Characteristics and distribution of landslides in the populated hillslopes of Bujumbura, Burundi

Accurate and detailed multitemporal inventories of landslides and their process characterization are crucial for the evaluation of landslide hazards and the implementation of disaster risk reduction strategies in densely-populated mountainous regions. Such investigations are, however, rare in many regions of the tropical African highlands, where landslide research is often in its infancy and not adapted to the local needs. Here, we have produced a comprehensive multitemporal investigation of the landslide processes in the hillslopes of Bujumbura, situated in the landslide-prone East African Rift. We inventoried more than 1200 landslides by combining careful field investigation and visual analysis of satellite images, very-high-resolution topographic data, and historical aerial photographs. More than 20% of the hillslopes of the city are affected by landslides. Recent landslides (post-1950s) are mostly shallow, triggered by rainfall, and located on the steepest slopes. The presence of roads and river quarrying can also control their occurrence. Deep-seated landslides typically concentrate in landscapes that have been rejuvenated through knickpoint retreat. The difference in size distributions between old and recent deep-seated landslides suggests the long-term influence of potentially changing slope-failure drivers. Of the deep-seated landslides, 66% are currently active, those being mostly earthflows connected to the river system. Gully systems causing landslides are commonly associated with the urbanization of the hillslopes. Our results provide a much more accurate record of landslide processes and their impacts in the region than was previously available. These insights will be useful for land management and disaster risk reduction strategies

Added value of regional landslide susceptibility analysis: the western branch of the East African Rift

Landslides have a strong impact on environments and societies in many regions worldwide. Nonetheless, for most of these regions, landslide susceptibility has not been assessed in detail, so that susceptibility assessments necessarily rely on relatively coarse global/continental models. The regional accuracy and/or geomorphological plausibility of these global/continental models can be questioned due to several reasons: (1) they have not been calibrated and validated for the region under consideration (2) they rely on few and simple covariates, and (3) when they are datadriven, they are calibrated using a global rather than a local dataset. Therefore, we investigated whether there is an added value of regional models that justifies the construction efforts and costs and whether these efforts should be focused on compiling the regional landslide inventory or on the quality of spatial covariates such as slope steepness and lithology. More specifically, we performed a regional susceptibility assessment in a tropical data-scarce environment (the western branch of the East African Rift, with an approximate extent of 100,000 km2) and assessed its added value compared to recent continental and global assessments in terms of model performance. First, we constructed three data-driven regional models using a historical inventory ( > 6000 occurrences) compiled in Google Earth. Second, we quantified the cost of making such an inventory, by looking at how the model quality was impacted by (1) the inventory size, and (2) the use of more accurate regional peak ground acceleration and geolithology covariates. Third, we compared the predictive power and geomorphological plausibility of the regional and global/continental models. Despite the fact that global/continental models are useful in terms of identifying landslide-prone areas, we observed that they were considerably outperformed by the regional models in terms of predictive power ( AUC > 15%) and plausibility. We also showed that the differences in model performance are linked to differences in the size and quality of the landslide inventory, rather than the use of more accurate, regional covariates. Within our study area, high-quality models can be obtained with inventories of 500 landslides, i.e. 15% of the total inventory: a further increase in inventory size did not lead to better predictions. In conclusion, we demonstrated that regional susceptibility assessments do lead to a better understanding of spatial patterns in landslide susceptibility and are characterized by significant improvements in performance, compared to global and continental products. For our study area, we showed that the construction of representative local inventories plays a determinative role in these improvements

Characteristics and causes of natural and human-induced landslides in a tropical mountainous region: the rift flank west of Lake Kivu (Democratic Republic of the Congo)

