Effects of Accentuated Eccentric and Maximal Strength High-Resistance Training Programs with or Without a Curcumin-Based Formulation Supplement on Body Composition, Blood Pressure, and Metabolic Parameters in Older Adults

Background/Objectives: This study compared the effects of high-resistance training (RT) programs, with or without curcumin supplementation, on variables commonly associated with metabolic syndrome (MetS), including body composition, blood pressure, and metabolic parameters. Methods: Eighty-one older adults at risk of MetS (BMI > 25 kg/m2, fat mass > 32%, and systolic blood pressure > 130 mmHg in half of the participants) were randomly assigned to six groups, which were comprised as follows: four experimental groups involving either accentuated eccentric or maximal strength RT with curcumin or placebo and two control groups receiving either curcumin or placebo. Experimental groups completed a 16-week full-body RT with elastic bands, while controls did not exercise. Results: The results showed that (I) all experimental protocols significantly reduced fat mass (p ≤ 0.001), with the maximal strength RT group supplemented with curcumin (Max-Cur) showing the greatest reduction, at 14.3%; (II) muscle gains were significant and comparable across experimental groups (p ≤ 0.008); (III) both systolic and diastolic blood pressure decreased similarly across experimental groups (p ≤ 0.001); (IV) metabolic parameters significantly improved across experimental groups (p ≤ 0.037), except for creatinine, which showed an undesirable peak only in the Max-Cur group; (V) curcumin supplementation enhanced the effects of both RT programs; and (VI) between 54% and 100% of participants achieved clinically meaningful improvements in seven out of ten MetS-related variables across experimental groups. Conclusions: Our findings indicate that high-RT programs combined with curcumin supplementation positively impacted all MetS-related variables. The Max-Cur RT group stood out as the most beneficial, with the greatest fat mass reductions, highlighting this approach as a promising strategy to reduce the risk of MetS in older adults

Up-regulation of inducible heat shock protein-70 expression in multiple sclerosis patients

Inducible heat shock protein (HSP)70 (HSP70-1A and HSP70-1B proteins) is a chaperone responsible for assisting proper protein folding. Following stress conditions, HSP70 is highly up-regulated to mediate cytoprotective functions. In addition, HSP70 is able to trigger innate and adaptive immune responses that promote the immune recognition of antigens and to act as a cytokine when it is released. The data in the literature are controversial with regard to expression studies in peripheral blood mononuclear cells (PBMCs). In the present study, we aimed to examine if alterations of HSP70-1A/B expression are involved in the autoimmune pathogenesis of multiple sclerosis (MS). We determined both mRNA and protein expression in PBMCs of MS patients and healthy donors (HDs). We found a baseline increased expression of the HSPA1A gene in PBMCs from MS patients compared with HDs. Gene expression findings were associated with an increased protein expression of HSP70-1A/B in T lymphocytes (CD4+ and CD8+) and monocytes from MS patients under basal conditions that may reflect the immunological activation occurring in MS patients. We also provided evidence that heat shock (HS) stimulus induced HSP70-1A/B protein expression in HDs and MS patients, and that HS-induced HSP70-1A/B protein expression in monocytes correlated with the number of T2 lesions at baseline in MS patients. However, after lipopolysaccharide inflammatory stimulus, monocytes from MS patients failed to induce HSP70-1A/B protein expression. Our data hint at altered immune responses in MS and may indicate either a state of chronic stress or increased vulnerability to physiological immune responses in MS patients

The usefulness of RAPD and AFLP markers for determining genetic similarity in rye [Secale L.] species and subspecies

In this work we searched for genetic similarities in twelve wild rye species and subspecies and a control (S. cereale ssp. cereale, cv. Walet), using RAPD and AFLP markers. AFLP is useful for distinguishing homo- and heterozygotes but is not recommended for evaluation of codominant markers. We assessed the usefulness of the applied methods for examining genetic similarity in rye. RAPD yielded four groups of genetic similarity, with similarity values between 0.32 and 0.81. AFLP markers distinguished two groups of genetic similarity, ranging from 0.49 to 0.79