185 research outputs found
Self-efficacy is an independent predictor for postoperative six-minute walk distance after elective open repair of abdominal aortic aneurysm
Objectives: Open surgery is performed to treat abdominal aortic aneurysm (AAA), although the subsequent surgical stress leads to worse physical status. Preoperative self-efficacy has been reported to predict postoperative physical status after orthopedic surgery; however, it has not been sufficiently investigated in patients undergoing abdominal surgery. The purpose of the present study is to investigate the correlation between preoperative self-efficacy and postoperative six-minute walk distance (6MWD) in open AAA surgery. Methods: Seventy patients who underwent open AAA surgery were included. Functional exercise capacity was measured using preoperative and 1 week postoperative 6MWD. Self-efficacy was preoperatively measured using self-efficacy for physical activity (SEPA). The correlations of postoperative 6MWD with age, height, BMI, preoperative 6MWD, SEPA, Hospital Anxiety and Depression Scale (HADS) score, operative time, and blood loss were investigated using multivariate analysis. Results: Single regression analysis showed that postoperative 6MWD was significantly correlated with age (r = −0.553, p ≤ 0.001), height (r = 0.292, p = 0.014), Charlson’s comorbidity index (r = −0.268, p = 0.025), preoperative 6MWD (r = 0.572, p ≤ 0.001), SEPA (r = 0.586, p ≤ 0.001), and HADS-depression (r = −0.296, p = 0.013). Multiple regression analysis showed that age (p = 0.002), preoperative 6MWD (p = 0.013), and SEPA (p = 0.043) score were significantly correlated with postoperative 6MWD. Conclusions: Self-efficacy was an independent predictor for postoperative 6MWD after elective open AAA surgery. This suggests the importance of assessing not only physical status but also psychological factors such as self-efficacy.Published online: 21 Feb 2017journal articl
ESR/NMR Development of double resonance system for observation of 31p nuclear spin state in low concentration Si:P and ESR measurement of dynamic nuclear polarizatio
We constructed an ESR/NMR double-resonance system for liquid-4He temperatures in order to detect a 31P-DNP-NMR signal from lightly doped (insulator) Si:P samples. We measured steady-state ESR of insulator samples of Si:P with the newly made system. DNP effect on ESR spectrum was found at 1.5 K. We also found that the magnitude of the nuclear spin polarization was changed by applying NMR pulses.research repor
Additional file 2: of Functional analysis of the promoter of an early zygotic gene KLC2 in Aedes aegypti
Luciferase expression in seven independent transgenic lines. Firefly luciferase assays showed that except JT0311-F2, other transgenic lines all have two expression peaks during the mosquito life-cycle. The first one is in early embryos and the second one is in pupa or adult males. (PNG 99 kb
Additional file 6: of Functional analysis of the promoter of an early zygotic gene KLC2 in Aedes aegypti
The sequence of the luciferase reporter cassette. (DOCX 116 kb
Additional file 5: of Functional analysis of the promoter of an early zygotic gene KLC2 in Aedes aegypti
Alignment of RNAseq data [29] to the genomic region surrounding the insertion site. The grey bars in the top panel indicate the alignment of iPCR sequence to the genome, which enabled the identification of the insertion site. The mapping results of various RNAseq data showed that the genes in the surrounding region were not transcribed in only males. Thus, the observed testis-biased expression is unlikely caused by enhancers or promoters in the neighboring region. (PNG 93 kb
Additional file 1: of Functional analysis of the promoter of an early zygotic gene KLC2 in Aedes aegypti
Marker phenotypes of the seven transgenic lines. (XLSX 27 kb
Additional file 4: of Functional analysis of the promoter of an early zygotic gene KLC2 in Aedes aegypti
Expression profile of KLC2 (AAEL011410) based on RNA sequencing data adopted from (a) Biedler et al. [25] and (b) Akbari et al. [29]. Both RNAseq data showed pure early zygotic expression of KLC2 without expression in adult males or male testes. (PNG 95 kb
Additional file 3: of Functional analysis of the promoter of an early zygotic gene KLC2 in Aedes aegypti
Primers and probes used in this study. (DOCX 19 kb
Cloning, characterization, and expression of microRNAs from the Asian malaria mosquito, -3
Signs indicate conservation. The mature miRNA is marked in red while miRNA* is marked in blue. Note the perfect conservation of the mature miRNA (red), high conservation of the miRNA* sequence (blue), and lower conservation of the surrounding stem and loop structure, a hallmark conservation pattern of pre-miRNAs. Shown on the right are the predicted secondary structures of corresponding miRNA hairpins. The mature miRNA is marked in red on the hairpin.<p><b>Copyright information:</b></p><p>Taken from "Cloning, characterization, and expression of microRNAs from the Asian malaria mosquito, "</p><p>http://www.biomedcentral.com/1471-2164/9/244</p><p>BMC Genomics 2008;9():244-244.</p><p>Published online 23 May 2008</p><p>PMCID:PMC2430712.</p><p></p
The non-LTR retrotransposon in mosquitoes: genomic impact, vertical transmission and indications of recent and widespread activity-1
<p><b>Copyright information:</b></p><p>Taken from "The non-LTR retrotransposon in mosquitoes: genomic impact, vertical transmission and indications of recent and widespread activity"</p><p>http://www.biomedcentral.com/1471-2148/7/112</p><p>BMC Evolutionary Biology 2007;7():112-112.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1947958.</p><p></p>Vg-C from is used to root the tree. Clade credibility values from 150,000 generations are given at each node. B. Consensus tree constructed with MrBayes using conceptually translated sequences of from PCR and genomic database (Aa, , Ag, ). Clade credibility values from 200,000 generations are given at each node or beside brackets. Ag-Jen-4 and other sequences correspond to families previously identified (Biedler and Tu 2003). Jockey elements from (Dm) and (Ct) are used to root the tree. Accessions are given beside sequence names. An asterisk indicates that the reading frame was intact. A bold capital "L" indicates that the sequence was obtained from a genomic library. The first three letters of a species name is used to label PCR and library sequences. Genus names are in bold beside brackets. is from and is from . Abbreviations: (); (); (); (); (); (); (); (); (); (); (); (); (); (); (); (); (). Number indicates clone from PCR. and sequences from genomic database (e.g. Ag-Jock-1, AaJockeyEle2) can be found in the TEfam database [57]. See additional files and for alignments used for phylogenetic inference
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