Bradford Multi-Modal Gait Database: Gateway to Using Static Measurements to Create a Dynamic Gait Signature

YesAims: To create a gait database with optimum accuracy of joint rotational data and an accu-rate representation of 3D volume, and explore the potential of using the database in studying the relationship between static and dynamic features of a human’s gait. Study Design: The study collected gait samples from 38 subjects, in which they were asked to walk, run, walk to run transition, and walk with a bag. The motion capture, video, and 3d measurement data extracted was used to analyse and build a correlation between features. Place and Duration of Study: The study was conducted in the University of Bradford. With the ethical approval from the University, 38 subjects’ motion and body volumes were recorded at the motion capture studio from May 2011- February 2013. Methodology: To date, the database includes 38 subjects (5 females, 33 males) conducting walk cycles with speed and load as covariants. A correlation analysis was conducted to ex-plore the potential of using the database to study the relationship between static and dynamic features. The volumes and surface area of body segments were used as static features. Phased-weighted magnitudes extracted through a Fourier transform of the rotation temporal data of the joints from the motion capture were used as dynamic features. The Pearson correlation coefficient is used to evaluate the relationship between the two sets of data. Results: A new database was created with 38 subjects conducting four forms of gait (walk, run, walk to run, and walking with a hand bag). Each subject recording included a total of 8 samples of each form of gait, and a 3D point cloud (representing the 3D volume of the subject). Using a Pvalue (P<.05) as a criterion for statistical significance, 386 pairs of features displayed a strong relationship. Conclusion: A novel database available to the scientific community has been created. The database can be used as an ideal benchmark to apply gait recognition techniques, and based on the correlation analysis, can offer a detailed perspective of the dynamics of gait and its relationship to volume. Further research in the relationship between static and dynamic features can contribute to the field of biomechanical analysis, use of biometrics in forensic applications, and 3D virtual walk simulation

An outline on the global insights of implementation and challenges in primary healthcare telemedicine

Background: The utilization of telephone consultation (TC) has seen a significant increase of up to 86% since the start of the COVID-19 pandemic. In order to bridge the existing knowledge gap by examining the usage and efficacy of telephone or virtual consultations in a clinical setting during the COVID-19 lockdown, the objective of the current review is to gain a comprehensive understanding of the advantages and limitations of TC in the light of physicians and familial perspectives to enlighten future healthcare planning and decision-making. Methods: The data utilized in this research spanning the period of COVID-19 and other studies related to TC that occurred before the onset of the pandemic were gathered from a variety of reputable sources, such as Pubmed, Pubmed Central, Google Scholar, Research Gate, and Science Direct with the pre-established eligibility criteria and relevant keywords. Results: Studies revealed that the incorporation of teleconsultation has demonstrated numerous benefits for patients, including effective handling of data, fair accessibility, and adherence to standardized care protocols. Nonetheless, the implementation of TC also presents obstacles such as insufficiently trained staff, technical hurdles like connectivity issues and unreliable internet connections, and the possibility of erroneous diagnoses. Conclusions: During the COVID-19 phase, TC has proven effective with fewer limitations that can be minimized by training the healthcare staff and overcoming technical issues

Activation-induced thrombospondin-4 works with thrombospondin-1 to build cytotoxic supramolecular attack particles

