COVID-19 is Associated with Increased Severity in Pregnant Women

Background: COVID-19 pandemic originated in China in late 2019, the number of cases are increasing with 2,104,346 cases and 116,140 deaths in the United States, as of June 16, 2020. Pregnant women are a vulnerable population in epidemics or Pandemics. This Review is designed to look in detail the severity of COVID-19 in pregnant women in comparison to non-pregnant women of reproductive age. Methods: Literature search on PubMed, Google Scholar, Lancet, and Web of Science were conducted. Results: We have found the evidence of increased risk for severe disease and distinctive symptoms among pregnant women diagnosed with COVID-19 as compared to non-pregnant women. Conclusions: COVID-19 presents in an atypical fashion in pregnant women with comparatively increased severity of symptoms, compared to COVID-19 positive non pregnant women of reproductive age. These findings can help clinicians to recognize the risk posed by COVID-19 in pregnant women

COVID-19 & Pregnancy Complication During Early Pandemic: A Narrative Review

Background: Coronaviruses have caused 3 outbreaks in the past 2 decades. The novel one is SARS-COV-2, which causes COVID-19. Pregnant women have somewhat altered immune state, which may make them more vulnerable to COVID-19 and its complications. Extensive research is needed to better understand the clinical course of COVID-19 in this population. Objective: This review article discusses the comparison of previous coronaviruses’ outbreaks, clinical presentations, and complications in pregnant women and newborns. Study Design: We conducted literature search for case series and case reports about pregnancy outcomes in pregnant women with COVID-19 during the early phase of pandemic. Results: In case series, 37 of 129(28.6%) pregnant women with COVID-19 disease had preterm delivery and 14 of 67 pregnant women had fetal distress. The rate of preterm labor in normal pregnant women who are healthy and not infected with any virus worldwide is approximately 11%. Conclusion: Based on the articles reviewed, preterm delivery appears to be the most common complication in COVID-19 pregnant patients. Other complications include fetal distress, stillbirth, ICU admission and severe disease leading to fetal demise and maternal mortality. Pregnancy outcomes seem to be better with Covid-19 compared to SARs and MERS. However, most of these publications are from the early part of the pandemic when protocols for care for pregnant women were being worked out and comprehensive knowledge of the disease process in pregnant women was still in developing stage

The RNA helicase database

RNA helicases are ubiquitous and essential enzymes that function in nearly all aspects of RNA metabolism. The RNA helicase database (www.rnahelicase.org) integrates the wealth of accumulating information on RNA helicases in a readily accessible format. The database is a portal that allows straightforward retrieval of comprehensive information on sequence, structure and on biochemical and cellular functions of all RNA helicases from the most widely used model organisms Escherichia coli, Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, mouse and human. Also included are RNA helicases from other organisms that are subject to specific investigation. The database is structured according to the most recent helicase classification into helicase superfamilies (SFs) and families, and thus emphasizes phyologenetic relations between RNA helicases as well. Information on individual RNA helicases can be accessed through various browsing routes or through text-based searches of the database

Assessment of an International Virtual Exchange Project with Civil Engineering Students from the US and Palestine: Global Competencies, Perceived Value, and Teamwork

