新規球状オリゴ糖鎖デンドリマーの合成と生理活性

北見工業大学博士（工学）doctoral thesi

A STUDY OF THE BONE MORPHOGENETIC PROTEIN DERIVED FROM BOVINE DEMINERALIZED DENTIN MATRIX

1996-03-29We tried to extract and purify bone morphogenetic protein (BMP) from bovine demineralized dentin matrix (DDM). Crude dentin BMP (d-BMP) was extracted from DDM in 3 sequential steps. Purification of crude d-BMP was carried out by liquid chromatography. The molecular weight and the isoelectric point of the purified d-BMP were determined by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and electrofocusing. All fractioned samples were bioassayed in the thigh muscle pouches of AKR strain mice to test their ability to induce new bone formation. A sequence of 3 extraction steps worked effectively to obtain crude d-BMP. The purified d-BMP was shown to be homogeneous on high performance liquid chromatography (HPLC) and SDS-PAGE. The molecular weight and the pI were 25 kDa and 6.5, respectively. The amino acid composition was different from that of known bone-derived BMP. The purified d-BMP induced new bone formation in the thigh muscle pouches. The molecular weight, pI and amino acid composition were different from those of bone-derived BMP.departmental bulletin pape

フランスにおける弁護士および司法官の職業倫理についての総論的報告 (立命館大学ヨーロッパ法セミナー『フランスにおける法曹倫理』)

departmental bulletin pape

<地域研究>ベルーナドームでのプロ野球観戦者が所沢市にもたらす経済効果

departmental bulletin pape

Ⅲ族窒化物半導体のバンドギャップに格子歪みが与える影響

application/pdf三重大学大学院 工学研究科 博士前期課程 機械工学専攻 先進物質・先進材料領域 量子・電子機械講座 量子物性工学研究室98pthesi

ハイヒール クツ チャクヨウジ ノ シンタイ ジュウシン ドウヨウ

application/pdfdepartmental bulletin pape

The effectiveness of pulsed electrical stimulation (E-PES) in the management of osteoarthritis of the knee: a protocol for a randomised controlled trial-2

-36 – Medical Outcomes Study 36-item Short-Form Survey; HAP – Human Activity Profile; GPES – Global Perceived Effectiveness Scale.<p><b>Copyright information:</b></p><p>Taken from "The effectiveness of pulsed electrical stimulation (E-PES) in the management of osteoarthritis of the knee: a protocol for a randomised controlled trial"</p><p>http://www.biomedcentral.com/1471-2474/9/18</p><p>BMC Musculoskeletal Disorders 2008;9():18-18.</p><p>Published online 4 Feb 2008</p><p>PMCID:PMC2275728.</p><p></p

The effectiveness of pulsed electrical stimulation (E-PES) in the management of osteoarthritis of the knee: a protocol for a randomised controlled trial-0

-36 – Medical Outcomes Study 36-item Short-Form Survey; HAP – Human Activity Profile; GPES – Global Perceived Effectiveness Scale.<p><b>Copyright information:</b></p><p>Taken from "The effectiveness of pulsed electrical stimulation (E-PES) in the management of osteoarthritis of the knee: a protocol for a randomised controlled trial"</p><p>http://www.biomedcentral.com/1471-2474/9/18</p><p>BMC Musculoskeletal Disorders 2008;9():18-18.</p><p>Published online 4 Feb 2008</p><p>PMCID:PMC2275728.</p><p></p