Hematological Regulation Using β-aminobutyric Acid In Staphylococcus aureus infected Rats

Several studies have directed the use of different chemicals as an antibiotics alternative that kills bacteria or may lead to stimulating the body's resistance against infections, such as organic acids that include non-protein amino acids especially β-aminobutyric acid (ΒABA), which  has proven in this study its ability  to increase  the systemic resistance of male rats against  Staphylococcus aureus. As well as increasing the numbers of white blood cells and lymphocytes and their positive effect on improving the immune complement system . The aim of study was to know the effect of  β-aminobutyric Acid on the response immune system of animals that was infected  with   s. aureus bacteria Objectives of the study Study of hematological parameters of animals treated with BABA, including: WBC ,RBC , Hb , PCV ,LYM ,GRA     Materials used in  this experiment mannitol salt field, as well as blood agar and MacConkey agar were used for the development of Staphylococcus aureus bacteria, as well as amino acid β-aminobutyric Acid with different concentrations Prepare the first solution of amino acid concentration (20 mg/ml).The other amino acid was prepared at a concentration of (40 mg/ml)Then the required doses for each animal were prepared according to the weight of each animal Results: The effect of BABA on the level of  (RBC – WBC – PLT –LYM –GRA) The results showed that there was no effect of the amino acid BABA on the level of (RBC-MCV-PLT) in groups infected with bacteria and treated with BABAThe results showed that there was no effect of the amino acid BABA on the level of (WBC) in the groups infected with bacteria and treated with BABA, while the percentage of (LYM-GRA) increased

Comparison of diagnostic tests for the detection of Brucella spp. in camel sera

<p>Abstract</p> <p>Background</p> <p>Brucellosis in livestock causes enormous losses for economies of developing countries and poses a severe health risk to consumers of dairy products. Little information is known especially on camel brucellosis and its impact on human health. For surveillance and control of the disease, sensitive and reliable detection methods are needed. Although serological tests are the mainstay of diagnosis in camel brucellosis, these tests have been directly transposed from cattle without adequate validation. To date, little information on application of real-time PCR for detection of <it>Brucella </it>in camel serum is available. Therefore, this study was performed to compare the diagnostic efficiency of different serological tests and real-time PCR in order to identify the most sensitive, rapid and simple combination of tests for detecting <it>Brucella </it>infection in camels.</p> <p>Findings</p> <p>A total of 895 serum samples collected from apparently healthy Sudanese camels was investigated. Sudan is a well documented endemic region for brucellosis with cases in humans, ruminants, and camels. Rose Bengal Test (RBT), Complement Fixation Test (CFT), Slow Agglutination Test (SAT), Competitive Enzyme Linked Immunosorbant Assay (cELISA) and Fluorescence Polarization Assay (FPA) as well as real-time PCR were used. Our findings revealed that <it>bcsp31 </it>kDa real-time PCR detected <it>Brucella </it>DNA in 84.8% (759/895) of the examined samples, of which 15.5% (118/759) were serologically negative. Our results show no relevant difference in sensitivity between the different serological tests. FPA detected the highest number of positive cases (79.3%) followed by CFT (71.4%), RBT (70.7%), SAT (70.6%) and cELISA (68.8%). A combination of real-time PCR with one of the used serological tests identified brucellosis in more than 99% of the infected animals. 59.7% of the examined samples were positive in all serological tests and real-time PCR. A subpopulation of 6.8% of animals was positive in all serological tests but negative in real-time PCR assays. The high percentage of positive cases in this study does not necessarily reflect the seroprevalence of the disease in the country but might be caused by the fact that the camels were imported from brucellosis infected herds of Sudan, accidentally. Seroprevalence of brucellosis in camels should be examined in confirmatory studies to evaluate the importance of brucellosis in this animal species.</p> <p>Conclusion</p> <p>We suggest combining <it>bcsp31 </it>real-time PCR with either FPA, CFT, RBT or SAT to screen camels for brucellosis.</p

Hematological Regulation Using β-aminobutyric Acid In Staphylococcus aureus infected Rats

Several studies have directed the use of different chemicals as an antibiotics alternative that kills bacteria or may lead to stimulating the body's resistance against infections, such as organic acids that include non-protein amino acids especially β-aminobutyric acid (ΒABA), which  has proven in this study its ability  to increase  the systemic resistance of male rats against  Staphylococcus aureus. As well as increasing the numbers of white blood cells and lymphocytes and their positive effect on improving the immune complement system . The aim of study was to know the effect of  β-aminobutyric Acid on the response immune system of animals that was infected  with   s. aureus bacteria&#x0D; Objectives of the study&#x0D; Study of hematological parameters of animals treated with BABA, including:&#x0D; WBC ,RBC , Hb , PCV ,LYM ,GRA&#x0D;  &#x0D;  &#x0D; Materials used in  this experiment mannitol salt field, as well as blood agar and MacConkey agar were used for the development of Staphylococcus aureus bacteria, as well as amino acid β-aminobutyric Acid with different concentrations&#x0D; Prepare the first solution of amino acid concentration (20 mg/ml).The other amino acid was prepared at a concentration of (40 mg/ml)Then the required doses for each animal were prepared according to the weight of each animal&#x0D; Results:&#x0D; The effect of BABA on the level of  (RBC – WBC – PLT –LYM –GRA)&#x0D; The results showed that there was no effect of the amino acid BABA on the level of (RBC-MCV-PLT) in groups infected with bacteria and treated with BABAThe results showed that there was no effect of the amino acid BABA on the level of (WBC) in the groups infected with bacteria and treated with BABA, while the percentage of (LYM-GRA) increased.</jats:p

Improvement of Output Voltage From Shading Interference on Solar Cell Using A Reflector System

One of the factors that affect the performance of photovoltaic cells is shading. Shading can reduce the intensity of solar radiation on the cells. This study aims to design a system that can improve the amount of voltage from shading disturbances to optimise the output voltage on the solar cell by using a reflector. The reflector is designed in a flat mirror measuring. It is expected that there will be an increase in the output voltage of the existing solar cell system. The more reflected light that hits the surface of the solar panel and illuminates the shading area, the more significant the increase in output voltage, current and power, and vice versa