Un règlement religieux de la région de Larissa

Une haute stèle de marbre opisthographe trouvée il y a quelques années à Marmarini, au nord-est de Larissa (Thessalie), propose un long texte de 54 et 82 lignes aujourd’hui lisibles. En s’appuyant sur cette inscription du milieu du iie s. av. J.-C., qui d’une part comporte un calendrier cultuel et de l’autre un certain nombre de prescriptions touchant aux rites, le présent article propose une présentation de l’aspect probable du sanctuaire (péribole, temple, autel etc.), une liste des divinités concernées — Artémis Phylakè comme divinité principale, mais d’autres divinités grecques et surtout plusieurs divinités d’origine orientale jusqu’alors inconnues — et des remarques sur les modalités d’un culte qui était sans doute un culte à mystères. Ce document, par son contenu, par les détails qu’il donne, est une trouvaille exceptionnelle en Thessalie.A tall opisthographic marble stela found a few years ago at Marmarini, some kilometers north-east of Larissa, Thessaly, reveals a long inscription dating to the mid-second century BC, of which only 54 and 82 lines are legible today. Discussing the two faces of the inscription, one containing a religious calendar, the other a series of ritual prescriptions, the present paper provides an overview of the probable appearance of the sanctuary (peribolos / precint, temple, altars and equipment), a list of the deities worshipped — Artemis Phylake was the main goddess, but, alongside other Greek gods and goddesses, one also finds several deities of Eastern origin, which were previously unknown — and it also provides remarks on cult performance, which very probably included rites of initiation. This inscription, due to its length and its content, is unique in Thessaly

Purification of molybdenum oxide, growth and characterization of medium size zinc molybdate crystals for the LUMINEU program

The LUMINEU program aims at performing a pilot experiment on neutrinoless double beta decay of 100Mo using radiopure ZnMoO4 crystals operated as scintillating bolometers. Growth of high quality radiopure crystals is a complex task, since there are no commercially available molybdenum compounds with the required levels of purity and radioactive contamination. This paper discusses approaches to purify molybdenum and synthesize compound for high quality radiopure ZnMoO4 crystal growth. A combination of a double sublimation (with addition of zinc molybdate) with subsequent recrystallization in aqueous solutions (using zinc molybdate as a collector) was used. Zinc molybdate crystals up to 1.5 kg were grown by the low-thermal-gradient Czochralski technique, their optical, luminescent, diamagnetic, thermal and bolometric properties were tested.Comment: Contribution to Proc. of Int. Workshop on Radiopure Scintillators RPSCINT 2013, 17-20 September 2013, Kyiv, Ukraine; to be published in EPJ Web of Conferences; expected to be online in January 2014; 6 pages, 6 figures, and 3 table

Germ Line Origin and Somatic Mutations Determine the Target Tissues in Systemic AL-Amyloidosis

BACKGROUND: Amyloid is insoluble aggregated proteins deposited in the extra cellular space. About 25 different proteins are known to form amyloid in vivo and are associated with severe diseases such as Alzheimer's disease, prion diseases and type-2 diabetes. Light chain (AL) -amyloidosis is unique among amyloid diseases in that the fibril protein, a monoclonal immunoglobulin light chain, varies between individuals and that no two AL-proteins with identical primary structures have been described to date. The variability in tissue distribution of amyloid deposits is considerably larger in systemic AL-amyloidosis than in any other form of amyloidosis. The reason for this variation is believed to be based on the differences in properties of the amyloidogenic immunoglobulin light chain. However, there is presently no known relationship between the structure of an AL-protein and tissue distribution. METHODOLOGY/PRINCIPAL FINDINGS: We compared the pattern of amyloid deposition in four individuals with amyloid protein derived from variable light chain gene O18-O8, the source of a high proportion of amyloidogenic light chains, and in whom all or most of the fibril protein had been determined by amino acid sequencing. In spite of great similarities between the structures of the proteins, there was a pronounced variability in deposition pattern. We also compared the tissue distribution in these four individuals with that of four other patients with AL-amyloid derived from the L2-L16 gene. Although the interindividual variations were pronounced, liver and kidney involvement was much more evident in the latter four. CONCLUSIONS/SIGNIFICANCE: We conclude that although the use of a specific gene influences the tissue distribution of amyloid, each light chain exhibits one or more determinants of organ-specificity, which originate from somatic mutations and post-translational modifications. Eventual identification of such determinants could lead to improved treatment of patients with AL amyloidosis

Proteins That Promote Filopodia Stability, but Not Number, Lead to More Axonal-Dendritic Contacts

Dendritic filopodia are dynamic protrusions that are thought to play an active role in synaptogenesis and serve as precursors to spine synapses. However, this hypothesis is largely based on a temporal correlation between filopodia formation and synaptogenesis. We investigated the role of filopodia in synapse formation by contrasting the roles of molecules that affect filopodia elaboration and motility, versus those that impact synapse induction and maturation. We used a filopodia inducing motif that is found in GAP-43, as a molecular tool, and found this palmitoylated motif enhanced filopodia number and motility, but reduced the probability of forming a stable axon-dendrite contact. Conversely, expression of neuroligin-1 (NLG-1), a synapse inducing cell adhesion molecule, resulted in a decrease in filopodia motility, but an increase in the number of stable axonal contacts. Moreover, RNAi knockdown of NLG-1 reduced the number of presynaptic contacts formed. Postsynaptic scaffolding proteins such as Shank1b, a protein that induces the maturation of spine synapses, increased the rate at which filopodia transformed into spines by stabilization of the initial contact with axons. Taken together, these results suggest that increased filopodia stability and not density, may be the rate-limiting step for synapse formation

Structural peculiarities of a truncated VκIII immunoglobulin light chain in myeloma with light chain deposition disease

We report on the primary sequence of the monoclonal immunoglobulin light chain (LC) REV involved in myeloma-associated light chain deposition disease (LCDD). This sequence was deduced from that of the corresponding complementary (c)DNA in bone marrow plasma cells. Products of three independent amplifications by polymerase chain reaction (PCR) were sequenced and found to be identical. The κ mRNA encoding this N-glycosylated LC showed an overall normal structure consisting of a VκIII segment rearranged to JκII. Direct N-terminal amino acid sequencing of the circulating monoclonal IgA2,κ showed identity with the bone marrow-derived sequence. The κ-chain presented several unusual features affecting both the leader sequence and the variable (V) region. Four unique amino acid substitutions were found at positions -8, -3, -2 and -1 in the leader sequence and probably resulted in an unusual cleavage by signal peptidase, thus making the LC truncated by one residue and accounting for its unique hydrophobic N-terminus: Ile-Ile-Leu. Additional peculiarities were observed in the V region, including a Thr74 → Asn substitution creating a N-glycosylation site, and Thr53 → Ile, which was only reported once among human κIII chains, in another LCDD case, and may be of special significance at a position usually harbouring a polar amino acid