Trichothiodystrophy, complementation group A complicated with squamous cell carcinoma

ファイル公開：2019-02-01journal articl

Detection of Terahertz Pulsed Radiation by Using Heterodyne Electro-Optic Sampling Scheme

A new electro-optic(EO) sampling scheme, which we refer to as “'heterodyne EO sampling" for detection of pulsed terahertz (THz) waves, is proposed and demonstrated. In this heterodyne EO sampling scheme,the intensity change in the sampling optica1 pulse induced by a THz field in a nonlinear crystal is measured without using any polarization optics. Applied in conjunction with non-collinear Cherenkov-phase matching technique,this method allows for the detection of pulsed THz wave efficiently and easily using a simpler optical setup compared with the conventional EO sampling method.research repor

教職選択行動の規定因に関する2、3の検討

departmental bulletin pape

Trichothiodystrophy, complementation group A complicated with squamous cell carcinoma

ファイル公開：2019-02-01journal articl

Calpain 1 Binding Capacities of the N1-Line Region of Titin Are Significantly Enhanced by Physiological Concentrations of Calcium

Calpain 1, an ubiquitous well-known calcium-dependent intracellular protease, was recently shown to bind tightly to the proximal end of the I-band titin segment in a calcium-dependent manner [Raynaud et al. (2005) FEBS J. 272, 2578−2590]. In the present work we identified the titin Ig-domain of concern by this interaction and the role of calcium in this interaction using a recombinant fragment of titin spanning the I2−I6 region and its subfragments. The heterodimeric form of calpain 1 binds to this titin fragment with a very high affinity (Kd = 5.1 ± 0.2 × 10−7 M) at much lower calcium levels than those saturating the high-affinity binding sites of the peptidase (Kd = 25 μM). Investigation of this interaction with I2−I6 subfragments clearly showed that the dimeric form of calpain 1 binds exclusively to the Ig-domain I4 of titin with an affinity similar to that of the whole I2−I6 segment. As for the I2−I6 fragment, this interaction is calcium regulated. Calcium was shown to bind tightly to titin (Kd = 1.9 × 10−7 M), causing an oligomerization of the titin segment. At physiological calcium concentration (10−6 to 10−8 M), the prevailing form of the titin fragment is a trimer, suggesting that calpain 1 binds to this titin structure. From the present findings, it was concluded that calcium binding to titin increased the amount of bound calpain 1 (up to 40% of the total calpain 1) and that this bound calpain 1 might constitute a reservoir for this peptidase. In this context, we proposed a schematic diagram of this series of calcium-dependent events with the inherent unanswered questions. These events are probably under a complex regulation involving undoubtedly different yet unidentified proteins

An original gene cluster: Elucidation of genomic organization and gene expression in the 21q24 region-6

D I. Southern blot analysis of DNA from two overlapping BAC clones bI0123B08 and bI0511D06 digested with the same restriction endonucleases.<p><b>Copyright information:</b></p><p>Taken from "An original gene cluster: Elucidation of genomic organization and gene expression in the 21q24 region"</p><p>http://www.biomedcentral.com/1471-2164/9/151</p><p>BMC Genomics 2008;9():151-151.</p><p>Published online 2 Apr 2008</p><p>PMCID:PMC2373789.</p><p></p

An original gene cluster: Elucidation of genomic organization and gene expression in the 21q24 region-5

Oted on porcine SERPINA3s. SERPINA phylogeny based on ML method with the closest relatives of SERPINA3. The best ML tree (LnL = -16085.84) is unrooted. Bootstrap proportions are given for nodes supported in at least 50% cases. BP supporting clades are circled.<p><b>Copyright information:</b></p><p>Taken from "An original gene cluster: Elucidation of genomic organization and gene expression in the 21q24 region"</p><p>http://www.biomedcentral.com/1471-2164/9/151</p><p>BMC Genomics 2008;9():151-151.</p><p>Published online 2 Apr 2008</p><p>PMCID:PMC2373789.</p><p></p

An original gene cluster: Elucidation of genomic organization and gene expression in the 21q24 region-3

in the alignment, above the sequences, and to the length of each sequence, at the end of the line. Dot indicates a common residue with that of the first sequence (bovSERPINA3-1), dash shows a gap, i.e. a position with an insertion-deletion event. Shaded characters indicate selectively conserved residues specific of the sub-group including bovSERPINA3-7 and bovSERPINA3-8. (▼) indicates asparagine (N) residue involved in a potential N-glycosylation site.<p><b>Copyright information:</b></p><p>Taken from "An original gene cluster: Elucidation of genomic organization and gene expression in the 21q24 region"</p><p>http://www.biomedcentral.com/1471-2164/9/151</p><p>BMC Genomics 2008;9():151-151.</p><p>Published online 2 Apr 2008</p><p>PMCID:PMC2373789.</p><p></p

An original gene cluster: Elucidation of genomic organization and gene expression in the 21q24 region-2

characters and the names of loci established for Btau-2.0 genomic sequence assembly are added (when known) below between brackets. The different genes are localized on the four overlapping BAC clones covering approximately 235 Kb. The two question marks indicate the uncertainties of relative location for the genes /and /, respectively. The gene is also shown.<p><b>Copyright information:</b></p><p>Taken from "An original gene cluster: Elucidation of genomic organization and gene expression in the 21q24 region"</p><p>http://www.biomedcentral.com/1471-2164/9/151</p><p>BMC Genomics 2008;9():151-151.</p><p>Published online 2 Apr 2008</p><p>PMCID:PMC2373789.</p><p></p

An original gene cluster: Elucidation of genomic organization and gene expression in the 21q24 region-0

D I. Southern blot analysis of DNA from two overlapping BAC clones bI0123B08 and bI0511D06 digested with the same restriction endonucleases.<p><b>Copyright information:</b></p><p>Taken from "An original gene cluster: Elucidation of genomic organization and gene expression in the 21q24 region"</p><p>http://www.biomedcentral.com/1471-2164/9/151</p><p>BMC Genomics 2008;9():151-151.</p><p>Published online 2 Apr 2008</p><p>PMCID:PMC2373789.</p><p></p