7 research outputs found
Incorporating abstract data types and complex objects in relational database environments
筑波大学University of Tsukuba博士(工学)Doctor of Philosophy in Engineering1990【要旨】thesi
テレノベラ El clon における二つの宗教 : イスラム教・キリスト教 : 人間の誕生に関する生命倫理を巡って
2012-10-11departmental bulletin pape
Table_1_Identification of QTLs associated with seed protein concentration in two diverse recombinant inbred line populations of pea.xlsx
Improving the seed protein concentration (SPC) of pea (Pisum sativum L.) has turned into an important breeding objective because of the consumer demand for plant-based protein and demand from protein fractionation industries. To support the marker-assisted selection (MAS) of SPC towards accelerated breeding of improved cultivars, we have explored two diverse recombinant inbred line (RIL) populations to identify the quantitative trait loci (QTLs) associated with SPC. The two RIL populations, MP 1918 × P0540-91 (PR-30) and Ballet × Cameor (PR-31), were derived from crosses between moderate SPC × high SPC accessions. A total of 166 and 159 RILs of PR-30 and PR-31, respectively, were genotyped using an Axiom® 90K SNP array and 13.2K SNP arrays, respectively. The RILs were phenotyped in replicated trials in two and three locations of Saskatchewan, Canada in 2020 and 2021, respectively, for agronomic assessment and SPC. Using composite interval mapping, we identified three QTLs associated with SPC in PR-30 and five QTLs in PR-31, with the LOD value ranging from 3.0 to 11.0. A majority of these QTLs were unique to these populations compared to the previously known QTLs for SPC. The QTL SPC-Ps-5.1 overlapped with the earlier reported SPC associated QTL PC-QTL-3. Three QTLs, SPC-Ps-4.2, SPC-Ps-5.1, and SPC-Ps-7.2 with LOD scores of 7.2, 7.9, and 11.3, and which explained 14.5%, 11.6%, and 11.3% of the phenotypic variance, respectively, can be used for marker-assisted breeding to increase SPC in peas. Eight QTLs associated with the grain yield were identified with LOD scores ranging from 3.1 to 8.2. Two sets of QTLs, SPC-Ps-2.1 and GY-Ps-2.1, and SPC-Ps-5.1 and GY-Ps-5.3, shared the QTL/peak regions. Each set of QTLs contributed to either SPC or grain yield depending on which parent the QTL region is derived from, thus confirming that breeding for SPC should take into consideration the effects on grain yield.</p
Distribution of phenotypic characteristics of the mutant population and rate of pleiotropy
Number of M2 families in each phenotypic group. The x-axis indicates the nine major phenotypic categories, listed in Table 2, and the y-axis indicates the total number of M2 families. Each bar represents the number of mutants in the corresponding category. The blue bar represents the quantity of pleiotropic mutants (having more than one phenotype), given by the first number in the category label. The red bar represents the non-pleiotropic mutants and is given by the second number in the category label. Total number of M2 families (y-axis) sharing 1-5 major phenotypic categories (x-axis). The bar for one phenotypic category indicates how many mutants are categorized in only one phenotypic group (non-pleiotropic mutants), and the bars for the 2-5 phenotypic categories represent the number of mutants that share two to five phenotypes, respectively. In each case, the total number of mutants is indicated on the top of the bar.<p><b>Copyright information:</b></p><p>Taken from "UTILLdb, a forward and reverse genetics tool"</p><p>http://genomebiology.com/2008/9/2/R43</p><p>Genome Biology 2008;9(2):R43-R43.</p><p>Published online 26 Feb 2008</p><p>PMCID:PMC2374714.</p><p></p
Examples of mutant phenotypes representing the nine major phenotypic groups
Plant 566: cotyledon color, albino. Plant 939: plantlet architecture, bushy; plant architecture, hyper compact; leaf color, pale green; stem size, extreme dwarf. Plant 54: plant architecture, determinate growth. Plant 1,236: plant architecture, basal branching; leaf color, pale green, yellow; leaf size, medium; stem size, dwarf. Plant 903: leaf, cone shaped at leaf base; flowers, sterile flowers. Plant 1,567: leaf, distorted; stipule, silver-argentous. Plant 630: flowers, cauliflower type inflorescence; flowers, abnormal all; stem, dwarf; leaf, upcurling.<p><b>Copyright information:</b></p><p>Taken from "UTILLdb, a forward and reverse genetics tool"</p><p>http://genomebiology.com/2008/9/2/R43</p><p>Genome Biology 2008;9(2):R43-R43.</p><p>Published online 26 Feb 2008</p><p>PMCID:PMC2374714.</p><p></p
