Can sleep and resting behaviours be used as indicators of welfare in shelter dogs (Canis lupusfamiliaris)?

Previous research on humans and animals suggests that the analysis of sleep patterns may reliably inform us about welfare status, but little research of this kind has been carried out for non-human animals in an applied context. This study explored the use of sleep and resting behaviour as indicators of welfare by describing the activity patterns of dogs (Canis lupus familiaris) housed in rescue shelters, and comparing their sleep patterns to other behavioural and cognitive measures of welfare. Sleep and activity patterns were observed over five non-consecutive days in a population of 15 dogs. Subsequently, the characteristics of sleep and resting behaviour were described and the impact of activity on patterns of sleep and resting behaviour analysed. Shelter dogs slept for 2.8% of the day, 14.3% less than previously reported and experienced less sleep fragmentation at night (32 sleep bouts). There were no statistically significant relationships between behaviours exhibited during the day and sleep behaviour. A higher proportion of daytime resting behaviour was significantly associated with a positive judgement bias, less repetitive behaviour and increased time spent coded as ‘relaxed’ across days by shelter staff. These results suggest that, in the context of a busy shelter environment, the ability to rest more during the day could be a sign of improved welfare. Considering the non-linear relationship between sleep and welfare in humans, the relationship between sleep and behavioural indicators of welfare, including judgement bias, in shelter dogs may be more complex than this study could detect

Effect of light colour temperature on expression and serum profile of selected immune markers in layers

ΔΕΝ ΔΙΑΤΙΘΕΤΑΙ ΠΕΡΙΛΗΨΗThis study was carried out to quantify the effects of different temperatures of light colour on expression and serum profile of selected immune markers in Fayoumi layers. A total of 165 Fayoumi healthy pullets, 17 weeks of age were used. At laying, birds were separated in well ventilated environmentally-controlled rooms and allocated into three groups of 55 birds each (5 males and 50 females) for 3 months and these birds represented the base generation (F0). Fertile eggs were collected and the newly hatched chicks were also divided into three groups from first day of life till 3 months after laying and these represented the first generation (F1). In the two generations, the first group (control) was exposed to cool white LED light (day light) (6500 kelvin), the second group was exposed to very cool white LED light (sky blue light) (10000 kelvin) and the third group was exposed to warm white LED light (yellow light) (2700 kelvin). Birds of each group of the two generations were evaluated for expression profile of TLR4 and IL10 genes and serum level of IL10. Results showed that blue light-exposed groups, in the two generations, exhibited a higher up-regulation of TLR4 and IL10 genes and increased serum level of IL10 compared to groups experienced either white or yellow light colour. Comparison between F0 and F1 individuals revealed improved genetic profiles for F1 birds. The results therefore elucidate the benefits of using blue light in improving the immune status of layers in order to predict the most susceptible risk time for disease incidence and to build up an effective management regimen

STUDIES ON BACTERIAL POLLUTION IN LAKE MANZALA

Lake Manzala in Egypt have shown great changes in water properties including pH (7.4 to 8.4), total suspended solids (19 to 350 mg/l), total dissolved salts (1578 to 33480 mg/l), dissolved oxygen (2.25 to 8.45 mg/l), ammonia (0.00 to 44.8 mg/l) and nitrate (0.00 to 8.96 mg/l). In addition there has been quite high bacterial density in water and fish of the lake, including total viable bacterial counts and indicator organisms such as total coliforms, faecal coliforms and some other organisms such as Aeromonas spp., Vibrio spp. and Staphylococcus spp. However, the magnitude number of bacteria in water and fish was found to vary sometimes considerably and sometimes slightly depending on the site of sampling. Our results herein showed that LakeManzala is highly contaminated

Multivariable regression analysis in Schistosoma mansoni-infected individuals in the Sudan reveals unique immunoepidemiological profiles in uninfected, egg+ and non-egg+ infected individuals

Background: In the Sudan, Schistosoma mansoni infections are a major cause of morbidity in schoolaged children and infection rates are associated with available clean water sources. During infection, immune responses pass through a Th1 followed by Th2 and Treg phases and patterns can relate to different stages of infection or immunity. Methodology: This retrospective study evaluated immunoepidemiological aspects in 234 individuals(range 4–85 years old) from Kassala and Khartoum states in 2011. Systemic immune profiles(cytokines and immunoglobulins) and epidemiological parameters were surveyed in n = 110 persons presenting patent S. mansoni infections (egg+), n = 63 individuals positive for S. mansoni via PCR in sera but egg negative (SmPCR+) and n = 61 people who were infection-free (Sm uninf). Immunoepidemiological findings were further investigated using two binary multivariable regression analysis. Principal Findings: Nearly all egg+ individuals had no access to latrines and over 90% obtained water via the canal stemming from the Atbara River. With regards to age, infection and an egg+ status was linked to young and adolescent groups. In terms of immunology, S. mansoni infection per se was strongly associated with increased SEA-specific IgG4 but not IgE levels. IL-6, IL-13 and IL-10 were significantly elevated in patently-infected individuals and positively correlated with egg load. In contrast, IL-2 and IL-1β were significantly lower in SmPCR+ individuals when compared to Sm uninf and egg+ groups which was further confirmed during multivariate regression analysis. Conclusions/Significance: Schistosomiasis remains an important public health problem in the Sudan with a high number of patent individuals. In addition, SmPCR diagnostics revealed another cohort of infected individuals with a unique immunological profile and provides an avenue for future studies on non-patent infection states. Future studies should investigate the downstream signalling pathways/mechanisms of IL-2 and IL-1β as potential diagnostic markers in order to distinguish patent from non-patent individuals

