35 research outputs found
On the magnification and the Errors of the Electron Microscope
Get PDFWhen we measure the size of a fine particle as in the case of determining the particle size distribution with the electron microscope whose magnification is given from the objective and projector lens current, it is generally said that the errors can not be expected to be les than 10~20%. Those errors are originated from various reasons such as the image distortion, magnetic hysteresis effects in the lens pole pieces, daily changing various electrical conditions, soiling of the aperture diaphragm in the objective lens and some others. In order to minimanize these errors, however, we should be careful so as to choose a series of a good condition in each factor, and only by special precaution we can reduce the error to be less than 10%. The author scrutinized each of the causes of errors of an electron microscope of (S.M. – U.5)type, and determined the magnification from the objective and projector lens current, and some other conditions that would give the least possible errors. From the obtained results, the author found that the error could be reduced under about 3%. Yet, the magnification must be checked at least once a month in order to maintain its accuracy because of some the changeable factors mentioned above.departmental bulletin pape
Close correlation of 1-beta-D-arabinofuranosylcytosine 5'-triphosphate, an intracellular active metabolite, to the therapeutic efficacy of N(4)-behenoyl-1-beta-D-arabinofuranosylcytosine therapy for acute myelogenous leukemia.
Get PDFN(4)-Behenoyl-1-beta-D-arabinofuranosylcytosine (BHAC), a prodrug of 1-beta-D-arabinofuranosylcytosine, is used effectively for the treatment of leukemia in Japan. BHAC therapy may be more effective if it is delivered in conjunction with monitoring of 1-beta-D-arabinofuranosylcytosine 5'-triphosphate (ara-CTP), the intracellular active metabolite of ara-C derived from BHAC. However, previous monitoring methods for ara-CTP were insufficiently sensitive. Here, using our new sensitive method, we evaluated the ara-CTP pharmacokinetics in relation to the therapeutic response in 11 acute myelogenous leukemia patients who received a 2-h infusion of BHAC (70 mg / m(2)) in combination remission induction therapy. ara-CTP could be monitored at levels under 1 mM. BHAC maintained effective levels of plasma ara-C and intracellular ara-CTP for a longer time, even compared with historical values of high-dose ara-C. The area under the concentration-time curve of ara-CTP was significantly greater in the patients with complete remission than in the patients without response. This greater amount of ara-CTP was attributed to the higher ara-CTP concentrations achieved in the responding patients. There was no apparent difference of plasma ara-C pharmacokinetics between the two groups. Thus, for the first time, the ara-CTP pharmacokinetics was evaluated in relation to the therapeutic effect of BHAC, and the importance of ara-CTP was proven. Administration of optimal BHAC therapy may require monitoring of the ara-CTP pharmacokinetics in each individual patient.othe
(A) Cdc14B-GFP fusion proteins were induced by DOX for 72 h in U2OS Tet-On stable cell lines carrying different Cdc14B-GFP constructs as indicated
No full textCentrosomes were visualized by γ-tubulin staining (red) and overlaid with Cdc14B-GFP (green) and DAPI-stained DNA (blue). Cdc14B-GFP was not detectable at interphase or mitotic centrosomes (arrows). Insets represent magnified images of centrosomes. Bar, 5 μm. (B) Histogram shows the percentage of interphase and mitotic cells with the indicated Cdc14B-GFP at centrosomes 72 h after DOX addition. The interphase data represent the means ± SD of three independent experiments and at least 500 cells were counted in each experiment. The mitotic experiment was performed in triplicates and a total of 113 Cdc14B-GFP– and 374 Cdc14B-GFP–positive cells were counted.<p><b>Copyright information:</b></p><p>Taken from "Cdc14B depletion leads to centriole amplification, and its overexpression prevents unscheduled centriole duplication"</p><p></p><p>The Journal of Cell Biology 2008;181(3):475-483.</p><p>Published online 5 May 2008</p><p>PMCID:PMC2364701.</p><p></p
