Globoidnan A, rabdosiin and globoidnan B as new phenolic markers in European‐sourced comfrey (Symphytum officinale L.) root samples

Introduction: Symphytum officinale L. (comfrey, Boraginaceae) is a cultivated or spontaneously growing medicinal plant that is traditionally used for the treatment of bone fractures, hematomas, muscle pains and joint pains. A wide range of topical preparations and dried roots for ex tempore applications are marketed in European drug stores or pharmacies. Objective: The aim of this study was to perform the qualitative and quantitative analysis of pyrrolizidine alkaloids (PAs) and phenolic compounds in the hydroethanolic extracts of 16 commercial comfrey root batches purchased from 12 different European countries. Methods: Liquid chromatography hyphenated with high-resolution tandem mass spectrometry (LC-HRMS/MS) was used for the profiling of PAs and phenolic compounds, whereas LC-MS/MS and liquid chromatography with diode array detection (LC-DAD) were used for their quantification. Results: 20 PAs (i.e. intermedine, lycopsamine, acetylintermedine, acetyllycopsamine, symphytine, symphytine-N-oxide), 17 phenolic compounds (i.e. caffeic and rosmarinic acids, rabdosiin, globoidnan A, globoidnan B) and 9 nonphenolic compounds (sugars, organic and fatty acids) were fully or partly annotated in the analysed samples. In addition, the quantitative analyses revealed that globoidnan B, rabdosiin and globoidnan A are new phenolic markers that can be used together with rosmarinic acid and PAs for the quality control of commercial comfrey root batches. Conclusions: This study brings new insights into the phytochemical complexity of S. officinale, revealing not only numerous toxic PAs, but also a significant number of valuable phenolic compounds that could contribute to the bioactivities of comfreybased preparation

Comparison of the predictive validity of the Braden, Munro and 3S scales in surgical patients

Aim of the study: This research is a descriptive and cross-sectional study aimed at examining the use of three different scales to assess the risk of pressure injury (PI) in surgical patients. Materials and methods: This study was conducted between February 1 and July 1, 2022. The study included patients who had planned surgery in general surgery clinic. The sample size was 388 patients. Patients who agreed to participate in the study were asked to fill out the “ Introductory Information Form ” and perform a risk assessment using the Braden, Munro and 3S Scales. The statistics program IBM SPSS Statistics 25.0 packaged software was used in the analyses of data. Results: It is evident that the relationship between the scales and the diagnosis of PI, as well as the strength of this relationship, are statistically significant (p < 0.05). The Munro Scale exhibited the highest value of Phi value and validity coefficient c, indicating a stronger association with the diagnosis of PI, thus suggesting its greater effectiveness in discrimination. According to the validity coefficients found, it can be stated that the correct classification percentages for Munro, Braden, and 3S Scales were 91 %, 71 %, and 66 %, respectively. Conclusion: The findings of this study indicate that both the Braden, Munro, and 3S Scales can be used for PI risk assessment in surgical patients, but the Munro Scale exhibits superior predictive validity compared to the Braden and 3S Scales in terms of overall sensitivity and specificity. © 2024 Tissue Viability Society / Society of Tissue Viabilit

Artificial Light-Induced Cytokine Gene Expression in Rabbit Cornea Effect of Ocular Drops Containing Flurbiprofen

The purpose of this study is to examine the effect of artificial sunlight on the gene expression of TNF-α, IL-6 and IL-8 in rabbit cornea in vivo , as well as the potential of an ocular anti-inflammatory formulation containing sodium flurbiprofen to suppress this effect. New Zealand Albino rabbits were subjected to acute exposure of their eyes to irradiation emitted from a light bulb commonly used for artificial tanning purposes with and without topical application of a commercially available formulation containing sodium flurbiprofen. Semi-quantitative RT-PCR was used as a means of estimating gene expression. The gene expression of IL-6 and IL-8 was found statistically significantly increased at 24 hrs post-exposure time (p = 0.003 and 0.006, respectively), as was that of TNF-α albeit in a non-statistically significant manner. A sodium flurbiprofen (0.03%) formulation was found to effectively reduce the light-induced upregulation of all three cytokines in the rabbit cornea