The COMET Open-label Phase II Study of Neoadjuvant FOLFOX or XELOX Treatment Combined with Molecular Targeting Monoclonal Antibodies in Patients with Resectable Liver Metastasis of Colorectal Cancer

Background: Advantages of neoadjuvant chemotherapy combined with monoclonal antibodies for treating patients with resectable colorectal cancer liver metastasis (CLM) have not been established. The purpose of this study was to evaluate the efficacy and safety of oxaliplatin-based regimen (FOLFOX or XELOX) plus monoclonal antibodies (cetuximab or bevacizumab) treatment in patients with resectable CLM. Methods: A single-arm, open-label, multicenter, phase II trial was conducted for patients aged ≥ 20 years with resectable and untreated CLM. Patients received preoperative FOLFOX (6 cycles) or XELOX (4 cycles). Cetuximab or bevacizumab was administered to patients with wild-type or mutated KRAS codons 12 and 13, respectively. The primary endpoint was progression-free survival (PFS). Results: Between January 2010 and June 2012, 47 patients were enrolled from 12 institutions. Wild-type or mutant KRAS sequences were examined in 32 and 15 patients, respectively. Twenty-one (45 %) patients experienced Grades 3/4 adverse events, and 55 % of all patients responded to therapy. The sizes of tumors of patients in the wild-type KRAS group were significantly reduced compared with those of the mutant KRAS group. The overall rates of liver resection and postoperative morbidity were 83 and 14 %, respectively, and the median PFS was 15.6 months. The median PFS times of the KRAS wild-type and mutant groups were 22.5 months and 10.5 months, respectively. Conclusions: Neoadjuvant therapy using FOLFOX/XELOX combined with monoclonal antibodies did not improve PFS, although it was administered safely and had less adverse effects after liver resection.journal articl

Generation and Measurement of Picosecond Laser Pulses (III) -Oscillation and Amplification of a CPM G1ass Laser -

Ultra-short light pulses of several picoseconds are easily obtained from a conventional selιmode-locked Nd3+ glass laser. However, we tried to improve the pulse-width further by use of CPM (Colliding Pulse Mode-locking) method, as a new proposal, in which a saturable absorber cell of 1 mm thick was set in the centre of the laser cavity of 1 m length and a glass rod (LHG-8, 5φ,75ι) was between the cell and the coupling mirror. We used the dye NDL-112 as a saturable absorber of self-mode-locking. The pulse-width was measured by observation of TPF patterns in Rh-6G cell. By exact adjustment of the cell position, we was able to get a long train of about 100 pulses per shot,of which the pulse-width was less than 3 psec, just a half of the conventional.departmental bulletin pape

岩手大学「持続可能な社会のための教養教育の再構築」『学びの銀河』プロジェクト最終報告書

research repor

A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors-3

<p><b>Copyright information:</b></p><p>Taken from "A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors"</p><p>http://www.plantmethods.com/content/3/1/7</p><p>Plant Methods 2007;3():7-7.</p><p>Published online 8 Jun 2007</p><p>PMCID:PMC1914063.</p><p></p>itions' includes all probes tested (different cultivars, tissues and physiological conditions); 'Root tissue (100 mM salt stress)' includes all root samples of salt stress-treated plants; 'Root tissue' and 'Shoot tissue' include samples of non-stressed tissues of different cultivars. A lower M value indicates more stable expression. According to the geNORM manual, genes with M values >1.5 are not suitable reference genes for the selected conditions. Gene codes and primers used for the qRT-PCR experiments are given in Table 1

A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors-2

<p><b>Copyright information:</b></p><p>Taken from "A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors"</p><p>http://www.plantmethods.com/content/3/1/7</p><p>Plant Methods 2007;3():7-7.</p><p>Published online 8 Jun 2007</p><p>PMCID:PMC1914063.</p><p></p> ng) and used as template to test transcript abundance of three selected genes (Os03g55610, Os08g38220, and Os12g38200) via qRT-PCR. A linear relationship between root (or shoot) cDNA and expression level of the various genes was observed. Symbols in both panels represent the mean ± SD (= 3)