Tropical mountainous regions are often identified as landslide hotspots with growing population pressure. Anthropogenic factors are assumed to play a role in the occurrence of landslides in these densely populated regions, yet the relative importance of these human-induced factors remains poorly documented. In this work, we aim to explore the impact of forest cover dynamics, roads and mining activities on the characteristics and causes of landslides in the rift flank west of Lake Kivu in the Democratic Republic of the Congo (DR Congo). To do so, we compile a comprehensive multi-temporal inventory of 2730 landslides. The landslides are of different types and are grouped into five categories that are adapted to study the impact of human activities on slope stability: old (pre-1950s) and recent (post-1950s) deep-seated landslides, shallow landslides, landslides associated with mining and landslides associated with road construction. We analyse the landslides according to this classification protocol via frequency area statistics, frequency ratio distribution and logistic regression susceptibility assessment. We find that natural factors contributing to the cause of recent and old deep-seated landslides were either different or changed over time. Under similar topographic conditions, shallow landslides are more frequent, but of a smaller size, in areas where deforestation has occurred since the 1950s. We attribute this size reduction to the decrease in regolith cohesion due to forest loss, which allows for a smaller minimum critical area for landsliding. In areas that were already deforested in the 1950s, shallow landslides are less frequent, larger and occur on less steep slopes. This suggests a combined role between regolith availability and soil management practices that influence erosion and water infiltration. Mining activities increase the odds of landsliding. Landslides associated with mining and roads are larger than shallow landslides but smaller than the recent deep-seated instabilities, and they are controlled by environmental factors that are not present under natural conditions. Our analysis demonstrates the role of human activities on the occurrence of landslides in the Lake Kivu region. Overall, it highlights the need to consider this context when studying hillslope instability characteristics and distribution patterns in regions under anthropogenic pressure. Our work also highlights the importance of using landslide classification criteria adapted to the context of the Anthropocene

Historical aerial Photographs and ArchiveS to assess Environmental Changes in Central Africa

Context: The conversion of natural lands into human-dominated landscapes is a major component of global environmental change. To quantify and understand the complexity of changes and subtle modifications in the environment, an accurate account of past landscape conditions has an important added value. However, sufficiently long, multidecadal records of landscape changes are almost inexistent for the least developed areas of the globe. Objectives: In PAStECA, we make use and valorise the Royal Museum for Central Africa (RMCA) s unique collection of historical aerial photographs (and other archives) to reveal key information on the transformation of the environment from the mid of the 20th century and its impacts on geo-hydrological hazards and/or risks such as landslides, gully erosion and volcanic eruptions in the western branch of the East African Rift (Burundi, DR Congo, Rwanda). Results: First, we develop custom-made scanning and georeferencing protocols and tools of the photographs and produce digital orthomosaics. Then, machine learning models are developed to extract land use and land cover information from these georeferenced digital products. These photographs dating back to the 1950 s are also used to quantify the impact of human activities on landslide rates. Covering over 60 years, we show that population dynamics, conflicts and deforestation affect geo-hydrological hazards and/or risks in both rural and urban contexts, with differences between the three countries. Conclusion: Through the production of new tools and models we evidence the need to preserve and valorise the aerial photographs and archives conserved at the RMCA to support present-day environmental change studies, especially in Central Africa

Transgene Silencing and Transgene-Derived siRNA Production in Tobacco Plants Homozygous for an Introduced AtMYB90 Construct

Transgenic tobacco (Nicotiana tabacum) lines were engineered to ectopically over-express AtMYB90 (PAP2), an R2–R3 Myb gene associated with regulation of anthocyanin production in Arabidopsis thaliana. Independently transformed transgenic lines, Myb27 and Myb237, accumulated large quantities of anthocyanin, generating a dark purple phenotype in nearly all tissues. After self-fertilization, some progeny of the Myb27 line displayed an unexpected pigmentation pattern, with most leaves displaying large sectors of dramatically reduced anthocyanin production. The green-sectored 27Hmo plants were all found to be homozygous for the transgene and, despite a doubled transgene dosage, to have reduced levels of AtMYB90 mRNA. The observed reduction in anthocyanin pigmentation and AtMYB90 mRNA was phenotypically identical to the patterns seen in leaves systemically silenced for the AtMYB90 transgene, and was associated with the presence of AtMYB90-derived siRNA homologous to both strands of a portion of the AtMYB90 transcribed region. Activation of transgene silencing in the Myb27 line was triggered when the 35S::AtMYB90 transgene dosage was doubled, in both Myb27 homozygotes, and in plants containing one copy of each of the independently segregating Myb27 and Myb237 transgene loci. Mapping of sequenced siRNA molecules to the Myb27 TDNA (including flanking tobacco sequences) indicated that the 3′ half of the AtMYB90 transcript is the primary target for siRNA associated silencing in both homozygous Myb27 plants and in systemically silenced tissues. The transgene within the Myb27 line was found to consist of a single, fully intact, copy of the AtMYB90 construct. Silencing appears to initiate in response to elevated levels of transgene mRNA (or an aberrant product thereof) present within a subset of leaf cells, followed by spread of the resulting small RNA to adjacent leaf tissues and subsequent amplification of siRNA production

