Electron scattering from molecules and molecular aggregates of biological relevance

In this Topical Review we survey the current state of the art in the study of low energy electron collisions with biologically relevant molecules and molecular clusters. We briefly describe the methods and techniques used in the investigation of these processes and summarise the results obtained so far for DNA constituents and their model compounds, amino acids, peptides and other biomolecules. The applications of the data obtained is briefly described as well as future required developments

Understanding Design Patterns Density with Aspects: A Case Study in JHotDraw using AspectJ

International audienceDesign patterns offer solutions to common engineering prob- lems in programs [1]. In particular, they shape the evolution of program elements. However, their implementations tend to vanish in the code: thus it is hard to spot them and to understand their impact. The prob- lem becomes even more difficult with a "high density of pattern": then the program becomes easy to evolve in the direction allowed by patterns but hard to change [2]. Aspect languages offer new means to modular- ize elements. Implementations of object-oriented design patterns with AspectJ have been proposed [3]. We aim at testing the scalability of such solutions in the JHotDraw framework. We first explore the impact of density on pattern implementation. We show how AspectJ helps to reduce this impact. This unveils the principles of aspects and AspectJ to control pattern density

Public opinion on energy crops in the landscape: considerations for the expansion of renewable energy from biomass

Public attitudes were assessed towards two dedicated biomass crops – Miscanthus and Short Rotation Coppice (SRC), particularly regarding their visual impacts in the landscape. Results are based on responses to photographic and computer-generated images as the crops are still relatively scarce in the landscape. A questionnaire survey indicated little public concern about potential landscape aesthetics but more concern about attendant built infrastructure. Focus group meetings and interviews indicated support for biomass end uses that bring direct benefits to local communities. Questions arise as to how well the imagery used was able to portray the true nature of these tall, dense, perennial plants but based on the responses obtained and given the caveat that there was limited personal experience of the crops, it appears unlikely that wide-scale planting of biomass crops will give rise to substantial public concern in relation to their visual impact in the landscape

Genome-wide profiling of methylation identifies novel targets with aberrant hyper-methylation and reduced expression in low-risk myelodysplastic syndromes

Gene expression profiling signatures may be used to classify the subtypes of Myelodysplastic syndrome (MDS) patients. However, there are few reports on the global methylation status in MDS. The integration of genome-wide epigenetic regulatory marks with gene expression levels would provide additional information regarding the biological differences between MDS and healthy controls. Gene expression and methylation status were measured using high-density microarrays. A total of 552 differentially methylated CpG loci were identified as being present in low-risk MDS; hypermethylated genes were more frequent than hypomethylated genes. In addition, mRNA expression profiling identified 1005 genes that significantly differed between low-risk MDS and the control group. Integrative analysis of the epigenetic and expression profiles revealed that 66.7% of the hypermethylated genes were underexpressed in low-risk MDS cases. Gene network analysis revealed molecular mechanisms associated with the low-risk MDS group, including altered apoptosis pathways. The two key apoptotic genes BCL2 and ETS1 were identified as silenced genes. In addition, the immune response and micro RNA biogenesis were affected by the hypermethylation and underexpression of IL27RA and DICER1. Our integrative analysis revealed that aberrant epigenetic regulation is a hallmark of low-risk MDS patients and could have a central role in these diseases. © 2013 Macmillan Publishers Limited All rights reserved

Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma

Background: Severe asthma in horses, known as severe equine asthma (SEA), is a prevalent, performance-limiting disease associated with increased allergen-specific immunoglobulin E (IgE) against a range of environmental aeroallergens. Objective: To develop a protein microarray platform to profile IgE against a range of proven and novel environmental proteins in SEA-affected horses. Animals: Six SEA-affected and 6 clinically healthy Warmblood performance horses. Methods: Developed a protein microarray (n = 384) using protein extracts and purified proteins from a large number of families including pollen, bacteria, fungi, and arthropods associated with the horses, environment. Conditions were optimized and assessed for printing, incubation, immunolabeling, biological fluid source, concentration techniques, reproducibility, and specificity. Results: This method identified a number of novel allergens, while also identifying an association between SEA and pollen sensitization. Immunolabeling methods confirmed the accuracy of a commercially available mouse anti-horse IgE 3H10 source (R2 = 0.91). Biological fluid source evaluation indicated that sera and bronchoalveolar lavage fluid (BALF) yielded the same specific IgE profile (average R2 = 0.75). Amicon centrifugal filters were found to be the most efficient technique for concentrating BALF for IgE analysis at 40-fold. Overnight incubation maintained the same sensitization profile while increasing sensitivity. Reproducibility was demonstrated (R2 = 0.97), as was specificity using protein inhibition assays. Arthropods, fungi, and pollens showed the greatest discrimination for SEA. Conclusions and clinical importance: We have established that protein microarrays can be used for large-scale IgE mapping of allergens associated with the environment of horses. This technology provides a sound platform for specific diagnosis, management, and treatment of SEA

Cultural Orientations of sport managers

Various interpretations of sport management are cultural constructs underpinned by core assumptions and values held by members of professional communities. Sport managers world wide share common problems, but differ in how they resolve them. These universal differences emerge from the relationships they form with other people, and their attitude to time, activities and the natural environment. This paper examines the role of sport managers’ cultural orientations in the interpretation and practice of sport management. Using a multiple dimension model (Hampden-Turner and Trompenaars, 2000) it sketches the cultural profiles of fifteen sport managers from seven countries. A combination of methods was employed including questionnaires, interviews and participant observation. It is contended that the culture of sport management concerns a social process by which managers get involved in reconciling seven fundamental cultural dilemmas in order to perform tasks and achieve certain ends. Thus, a knowledge of the cultural meaning of sport management in a particular country would equip sport managers with a valuable tool in managing both the cultural diversity of their own work forces and in developing appropriate cross-cultural skills needed for running international events, marketing campaigns, sponsorship deals and joint ventures

Clients’ Perspectives of Meaningful Healthcare Relationships

The aim of this study was to increase understanding of how clients view meaningful relationships within the healthcare context and how clients respond to differences in approaches or expectations for a meaningful relationship

THE INFLUENCE OF CHANGES IN SPRINT ABlLlN ON THE SLED VELOCIN PROFILE DURING THE SKELETON START

Sprint times are key predictors of skeleton start petformance, but the effect of enhancing sprint ability on the development of sled velocity is unknown. Twelve well-trained skeleton athletes performed three dry-land push-starts and three 30-m sprints before and after 16 training weeks. Sled velocity profiles were characterised using three descriptors (pre-load velocity, pre-load distance and load effectiveness) and a sled acceleration index was used to evaluate performance. Increases in pre-load velocity and distance were related to improved sprint times. However, enhanced sprint ability did not guarantee a faster start as reductions in load effectiveness were also observed when higher velocities were attained. Coaches could supplement physical conditioning with load technique training to potentially improve the transfer of training-induced sled velocity increases across the loading phase

The effect of altering loading distance on skeleton start performance: Is higher pre-load velocity always beneficial?

Athletes initiating skeleton runs differ in the number of steps taken before loading the sled. We aimed to understand how experimentally modifying loading distance influenced sled velocity and overall start performance. Ten athletes (five elite, five talent; 67% of all national athletes) underwent two to four sessions, consisting of two dry-land push starts in each of three conditions (preferred, long and short loading distances). A magnet encoder on the sled wheel provided velocity profiles and the overall performance measure (sled acceleration index). Longer pre load distances (12% average increase from preferred to long distances) were related to higher pre-load velocity (r = 0.94), but lower load effectiveness (r = 0.75; average reduction 29%). Performance evaluations across conditions revealed that elite athletes’ preferred distance push starts were typically superior to the other conditions. Short loading distances were generally detrimental, whereas pushing the sled further improved some talent-squad athletes’ performance. Thus, an important trade-off between generating high pre load velocity and loading effectively was revealed, which coaches should consider when encouraging athletes to load later. This novel intervention study conducted within a real-world training setting has demonstrated the scope to enhance push-start performance by altering loading distance, particularly in developing athletes with less extensive training experience