This paper presents the results of a study conducted to assess the value of two iterations of an international virtual exchange (IVE) experience between universities in the US (Clemson University and Bucknell University) and Palestine (An-Najah National University) in 2021 and 2022. The focus of this study was a five-week collaborative project where civil engineering students enrolled in pavement design or environmental engineering courses at three universities were tasked to develop innovative solutions to a pavement related problem in one of five general areas. Based on the course enrollments at each institution (i.e., 50 US and 19 Palestinian students in 2021 and 35 US and 51 Palestinian students in 2022), there were two treatment groups: IVE and non-IVE. In 2021 there were nine bi-national IVE teams and eight non-IVE teams composed only of students from Clemson University (US). In 2022, there were nine bi-national IVE teams, five US non-IVE teams from Clemson, and seven Palestinian non-IVE teams from An-Najah. The evaluation in this study focused on (1) global competencies, (2) value of the experience, and (3) team dynamics. The influence of the experience on the global competencies of the students in IVE and non-IVE teams was assessed quantitatively and qualitatively using pre- and post-program surveys based on the Stevens Initiative and RTI International’s Common Survey Items as well as survey items developed for this IVE to measure whether the program promotes gender equity. The value of the project experience for all students (i.e., IVE and non-IVE) was evaluated using a mixed methods assessment based on the “value-creation framework” of Wenger-Trayner et al. Four cycles of the value-creation framework were included in this assessment: (1) immediate value, (2) potential value, (3) applied value, and (4) realized value. Finally, teamwork was evaluated using the Individual and Team Performance (ITP) Metrics Peer Feedback and Team Dynamics survey. Results showed that modifications made between the first and second project iterations, specifically cross-cultural dialogue modules, had positive impacts on the overall outcomes. The IVE teams exhibited greater improvement in team dynamics measures over the project duration compared to the non-IVE teams. The students on IVE teams also showed greater gains in all aspects of the global competencies assessment than their non-IVE peers. Finally, all students expressed that they found value in the experience. However, there were no differences in perceived value between the IVE and non-IVE teams. The differences came from students from different countries as the Palestinian students perceived greater value in the experience than their US peers regardless of whether they were on an IVE team or not

Effect of the type of urinary incontinence in the Outcome of laser Therapy for Treatment of Female Stress Urinary Incontinence

Background:Female stress urinary incontinence (SUI) is common lower urinary tract symptom. It affect many women especially middle and elderlywomen and about 40% of them have mixed Urinary incontinence (MUI).There are surgical and non-surgical options for treatment of stress urinary incontinence. Laser therapy is seemed to be a promising minimally invasive option for treatment of female SUI. Objective:To determine the effect of the type of urinary incontinence in laser therapy outcomes. Patients and method(s): A prospective non-randomized study for female patients with SUI who were referred to the Urology Department, Sohag University Hospital from March 2017 to June 2018. The study was approved from Sohag University ethics committee. Patients were treated by the clinical protocol for SUI with non-ablative 2940 nm Er: YAG laser. Patients were evaluated at the baseline and every 3 months for 1 year by Validated International Consultation on Incontinence Questionnaire – Urinary Incontinence Short Form (ICIQ-UI SF) Arabic version and the number of daily pads usage .The patients were classified according to their type into a pure SUI group and MUI group. Result(s):Our results included 32female patients. 20 patients (63 %) had pure SUI while 12 patients (37%) had mixed incontinence. the median duration of symptoms 3 years. There was a significant reduction in daily pads usage and ICIQ-UISF score (p value<0001) for both groups with more significant for pure SUI group. No serious side effects were reported. Conclusion:There was a statistically significant effect of the type of UI on laser therapy outcomes with better results related to pure SUI.On the other hand, No effect of type ofurinary incontinence on number of laser sessions needed by patients to achieve improvement of their incontinence

Nonalcoholic Fatty Liver Disease and the Risk of Atrial Fibrillation

BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) is now the factor behind the development of liver cirrhosis, liver cell failure, and liver transplantation in many cases. However, its relation to atrial fibrillation (AF) could not be cleared up. AIM: The purpose of the study was to evaluate prevalence of AF in the setting of NAFLD; the association between them, and to evaluate risk factors of AF in this category of patients. METHODS: This cross-sectional study was performed on 400 patients between January 2018 and June 2019. These patients were analyzed for the presence of NAFLD and presence of persistent or chronic AF. RESULTS: There were 138 patients with NAFLD, and 20 patients with persistent or permanent AF. Factors associated with AF were old age, male gender, and high values of aspartate aminotransferase, alanine-aminotransferase, γ-glutamyltranspeptidase, and serum uric acid. The participants with AF had a significantly greater prevalence of NAFLD than those without AF. CONCLUSION: Incidence and prevalence of atrial fibrillation in NAFLD patients were high. Severity of liver disease was an important predictor of new-onset atrial fibrillation

