Modulations of the Chicken Cecal Microbiome and Metagenome in Response to Anticoccidial and Growth Promoter Treatment

With increasing pressures to reduce or eliminate the use of antimicrobials for growth promotion purposes in production animals, there is a growing need to better understand the effects elicited by these agents in order to identify alternative approaches that might be used to maintain animal health. Antibiotic usage at subtherapeutic levels is postulated to confer a number of modulations in the microbes within the gut that ultimately result in growth promotion and reduced occurrence of disease. This study examined the effects of the coccidiostat monensin and the growth promoters virginiamycin and tylosin on the broiler chicken cecal microbiome and metagenome. Using a longitudinal design, cecal contents of commercial chickens were extracted and examined using 16S rRNA and total DNA shotgun metagenomic pyrosequencing. A number of genus-level enrichments and depletions were observed in response to monensin alone, or monensin in combination with virginiamycin or tylosin. Of note, monensin effects included depletions of Roseburia, Lactobacillus and Enterococcus, and enrichments in Coprococcus and Anaerofilum. The most notable effect observed in the monensin/virginiamycin and monensin/tylosin treatments, but not in the monensin-alone treatments, was enrichments in Escherichia coli. Analysis of the metagenomic dataset identified enrichments in transport system genes, type I fimbrial genes, and type IV conjugative secretion system genes. No significant differences were observed with regard to antimicrobial resistance gene counts. Overall, this study provides a more comprehensive glimpse of the chicken cecum microbial community, the modulations of this community in response to growth promoters, and targets for future efforts to mimic these effects using alternative approaches

Satisfaction with Life after Mild Traumatic Brain Injury: A TRACK-TBI Study

Identifying the principal determinants of life satisfaction following mild TBI (mTBI) may inform efforts to improve subjective well-being in this population. We examined life satisfaction among participants in the Transforming Research and Clinical Knowledge in Traumatic Brain Injury (TRACK-TBI) study who presented with mTBI (Glasgow Coma Scale [GCS] score = 13-15; n = 1152). An L1-regularization path algorithm was used to select optimal sets of baseline and concurrent symptom measures for prediction of scores on the Satisfaction with Life Scale (SWLS) at 2 weeks and 3, 6, and 12 months post-injury. Multi-variable linear regression models (all n = 744-894) were then fit to evaluate associations between the empirically selected predictors and SWLS scores at each follow-up visit. Results indicated that emotional post-TBI symptoms (all b = -1.27 to -0.77, all p < 0.05), anhedonia (all b = -1.59 to -1.08, all p < 0.01), and pain interference (all b = -1.38 to -0.89, all p < 0.001) contributed to the prediction of lower SWLS scores at all follow-ups. Insomnia predicted lower SWLS scores at 2 weeks, 3 months, and 6 months (all b = -1.11 to -0.83, all ps < 0.01); and negative affect predicted lower SWLS scores at 2 weeks, 3 months, and 12 months (all b = -1.38 to -0.80, all p < 0.005). Other post-TBI symptom domains and baseline socio-demographic, injury-related, and clinical characteristics did not emerge as robust predictors of SWLS scores during the year after mTBI. Efforts to improve satisfaction with life following mTBI may benefit from a focus on the detection and treatment of affective symptoms, pain, and insomnia. The results reinforce the need for tailoring of evidence-based treatments for these conditions to maximize efficacy in patients with mTBI

Conversion of Reactance Motives Into Effortful Goal Pursuit

Abstract. As originally formulated, Brehm’s theory of psychological reactance posited two manifestations of reactance, (1) an increased desire to exercise the threaten freedom, and (2) an increased tendency to exercise that freedom. The theory included no statement about what determines the intensity of reactant striving, although many have assumed that striving intensity should be proportional to the magnitude of reactance, that is, the strength of the reactance motive. Conceptual links between Brehm’s reactance theory and his later theory of motivation intensity have rarely been made. However, the motivation intensity theory in fact has important implications for how reactance motives should convert into effortful goal pursuit. This article identifies central motivation intensity theory implications and discusses in summary a body of evidence that bears them out, albeit indirectly. The broad indication is that reactance should increase the willingness to deploy effort in the interest of restoring or confirming freedoms, with the conversion of willingness into action depending on what can, will, and must be done to achieve these ends. </jats:p

Investigation of the Association between Recreational Scuba Diving and a Case of Acute Lemierre’s Syndrome: Will it Recur?

Lemierre’s Syndrome (LS) is a septic thrombophlebitis of the internal jugular vein following acute pharyngitis commonly caused by anaerobic bacteria. We postulate a rare source of infection in a young male never before cited in the literature, scuba diving. We discuss the patient’s risk factors which we postulate likely caused an altered innate immunity, and when combined with superimposed barotrauma, likely provoked the infection. We then consider the possibility of disease recurrence and make certain recommendations for prevention. Additionally, we highlight the importance of early diagnosis in potentially critical diseases such as LS.</jats:p