Classification of coffee beans by GC-C-IRMS, GC-MS, and 1H-NMR

In a previous work using 1H-NMR we reported encouraging steps towards the construction of a robust expert system for the discrimination of coffees from Colombia versus nearby countries (Brazil and Peru), to assist the recent protected geographical indication granted to Colombian coffee in 2007.This system relies on fingerprints acquired on a 400MHz magnet and is thus well suited for small scale random screening of samples obtained at resellers or coffee shops. However, this approach cannot easily be implemented at harbour's installations, due to the elevated operational costs of cryogenic magnets. This limitation implies shipping the samples to the NMR laboratory, making the overall approach slower and thereby more expensive and less attractive for large scale screening at harbours. In this work, we report on our attempt to obtain comparable classification results using alternative techniques that have been reported promising as an alternative toNMR: GC-MS andGC-C-IRMS.Although statistically significant information could be obtained by all threemethods, the results showthat the quality of the classifiers dependsmainly on the number of variables included in the analysis; hence NMR provides an advantage since more molecules are detected to obtain a model with better predictions

What Matters Most to Patients and Rheumatologists? A Discrete Choice Experiment in Rheumatoid Arthritis

Introduction: To determine patient and rheumatologist preferences for rheumatoid arthritis (RA) treatment attributes in Spain and to evaluate their attitude towards shared decision-making (SDM). Methods: Observational, descriptive, exploratory and cross-sectional study based on a discrete choice experiment (DCE). To identify the attributes and their levels, a literature review and two focus groups (patients [P] = 5; rheumatologists [R] = 4) were undertaken. Seven attributes with 2–4 levels were presented in eight scenarios. Attribute utility and relative importance (RI) were assessed using a conditional logit model. Patient preferences for SDM were assessed using an ad hoc questionnaire. Results: Ninety rheumatologists [52.2% women; mean years of experience 18.1 (SD: 9.0); seeing an average of 24.4 RA patients/week (SD: 15.3)] and 137 RA patients [mean age: 47.5 years (SD: 10.7); 84.0% women; mean time since diagnosis of RA: 14.2 years (SD: 11.8) and time in treatment: 13.2 years (SD: 11.2), mean HAQ score 1.2 (SD: 0.7)] participated in the study. In terms of RI, rheumatologists and RA patients viewed: time with optimal QoL: R: 23.41%/P: 35.05%; substantial symptom improvement: R: 13.15%/P: 3.62%; time to onset of treatment action: R: 16.24%/P: 13.56%; severe adverse events: R: 10.89%/P: 11.20%; mild adverse events: R: 4.16%/P: 0.91%; mode of administration: R: 25.23%/P: 25.00%; and added cost: R: 6.93%/P: 10.66%. Nearly 73% of RA patients were involved in treatment decision-making to a greater or lesser extent; however, 27.4% did not participate at all. Conclusion: Both for rheumatologists and patients, the top three decision-making drivers are time with optimal quality, treatment mode of administration and time to onset of action, although in different ranking order. Patients were willing to be more involved in the treatment decision-making process

An effort to make sense of antisense transcription in bacteria

Analysis of bacterial transcriptomes have shown the existence of a genome-wide process of overlapping transcription due to the presence of antisense RNAs, as well as mRNAs that overlapped in their entire length or in some portion of the 5'- and 3'-UTR regions. The biological advantages of such overlapping transcription are unclear but may play important regulatory roles at the level of transcription, RNA stability and translation. In a recent report, the human pathogen Staphylococcus aureus is observed to generate genome-wide overlapping transcription in the same bacterial cells leading to a collection of short RNA fragments generated by the endoribonuclease III, RNase III. This processing appears most prominently in Gram-positive bacteria. The implications of both the use of pervasive overlapping transcription and the processing of these double stranded templates into short RNAs are explored and the consequences discussed

An analysis of the variables that provide a supply chain with sustainable competitiveness

Objeto: Una cadena de suministro (CS) ágil, adaptable y alineada (Triple A) parece clave para la obtención de ventajas competitivas sostenibles. Sin embargo, hay escasa investigación previa sobre el tema, observándose incluso discrepancias a nivel conceptual. Por ello, el objetivo del presente trabajo es proponer un marco de referencia que determine las dimensiones y factores que definen la agilidad, adaptabilidad y alineación en la CS y facilite a los gestores e investigadores la evaluación del estado de la misma en lo que respecta a estas variables, así como el desarrollo de investigaciones empíricas que determinen su impacto en el rendimiento. Diseño/metodología/enfoque: Se realiza una revisión sistemática de bibliografía en revistas especializadas en Dirección de Operaciones, Logística, Administración y Gestión de la Cadena de Suministro utilizando la base de datos ABI/Inform. Los artículos obtenidos fueron revisados, seleccionándose los relevantes para este estudio. Con ellos se realizó un análisis cualitativo que nos llevó a la consecución del objetivo planteado. Aportaciones y resultados: En la presente investigación se establecen y agrupan, por primera vez, las definiciones, dimensiones y factores de la triple A en la CS, ofreciendo un marco conceptual que apoyará a investigadores y gestores a evaluar el desempeño de la CS. Aunque los artículos que analizan alguna de estas variables van en aumento, siguen siendo escasos. La agilidad es la variable más estudiada, siendo la adaptabilidad la menos analizada. Limitaciones / implicaciones: Se propone un marco de referencia teórico para la triple A en la CS construido a partir de estudios previos que no evalúan de manera conjunta las tres variables. El modelo será analizado empíricamente en futuras investigaciones. Originalidad / Valor añadido: La escasez de trabajos sobre agilidad, adaptabilidad y alineación en la CS, así como la falta de consenso respecto a las dimensiones y factores que las definen, hacen necesarios estudios como el que se presenta.Purpose: An agile, adaptable and aligned (Triple A) supply chain (SC) would seem to be key to obtaining sustainable competitive advantages. Little previous research has been done into the topic, however, and there are even discrepancies on the conceptual level. For this reason this study aims to propose a reference framework to determine the dimensions and factors that define agility, adaptability and alignment in the SC and to facilitate both the evaluation of its state by managers and researchers with respect to these variables and also the development of empirical research that determines its impact on performance. Design/methodology: A systematic literature review was carried out of specialist Operations Management, Logistics, Management and Supply Chain Management journals using the ProQuest (Abi/Inform Global) database. The articles retrieved were examined and those that were relevant for this study were selected. Using these, a qualitative analysis was done that led to the proposed goal being achieved. Findings: This study sets out the definitions, dimensions and factors of the three variables and groups them together for the first time, thus providing a solid conceptual frame. Although the number of articles that analyse one or other of the variables is growing, it is still low. Agility is the variable on which most research has been done, while adaptability is the least analysed. Research limitations/implications: A theoretical reference framework is proposed for the Triple A in the SC based on earlier studies which do not discuss the joint effect of three variables, as a result of which there is no tested theoretical base. The model will be analysed empirically in future research. Originality/value: The lack of papers on agility, adaptability and alignment in the supply chain and the lack of a consensus regarding the dimensions and factors to define them reveal a need for studies such as this.Peer Reviewe

The impact of deep-sea fisheries and implementation of the UNGA Resolutions 61/105 and 64/72. Report of an international scientific workshop

The scientific workshop to review fisheries management, held in Lisbon in May 2011, brought together 22 scientists and fisheries experts from around the world to consider the United Nations General Assembly (UNGA) resolutions on high seas bottom fisheries: what progress has been made and what the outstanding issues are. This report summarises the workshop conclusions, identifying examples of good practice and making recommendations in areas where it was agreed that the current management measures fall short of their target