Why Are Outcomes Different for Registry Patients Enrolled Prospectively and Retrospectively? Insights from the Global Anticoagulant Registry in the FIELD-Atrial Fibrillation (GARFIELD-AF).

Background: Retrospective and prospective observational studies are designed to reflect real-world evidence on clinical practice, but can yield conflicting results. The GARFIELD-AF Registry includes both methods of enrolment and allows analysis of differences in patient characteristics and outcomes that may result. Methods and Results: Patients with atrial fibrillation (AF) and ≥1 risk factor for stroke at diagnosis of AF were recruited either retrospectively (n = 5069) or prospectively (n = 5501) from 19 countries and then followed prospectively. The retrospectively enrolled cohort comprised patients with established AF (for a least 6, and up to 24 months before enrolment), who were identified retrospectively (and baseline and partial follow-up data were collected from the emedical records) and then followed prospectively between 0-18 months (such that the total time of follow-up was 24 months; data collection Dec-2009 and Oct-2010). In the prospectively enrolled cohort, patients with newly diagnosed AF (≤6 weeks after diagnosis) were recruited between Mar-2010 and Oct-2011 and were followed for 24 months after enrolment. Differences between the cohorts were observed in clinical characteristics, including type of AF, stroke prevention strategies, and event rates. More patients in the retrospectively identified cohort received vitamin K antagonists (62.1% vs. 53.2%) and fewer received non-vitamin K oral anticoagulants (1.8% vs . 4.2%). All-cause mortality rates per 100 person-years during the prospective follow-up (starting the first study visit up to 1 year) were significantly lower in the retrospective than prospectively identified cohort (3.04 [95% CI 2.51 to 3.67] vs . 4.05 [95% CI 3.53 to 4.63]; p = 0.016). Conclusions: Interpretations of data from registries that aim to evaluate the characteristics and outcomes of patients with AF must take account of differences in registry design and the impact of recall bias and survivorship bias that is incurred with retrospective enrolment. Clinical Trial Registration: - URL: http://www.clinicaltrials.gov . Unique identifier for GARFIELD-AF (NCT01090362)

Simplified modeling of the electrospinning process from the stable jet region to the unstable region for predicting the final nanofiber diameter

Electrospinning allows the production of ultrafine nanofibers through the stretching of a charged polymer jet with an external electrostatic field. In this study, we derived a simplified and accurate model relating the processing parameters, including the solution volumetric flow rate (Q), the applied electric field (E), and the polymer concentration, to the final fiber diameter. The model takes into consideration the jet behavior starting at the stable region and moving to the bending instability region. We validated the model experimentally by performing the electrospinning process with a polyacrylonitrile/N,N-dimethylformamide solution with different ranges of concentrations (8–11 wt %), Qs (900–1320 μL/h), and Es (88,889–113,889 V/m). The final fiber diameter was measured with scanning electron microscopy. The model predicted the fiber diameter with a relative error of less than 10%. Moreover, a 30% increase in Q resulted in a 15% increase in the fiber diameter, whereas a 30% increase in E resulted in a 14% decrease in the fiber diameter. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 44112. © 2016 Wiley Periodicals, Inc

Electrospun waterproof breathable membrane with a high level of aerosol filtration

In this study, several nanofibrous polyurethane (PU) webs were electrospun (ES) by changing different effective parameters (e.g., polymeric concentration, voltage, feed flow, etc.). The physical–chemical properties of the webs (i.e., average fiber diameters, thickness, areal density, porosity, contact angle, waterproofness, air permeability (AP), water vapor transmittance, and aerosol filtration) were studied based on the standard test methods. A commercially available waterproof breathable (WPB) fabric was used as a reference for benchmarking. The beads-free webs with an average fiber diameter as small as 200 nm were achieved from electrospinning of 10 wt % PU in N,N-dimethylformamide, at feed rate of 0.5 mL/h, applied voltage of 25 kV, and tip-to-collector distance of 15 cm. By optimizing the electrospinning parameters, a web with a high level of waterproofness, high AP, and high water vapor transmission rate (WVTR) was obtained. In addition, the selected ES membrane showed very promising aerosol filtration efficiency with complete removal of particles larger than 0.5 µm, and 94% reduction in the concentration of smaller particles. We found a linear empirical equation for the estimation of AP and WVTR based on the average pore size diameter, the membrane thickness, and the porosity with very high regression coefficients (R2 > 0.97). © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 45660. © 2017 Wiley Periodicals, Inc