Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

Molecular typing ofStaphylococcus aureusbased on PCR-RFLP ofcoagene and RAPD analysisThe aim of this study was molecular identification ofS. aureusstrains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains thegapgene (930 bp) was amplified, which enabled their affiliation to theStaphylococcusgenus to be established. PCR-RFLP withAluI endonuclease of thegapgene as well asnuc(450 bp) andcoa(1130 bp) gene amplification allowed preciseS. aureusspecies identification. One hundred percent of the genetic relationship between strains was establishedviaRAPD-PCR and coa-typing.</jats:p

The presence of anti-Yersinia pseudotuberculosis immunoglobulins in equine serum

The research was conducted on clinically healthy mares (n=40) and foals (n=78) duringY. pseudotuberculosis associated enzootics. The animals were divided into groups: I to IV – mares, IA to IVA – their offsprings, IB to IVB – foals which mothers were not treated with any medicaments. The animals in group I, IA and IB were injected with PBS; in group II, IIA and IIB – with Y. pseudotuberculosis strain-based vaccine, in group III, IIIA and IIIB – with P. acnes strain-based immunostimulator; in group IV, IVA and IVB – with P. acnes strain-based immunostimulator and (5 days after the immunostimulator injection)Y. pseudotuberculosis strain-based vaccine. The presence of antibodies was determined by means of ELISA. The study revealed anti-Yersinia pseudotuberculosis IgG only in 19 mares before, and in 25 mares and 26 foals 3 weeks after vaccination. The mean extinction 3 weeks after vaccination amounted to: II-0.489, IV-2.578, IIA-0.572, IVA-0.974, IIB-0.312, IVB-0.418. The cut-off extinction value was 0.154. The presence of anti-Yersinia pseudotuberculosis IgG before vaccination in the sera of clinically healthy mares may suggest that Y. pseudotuberculosis infection occurs definitely more often than is expected. Vaccination preceded by immunostimulation appeared to be the most efficient method of treatment against yersiniosis

Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing

Estimation of the efficiency of active immunization of farrow sows with vaccine in the prophylaxy of colibacteriosis of suckling piglets taking into consideration different drug administration ways

Целью настоящего труда была оценка эффективности 5 видов вакйин изготовляемых на базе местного энтеротоксичного серотина Е. coli (Мун 987) в борьбе с колибактериозом сосущим поросят. Вакцинации подвергали 3 группы супоросных свиноматок (по 10 голов), применяя инактивированные вакцины перорально и подкожно, а жизнеспособную вакцину перорально. Потомство указанных свиноматок заражали непосредственно после рождения штаммом Мун 1413, имеющим только один общий компонент со штаммом Мун 987 - фимбриальный антиген. Установлено, что иммунизация супоросных свиноматок вакцинами изготовленными на базе местных фимбриальных энтеротоксичных штаммов Е. coli хранит их потомсто против болезни. Она приводит также к лучшему росту поросят на 1-ой неделе жизни и позволяет сократить период предродовой акклиматизации супоросных свиноматок. Установлено, что наиболее эффективно действует вакцина содержащая живые бактерии подаваемая перорально и инактивированная вакцина подаваемая подкожно.The aim of the respective investigations was to assess the efficiency of 3 vaccine kinds produced on the basis of a local enterotoxic E. coli serotype (Moon 987) in the prophylaxy of colibacteriosis of suckling piglets. Three groups of farrow sows (by 10 heads) at administration of the inactivated vaccines per os and subcutaneously and the vital vaccine per os. The offsping of these sows was infected close after birth with the enterotoxin Moon 987 strain - fimbrial antigen. It has been found that the immunization of farrow sows with vaccines prepared on the basis of local, fimbrial enterotxic E. coli strains protects their offspring against the disease. It results also in a better growth of piglets in the 1st week of life and allows to shorten the prepartum acclimatization period of farrow sows. It has been found that the most efficient is the vaccine containing living bacteria administered per os and the inactivated vaccine administered subcutaneously