Signatures of Selection in Fusion Transcripts Resulting From Chromosomal Translocations in Human Cancer

BACKGROUND: The recurrence and non-random distribution of translocation breakpoints in human tumors are usually attributed to local sequence features present in the vicinity of the breakpoints. However, it has also been suggested that functional constraints might contribute to delimit the position of translocation breakpoints within the genes involved, but a quantitative analysis of such contribution has been lacking. METHODOLOGY: We have analyzed two well-known signatures of functional selection, such as reading-frame compatibility and non-random combinations of protein domains, on an extensive dataset of fusion proteins resulting from chromosomal translocations in cancer. CONCLUSIONS: Our data provide strong experimental support for the concept that the position of translocation breakpoints in the genome of cancer cells is determined, to a large extent, by the need to combine certain protein domains and to keep an intact reading frame in fusion transcripts. Additionally, the information that we have assembled affords a global view of the oncogenic mechanisms and domain architectures that are used by fusion proteins. This can be used to assess the functional impact of novel chromosomal translocations and to predict the position of breakpoints in the genes involved

Comparative Structural Analysis of Human DEAD-Box RNA Helicases

DEAD-box RNA helicases play various, often critical, roles in all processes where RNAs are involved. Members of this family of proteins are linked to human disease, including cancer and viral infections. DEAD-box proteins contain two conserved domains that both contribute to RNA and ATP binding. Despite recent advances the molecular details of how these enzymes convert chemical energy into RNA remodeling is unknown. We present crystal structures of the isolated DEAD-domains of human DDX2A/eIF4A1, DDX2B/eIF4A2, DDX5, DDX10/DBP4, DDX18/myc-regulated DEAD-box protein, DDX20, DDX47, DDX52/ROK1, and DDX53/CAGE, and of the helicase domains of DDX25 and DDX41. Together with prior knowledge this enables a family-wide comparative structural analysis. We propose a general mechanism for opening of the RNA binding site. This analysis also provides insights into the diversity of DExD/H- proteins, with implications for understanding the functions of individual family members

Vasa-Like DEAD-Box RNA Helicases of Schistosoma mansoni

Genome sequences are available for the human blood flukes, Schistosoma japonicum, S. mansoni and S. haematobium. Functional genomic approaches could aid in identifying the role and importance of these newly described schistosome genes. Transgenesis is established for functional genomics in model species, which can lead to gain- or loss-of-functions, facilitate vector-based RNA interference, and represents an effective forward genetics tool for insertional mutagenesis screens. Progress toward routine transgenesis in schistosomes might be expedited if germ cells could be reliably localized in cultured schistosomes. Vasa, a member of the ATP-dependent DEAD-box RNA helicase family, is a prototypic marker of primordial germ cells and the germ line in the Metazoa. Using bioinformatics, 33 putative DEAD-box RNA helicases exhibiting conserved motifs that characterize helicases of this family were identified in the S. mansoni genome. Moreover, three of the helicases exhibited vasa-like sequences; phylogenetic analysis confirmed the three vasa-like genes—termed Smvlg1, Smvlg2, and Smvlg3—were members of the Vasa/PL10 DEAD-box subfamily. Transcripts encoding Smvlg1, Smvlg2, and Smvlg3 were cloned from cDNAs from mixed sex adult worms, and quantitative real time PCR revealed their presence in developmental stages of S. mansoni with elevated expression in sporocysts, adult females, eggs, and miracidia, with strikingly high expression in the undeveloped egg. Whole mount in situ hybridization (WISH) analysis revealed that Smvlg1, Smvlg2 and Smvlg3 were transcribed in the posterior ovary where the oocytes mature. Germ cell specific expression of schistosome vasa-like genes should provide an informative landmark for germ line transgenesis of schistosomes, etiologic agents of major neglected tropical diseases