Cytotoxic attack particles released by CTLs and NK cells include diverse phospholipid membrane and glycoprotein encapsulated entities that contribute to target cell killing. Supramolecular attack particles (SMAPs) are one type of particle characterized by a cytotoxic core enriched in granzymes and perforin surrounded by a proteinaceous shell including thrombospondin (TSP)-1. TSP-4 was also detected in bulk analysis of SMAPs released by CTLs; however, it has not been investigated whether TSP-4 contributes to distinct SMAP types or the same SMAP type as TSP-1 and, if in the same type of SMAP, whether TSP-4 and TSP-1 cooperate or compete. Here, we observed that TSP-4 expression increased upon CD8+ T cell activation while, surprisingly, TSP-1 was down-regulated. Correlative Light and Electron Microscopy and Stimulated Emission Depletion microscopy localized TSP-4 and TSP-1 in SMAP-containing multicore granules. Superresolution dSTORM revealed that TSP-4 and TSP-1 are usually enriched in the same SMAPs while particles with single-positive shells are rare. Retention Using Selective Hooks assays showed that TSP-4 localizes to the lytic granules faster than TSP-1 and promotes its accumulation therein. TSP-4 contributed to direct CTL-mediated killing, as previously shown for TSP-1. TSP-4 and TSP-1 were both required for latent SMAP-mediated cell killing, in which released SMAPs kill targets after removal of the CTLs. Of note, we found that chronic lymphocytic leukemia (CLL) cell culture supernatants suppressed expression of TSP-4 in CTL and latent SMAP-mediated killing. These results identify TSP-4 as a functionally important component of SMAPs and suggest that SMAPs may be targeted for immune suppression by CLL

One-step nanoscale expansion microscopy reveals individual protein shapes

The attainable resolution of fluorescence microscopy has reached the subnanometer range, but this technique still fails to image the morphology of single proteins or small molecular complexes. Here, we expand the specimens at least tenfold, label them with conventional fluorophores and image them with conventional light microscopes, acquiring videos in which we analyze fluorescence fluctuations. One-step nanoscale expansion (ONE) microscopy enables the visualization of the shapes of individual membrane and soluble proteins, achieving around 1-nm resolution. We show that conformational changes are readily observable, such as those undergone by the ~17-kDa protein calmodulin upon Ca2+ binding. ONE is also applied to clinical samples, analyzing the morphology of protein aggregates in cerebrospinal fluid from persons with Parkinson disease, potentially aiding disease diagnosis. This technology bridges the gap between high-resolution structural biology techniques and light microscopy, providing new avenues for discoveries in biology and medicine

One-step nanoscale expansion microscopy reveals individual protein shapes

\ua9 The Author(s) 2024.The attainable resolution of fluorescence microscopy has reached the subnanometer range, but this technique still fails to image the morphology of single proteins or small molecular complexes. Here, we expand the specimens at least tenfold, label them with conventional fluorophores and image them with conventional light microscopes, acquiring videos in which we analyze fluorescence fluctuations. One-step nanoscale expansion (ONE) microscopy enables the visualization of the shapes of individual membrane and soluble proteins, achieving around 1-nm resolution. We show that conformational changes are readily observable, such as those undergone by the ~17-kDa protein calmodulin upon Ca2+ binding. ONE is also applied to clinical samples, analyzing the morphology of protein aggregates in cerebrospinal fluid from persons with Parkinson disease, potentially aiding disease diagnosis. This technology bridges the gap between high-resolution structural biology techniques and light microscopy, providing new avenues for discoveries in biology and medicine

Characterization of Two Types of Stainless Steels Recommended for Manufacturing Brine Recirculation Pumps

In earlier works, characterization and stress corrosion cracking of casings of brine recirculation pumps, used in desalination plants, had been investigated. These casings which were manufactured from two types of Ni resist ductile irons have been reported to show different service lives. Material selection of casings is believed to be one of possible factors to extend the service life of these pumps. Two types of stainless steels; UNS S31603 and UNS S32750 have been recommended as substitutes to Ni resist ductile irons. In this work, mechanical, metallurgical, and electrochemical tests have been conducted on as received samples, made of these two types of stainless steels. Results have shown considerable higher yield and tensile strengths and corrosion resistance for the UNS S32750 over the UNS S31603. Results have also shown reproduced pitting behavior illustrated by measured pitting potentials and visual observations for UNS S31603 samples. UNS S32750 samples have shown no signs of pitting.</jats:p

The relationship between 2D static features and 2D dynamic features used in gait recognition

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