Flemish network on rare connective tissue diseases (CTD): patient pathways in systemic sclerosis. First steps taken.

peer reviewedDespite the low prevalence of each rare disease, the total burden is high. Patients with rare diseases encounter numerous barriers, including delayed diagnosis and limited access to high-quality treatments. In order to tackle these challenges, the European Commission launched the European Reference Networks (ERNs), cross-border networks of healthcare providers and patients representatives. In parallel, the aims and structure of these ERNs were translated at the federal and regional levels, resulting in the creation of the Flemish Network of Rare Diseases. In line with the mission of the ERNs and to ensure equal access to care, we describe as first patient pathways for systemic sclerosis (SSc), as a pilot model for other rare connective and musculoskeletal diseases. Consensus was reached on following key messages: 1. Patients with SSc should have multidisciplinary clinical and investigational evaluations in a tertiary reference expert centre at baseline, and subsequently every three to 5 years. Intermediately, a yearly clinical evaluation should be provided in the reference centre, whilst SSc technical evaluations are permissionably executed in a centre that follows SSc-specific clinical practice guidelines. In between, monitoring can take place in secondary care units, under the condition that qualitative examinations and care including interactive multidisciplinary consultations can be provided. 2. Patients with early diffuse cutaneous SSc, (progressive) interstitial lung disease and/or pulmonary arterial hypertension should undergo regular evaluations in specialised tertiary care reference institutions. 3. Monitoring of patients with progressive interstitial lung disease and/or pulmonary (arterial) hypertension will be done in agreement with experts of ERN LUNG

A transgenic dTph1 insertional mutagenesis system for forward genetics in mycorrhizal phosphate transport of Petunia

The active endogenous dTph1 system of the Petunia hybrida mutator line W138 has been used in several forward-genetic mutant screens that were based on visible phenotypes such as flower morphology and color. In contrast, defective symbiotic phosphate (Pi) transport in mycorrhizal roots of Petunia is a hidden molecular phenotype as the symbiosis between plant roots and fungi takes place below ground, and, while fungal colonization can be visualized histochemically, Pi transport and the activity of Pi transporter proteins cannot be assessed visually. Here, we report on a molecular approach in which expression of a mycorrhiza-inducible bi-functional reporter transgene and insertional mutagenesis in Petunia are combined. Bi-directionalization of a mycorrhizal Pi transporter promoter controlling the expression of two reporter genes encoding firefly luciferase and GUS allows visualization of mycorrhiza-specific Pi transporter expression. A population of selectable transposon insertion mutants was established by crossing the transgenic reporter line with the mutator W138, from which the Pitransporter downregulated (ptd1) mutant was identified, which exhibits strongly reduced expression of mycorrhiza-inducible Pi transporters in mycorrhizal roots

Isolation and functional characterisation of banana phytoene synthase genes as potential cisgenes

Carotenoids occur in all photosynthetic organisms where they protect photosystems from auto-oxidation, participate in photosynthetic energy-transfer and are secondary metabolites. Of the more than 600 known plant carotenoids, few can be converted into vitamin A by humans and so these pro-vitamin A carotenoids (pVAC) are important in human nutrition. Phytoene synthase (PSY) is a key enzyme in the biosynthetic pathway of pVACs and plays a central role in regulating pVAC accumulation in the edible portion of crop plants. Bananas are a major commercial crop and serve as a staple crop for more than 30 million people. There is natural variation in fruit pVAC content across different banana cultivars, but this is not well understood. Therefore, we isolated PSY genes from banana cultivars with relatively high (cv. Asupina) and low (cv. Cavendish) pVAC content. We provide evidence that PSY in banana is encoded by two paralogs (PSY1 and PSY2), each with a similar gene structure to homologous genes in other monocots. Further, we demonstrate that PSY2 is more highly expressed in fruit pulp compared to leaf. Functional analysis of PSY1 and PSY2 in rice callus and E. coli demonstrate that both genes encode functional enzymes, and that Asupina PSYs have approximately twice the enzymatic activity of the corresponding Cavendish PSYs. These results suggest that differences in PSY enzyme activity contribute significantly to the differences in Asupina and Cavendish fruit pVAC content. Importantly, Asupina PSY genes could potentially be used to generate new cisgenic or intragenic banana cultivars with enhanced pVAC content