CH- group in an acyl chain, which play a role in decreasing TAG’s melting point. We found TAG was still abundant in the SCGs from NMR spectra. After lipids were removed from SCGs, the intensity of the TAG signal decreased considerably, with approximately 64% of the TAG was successfully extracted. We described the chemical structure of TAG in coffee beans and demonstrated that it is possible quantify the amount of extracted TAG using solid-state NMR.
Triacylglycerol as a source of biodiesel fuel in spent coffee grounds, green and roasted coffee beans was thoroughly characterized by 1H and 13C solid-state NMR spectroscopy.journal articl
Additional file 4: of A new genomic library of melon introgression lines in a cantaloupe genetic background for dissecting desirable agronomical traits
Mean, standard deviation (SD) and range values of the number of male and female flowers 30 days after the opening of the first flower (NMaF30 and NFe30), days to maturity (DMat), fruit weight (FW), fruit length (FL), fruit diameter (FD), fruit shape (FS), cavity width (CW), flesh firmness (FF), presence of abscission layer (AL), aroma (AR), rind thickness (RTh), netting (Net), flesh color parameters (FCHL, FCa and FCb), color of the inner rind (CIR), soluble solids content (SSC), and sucrose, glucose and fructose content (SUC, GLUC and FRUC) of both parents, VED and MAK, their F1 and the IL population assayed in three experiments. In the VED and MAK data, asterisks in rows indicate significant mean differences between trials (p < 0.05), and in the columns between parents; ns (not significant differences), na (not available). Estimation of heritability (h2 = VarG/(VarG + VarE) was performed for each trait and environment by calculating the variance components from the mean squares (MS) within and between ILs with an ANOVA (MSbetween = VarE + n VarG and MSwithin = VarE, where VarG = genotypic variance, VarE = environmental variance, and n = number of plants per IL). Data of the ILs in the three environments were analyzed using a two-factor ANOVA that was performed to examine the effect of genotype, environment and genotype-x-environment interaction. The percentage of variance explained by each effect (genotype, environment and the interaction) is indicated (*p < 0.05, **p < 0.001 and ns (no significant differences)). (DOCX 43 kb
Additional file 1: of A new genomic library of melon introgression lines in a cantaloupe genetic background for dissecting desirable agronomical traits
Fruits of the two parents used to generate the IL population and the corresponding F1. From left to right: the cultivar Vedrantais (VED) (C. melo subsp. melo var. cantalupensis, Charentais type) used as recurrent parent, Ginsen makuwa (MAK) (C. melo subsp. agrestis var. makuwa) used as donor parent, and their F1. (PPTX 876 kb
Additional file 5: of A new genomic library of melon introgression lines in a cantaloupe genetic background for dissecting desirable agronomical traits
List of all QTLs detected with the Dunnet’s test in at least two of the three trials performed with the selected IL collection. Abbreviated trait name as in Additional file 4, QTL name, chromosome, QTL position (cM) and flanking markers, according to the phenotype of overlapping lines, number of trials in which the QTL was detected, MAK effect relative to the VED parental (%) with positive/negative effects indicating that MAK alleles increase/decrease the value of the trait, IL introgression position (cM) and flanking markers. (XLSX 15 kb
Additional file 4: of Re-evaluation of the role of Indian germplasm as center of melon diversification based on genotyping-by-sequencing analysis
Figure S2. STRUCTURE results for K = 2 to 5. The five groups defined by Multi Dimensional Scaling (MDS) analysis are indicated at the right with their respective colors. K = 5 showed the Delta K peak, defining five populations. Wild accessions are highlighted with a star with the color of their STRUCTURE populations. (PPTX 480 kb
Additional file 9: of Re-evaluation of the role of Indian germplasm as center of melon diversification based on genotyping-by-sequencing analysis
Table S4. Haplotypes blocks defined by SNPanalyzer 2.0 (XLSX 57 kb
Additional file 10: of Re-evaluation of the role of Indian germplasm as center of melon diversification based on genotyping-by-sequencing analysis
Figure S6. Genomic location of LD blocks on the melon genome. Blocks fixed in C. melo ssp. melo (M) and cantaloupe group (C) are indicated. Centromeric regions in the chromosomes are marked in black. (PPTX 164 kb
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