産学官連携主導型医工連携によるスタンドレス輸液装置開発と課題解決

岩手大学博士（工学）doctoral thesi

Jasmonic acid facilitates flower opening and floral organ development through the upregulated expression of SlMYB21 transcription factor in tomato

Plants coordinate the timing of flower opening with pollen and gynoecium maturation to achieve successful pollination. However, little is known about how the coordination is executed. We found that flower bud development was paused immediately before flower opening in a jasmonic acid (JA)-insensitive tomato mutant, jai1-1. Phytohormone measurement and RNA analysis in flower buds revealed that newly synthesised JA peaked at two days before flower opening and the expression of a transcription factor gene SlMYB21 delayed in jai1-1. Buds of transgenic tomato plants expressing an artificial repressor, AtMYB24-SRDX, which was expected to impede the function of SlMYB21, aborted flower opening and resembled those of jai1-1. Furthermore, the AtMYB24-SRDX plants produced abnormal pollen grains deficient in germination and pistils that did not support pollen tube elongation. We concluded that JA facilitates the expression of SlMYB21, which coordinates flower opening, pollen maturation, and gynoecium function in tomato. Jasmonic acid stimulates SlMYB21 expression, which is required for coordinated flower opening and fertility in male and female organs in tomato.ファイル公開：2019/02/15journal articl

ESR/NMR Development of double resonance system for observation of 31p nuclear spin state in low concentration Si:P and ESR measurement of dynamic nuclear polarizatio

We constructed an ESR/NMR double-resonance system for liquid-4He temperatures in order to detect a 31P-DNP-NMR signal from lightly doped (insulator) Si:P samples. We measured steady-state ESR of insulator samples of Si:P with the newly made system. DNP effect on ESR spectrum was found at 1.5 K. We also found that the magnitude of the nuclear spin polarization was changed by applying NMR pulses.research repor

北海道朱鞠内川流域に分布する白亜系の有孔虫化石

Articledepartmental bulletin pape

Jasmonic acid facilitates flower opening and floral organ development through the upregulated expression of SlMYB21 transcription factor in tomato

Plants coordinate the timing of flower opening with pollen and gynoecium maturation to achieve successful pollination. However, little is known about how the coordination is executed. We found that flower bud development was paused immediately before flower opening in a jasmonic acid (JA)-insensitive tomato mutant, jai1-1. Phytohormone measurement and RNA analysis in flower buds revealed that newly synthesised JA peaked at two days before flower opening and the expression of a transcription factor gene SlMYB21 delayed in jai1-1. Buds of transgenic tomato plants expressing an artificial repressor, AtMYB24-SRDX, which was expected to impede the function of SlMYB21, aborted flower opening and resembled those of jai1-1. Furthermore, the AtMYB24-SRDX plants produced abnormal pollen grains deficient in germination and pistils that did not support pollen tube elongation. We concluded that JA facilitates the expression of SlMYB21, which coordinates flower opening, pollen maturation, and gynoecium function in tomato. Jasmonic acid stimulates SlMYB21 expression, which is required for coordinated flower opening and fertility in male and female organs in tomato.ファイル公開：2019/02/15journal articl

Relationship of KLHDC4 expression with clinicopathological characteristics in NPC.

Relationship of KLHDC4 expression with clinicopathological characteristics in NPC.</p

KLHDC4 does not interact with Cullin2 or Cullin3.

(A-B) 293T cells were transiently tranfected with plasmids encoding SFB-tagged KLHDC3, KLHDC4, KLHDC10, KEAP1, or empty vector together with plasmid encoding HA-tagged Cullin2 or Cullin3 as indicated. Cell lysates were precipitated with S-protein beads and immunoblotted with indicated antibodies. (C) Protein structures of KLHDC4, KLHDC3, KLHDC10 and KEAP1. Red rectangle: Kelch repeat; Blue hexagon: BC box; Yellow rhombus: BTB domain; Green rhombus: BACK domain. (TIF)</p

Loss of KLHDC4 induces apoptosis in NPC cells.

<p>(A) Control and KLHDC4 KO cells were stained with DAPI and photographed by fluorescence microscopy under fluorescence and light fields. Left panel: representative images; the arrows indicated the condensed or fragmented nuclear and multiblebbing cells. Right panels: quantification of cells with condensed nuclear per field. **<i>P</i> <0.01. (B) Representative flow cytometric analysis of apoptotic CNE2 control and KLHDC4 KO cells stained for annexin V-FITC/PI under both non-treated and cis-platin treated conditions. The results are summarized in the right panel. Bars denote the S.E.M. (C) Western blot analysis of the expression of cleaved caspase-3, cleaved PARP and GAPDH, as the loading control, under both non-treated and cis-platin treated conditions. (D) Immunohistochemical analysis of KLHDC4, cleaved caspase-3, cleaved PARP, and Ki-67 expression in xenografts generated from CNE2 control and KLHDC4 KO cells. Left panels: representative images; Scale bars: 100 μm. Right panels: quantification of average percentage of cells with positive staining per field; CC3: cleaved caspase-3; CP: cleaved PARP. ***<i>P</i> <0.001.</p

Loss of KLHDC4 reduces NPC cell migration and invasion.

<p>(A) Wound-healing assays were performed at 0 and 20 hours with control and KLHDC4 KO cells. Left panels: representative images; Scale bars: 50 μm. Right panels: quantification of the wound closure area calculated by measuring the decreaseinthe wound bed surface overtime. **<i>P</i> <0.01. (B) KLHDC4 KO significantly reduced the invasive ability of CNE2 cells. Left panels: representative images; Scale bars: 50 μm. Right panels: quantification of average number of cells per field. ***<i>P</i> <0.001.</p

KLHDC4 expression correlation in NPC and adjacent nasopharyngeal epithelia.

<p>KLHDC4 expression correlation in NPC and adjacent nasopharyngeal epithelia.</p