A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors-1

<p><b>Copyright information:</b></p><p>Taken from "A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors"</p><p>http://www.plantmethods.com/content/3/1/7</p><p>Plant Methods 2007;3():7-7.</p><p>Published online 8 Jun 2007</p><p>PMCID:PMC1914063.</p><p></p>erent at p = 0.001. Non-parametric comparison of mean values (Mann-Whitney U test) confirmed the presence of statistically significant differences at p = 0.000001. Transformation to expression values revealed that the slightly different PCR efficiencies could lead to a mean difference of maximal 0.3, when the fold change was expressed as log. Individual primer pairs can thus exhibit slight differences for their target genes in different cultivars. However, this does not significantly affect the overall applicability of the primer platform for expression profiling experiments (Caldana ., manuscript in preparation)

A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors-0

<p><b>Copyright information:</b></p><p>Taken from "A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors"</p><p>http://www.plantmethods.com/content/3/1/7</p><p>Plant Methods 2007;3():7-7.</p><p>Published online 8 Jun 2007</p><p>PMCID:PMC1914063.</p><p></p>DNA (gDNA), evaluation of cDNA quality, primer design and data analysis. The absence of gDNA was confirmed by quantitative RT-PCR (qRT-PCR) with primer pairs targeting various non-coding regions. The quality of the cDNA was tested using different reference genes, as outlined in the text

5 当院における小腸内視鏡検査の現状(II.主題,第64回新潟大腸肛門病研究会)

departmental bulletin pape

Table_4_Application of Synthetic Peptide CEP1 Increases Nutrient Uptake Rates Along Plant Roots.xlsx

The root system of a plant provides vital functions including resource uptake, storage, and anchorage in soil. The uptake of macro-nutrients like nitrogen (N), phosphorus (P), potassium (K), and sulphur (S) from the soil is critical for plant growth and development. Small signaling peptide (SSP) hormones are best known as potent regulators of plant growth and development with a few also known to have specialized roles in macronutrient utilization. Here we describe a high throughput phenotyping platform for testing SSP effects on root uptake of multiple nutrients. The SSP, CEP1 (C-TERMINALLY ENCODED PEPTIDE) enhanced nitrate uptake rate per unit root length in Medicago truncatula plants deprived of N in the high-affinity transport range. Single structural variants of M. truncatula and Arabidopsis thaliana specific CEP1 peptides, MtCEP1D1:hyp4,11 and AtCEP1:hyp4,11, enhanced uptake not only of nitrate, but also phosphate and sulfate in both model plant species. Transcriptome analysis of Medicago roots treated with different MtCEP1 encoded peptide domains revealed that hundreds of genes respond to these peptides, including several nitrate transporters and a sulfate transporter that may mediate the uptake of these macronutrients downstream of CEP1 signaling. Likewise, several putative signaling pathway genes including LEUCINE-RICH REPEAT RECPTOR-LIKE KINASES and Myb domain containing transcription factors, were induced in roots by CEP1 treatment. Thus, a scalable method has been developed for screening synthetic peptides of potential use in agriculture, with CEP1 shown to be one such peptide.</p

Table_3_Application of Synthetic Peptide CEP1 Increases Nutrient Uptake Rates Along Plant Roots.xlsx

The root system of a plant provides vital functions including resource uptake, storage, and anchorage in soil. The uptake of macro-nutrients like nitrogen (N), phosphorus (P), potassium (K), and sulphur (S) from the soil is critical for plant growth and development. Small signaling peptide (SSP) hormones are best known as potent regulators of plant growth and development with a few also known to have specialized roles in macronutrient utilization. Here we describe a high throughput phenotyping platform for testing SSP effects on root uptake of multiple nutrients. The SSP, CEP1 (C-TERMINALLY ENCODED PEPTIDE) enhanced nitrate uptake rate per unit root length in Medicago truncatula plants deprived of N in the high-affinity transport range. Single structural variants of M. truncatula and Arabidopsis thaliana specific CEP1 peptides, MtCEP1D1:hyp4,11 and AtCEP1:hyp4,11, enhanced uptake not only of nitrate, but also phosphate and sulfate in both model plant species. Transcriptome analysis of Medicago roots treated with different MtCEP1 encoded peptide domains revealed that hundreds of genes respond to these peptides, including several nitrate transporters and a sulfate transporter that may mediate the uptake of these macronutrients downstream of CEP1 signaling. Likewise, several putative signaling pathway genes including LEUCINE-RICH REPEAT RECPTOR-LIKE KINASES and Myb domain containing transcription factors, were induced in roots by CEP1 treatment. Thus, a scalable method has been developed for screening synthetic peptides of potential use in agriculture, with CEP1 shown to be one such peptide.</p