Observation of Spin-Dependent Charge Symmetry Breaking in ΛN\Lambda N Interaction: Gamma-Ray Spectroscopy of Λ4^4_{\Lambda }He

The energy spacing between the ground-state spin doublet of $^4_\Lambda$He(1$^+$,0$^+$) was determined to be $1406 \pm 2 \pm 2$ keV, by measuring $\gamma$ rays for the $1^+ \to 0^+$ transition with a high efficiency germanium detector array in coincidence with the $^4$He$(K^-,\pi^-)$ $^4_\Lambda$He reaction at J-PARC. In comparison to the corresponding energy spacing in the mirror hypernucleus $^4_\Lambda$H, the present result clearly indicates the existence of charge symmetry breaking (CSB) in $\Lambda N$ interaction. It is also found that the CSB effect is large in the $0^+$ ground state but is by one order of magnitude smaller in the $1^+$ excited state, demonstrating that the $\Lambda N$ CSB interaction has spin dependence

Plasmid pP62BP1 isolated from an Arctic Psychrobacter sp. strain carries two highly homologous type II restriction-modification systems and a putative organic sulfate metabolism operon

The complete nucleotide sequence of plasmid pP62BP1 (34,467 bp), isolated from Arctic Psychrobacter sp. DAB_AL62B, was determined and annotated. The conserved plasmid backbone is composed of several genetic modules, including a replication system (REP) with similarities to the REP region of the iteron-containing plasmid pPS10 of Pseudomonas syringae. The additional genetic load of pP62BP1 includes two highly related type II restriction-modification systems and a set of genes (slfRCHSL) encoding enzymes engaged in the metabolism of organic sulfates, plus a putative transcriptional regulator (SlfR) of the AraC family. The pP62BP1 slflocus has a compact and unique structure. It is predicted that the enzymes SlfC, SlfH, SlfS and SlfL carry out a chain of reactions leading to the transformation of alkyl sulfates into acyl-CoA, with dodecyl sulfate (SDS) as a possible starting substrate. Comparative analysis of the nucleotide sequences of pP62BP1 and other Psychrobacter spp. plasmids revealed their structural diversity. However, the presence of a few highly conserved DNA segments in pP62BP1, plasmid 1 of P. cryohalolentis K5 and pRWF-101 of Psychrobacter sp. PRwf-1 is indicative of recombinational shuffling of genetic information, and is evidence of lateral gene transfer in the Arctic environment

Gamma decay of pygmy states in 90,94Zr from inelastic scattering of light ions

We performed experiments to study the low-energy part of the E1 response (Pygmy Dipole Resonance) in 90,94Zr nuclei, by measuring the (p,p'γ) and (α,α'γ) inelastic scattering reactions at energies Ebeam,p = 80 MeV and Ebeam,α = 130 MeV respectively. The inelastically scattered particles were measured by employing the high-resolution spectrometer Grand Raiden. The gamma-rays emitted following the de-excitation of the Zr target nuclei were detected using both the clover type HPGe detectors of the CAGRA array and the large volume LaBr3:Ce scintillation detectors from the HECTOR+ array. Some preliminary results are presented here

Search for in-band transitions in the candidate superdeformed band in Si 28

Background: Superdeformed (SD) bands are suggested by theory around Ca40 and in lighter alpha-conjugate nuclei such as Mg24, Si28, and S32. Such predictions originate from a number of theoretical models including mean-field models and antisymmetrized molecular dynamics (AMD) calculations. While SD bands have been identified in Ca40 and its near neighbors, evidence of their existence in the lighter, midshell nuclei is circumstantial at best. The key evidence of superdeformation would be the observation of transitions with high B(E2) transition strengths connecting states in a rotational sequence. This is challenging information to obtain since the bands lie at a high excitation energy and competition from out-of-band decay is dominant. Purpose: The purpose of the present study is to establish a new methodology to circumvent the difficulties in identifying and quantifying in-band transitions through directly populating candidate states in the SD band in Si28 through inelastic alpha scattering, selecting such states with a spectrometer, and measuring their gamma-ray decay with a large array of high-purity germanium detectors, allowing direct access to electromagnetic transition strengths. Methods: Excited states in Si28 were populated in the Si28(α,α′) reaction using a 130-MeV He4 beam from the K140 AVF cyclotron at the Research Center for Nuclear Physics. Outgoing alpha particles were analyzed using the Grand Raiden spectrometer positioned at an angle of 9.1° to favor the population of states with J≈4. Coincident gamma rays were detected with the CAGRA array of 12 HPGe clover detectors augmented by a set of four large LaBr3 detectors. Results: Data analysis showed that it was possible to identify additional low-energy transitions in competition with high-energy decays from excited states in Si28 in the vicinity of 10 MeV. However, while the candidate 4+ SD state at 10.944 MeV was populated, a 1148-keV transition to the candidate 2+ SD state at 9.796 MeV was not observed, and only an upper limit for its transition strength of B(E2)<43 W.u. could be established. This contradicts AMD predictions of ≈200 W.u. for such a transition. Conclusion: The present study strongly rejects the hypothesis that the candidate set of states identified in Si28 represents an SD band, which demonstrates the potential of the methodology devised here

Low-temperature study of 35 photomultiplier tubes for the ZEPLIN III experiment

A set of 35 photomultiplier tubes (ETL D730/9829Q), intended for use in the ZEPLIN III Dark Matter detector, was tested from room temperature down to -100°C, with the aim of confirming their suitability for detecting xenon scintillation light at 175 nm while immersed in the cryogenic liquid. A general improvement of both gain and quantum efficiency at the xenon scintillation wavelength was observed with cooling, the best combined effect being 40%, while little change was noted in the timing properties and dark current. Saturation of response due to accumulation of charge in the resistive bialkali photocathodes was seen at an average photocurrent of 108 photoelectrons/s for the device with best quantum efficiency, whereas an order of magnitude higher current was required to saturate the least sensitive one. Variations in photocathode thickness from tube to tube could account for this behaviour, as well as the fact that the quantum efficiency improves the most for devices with poorest efficiency at room temperature.http://www.sciencedirect.com/science/article/B6TJM-4B54Y87-7/1/1f3b109a1dce0f3de7e3225f216189e

Isolation and characterization of mutants of a marine Vibrio strain that are defective in production of extracellular proteins.

A marine Vibrio strain, Vibrio sp. strain 60, produces several extracellular proteins, including protease, amylase, DNase, and hemagglutinin. Mutants of Vibrio sp. strain 60 (epr mutants) pleiotropically defective in production of these extracellular proteins were isolated. They fell into two classes, A and B. In class A, no protease activity was detected in the cells either, whereas in class B, considerable protease activity was detected in the cells. Gel electrophoretic analysis revealed that the protease detected in class B mutant cells was similar to the protease excreted by the parent strain. In addition, the protease in class B mutant cells was found to be localized in the periplasmic space. These results suggest that the passage of the protease through the outer membrane is blocked in class B mutants. Comparison of membrane protein profiles by polyacrylamide gel electrophoresis revealed that all the epr mutants contained an increased amount of a 94,000-Mr protein that may be an outer membrane protein. Four epr mutations were mapped in two different regions of the Vibrio chromosome by transduction; two class A mutations and one class B mutation were located close to each other, whereas another class B mutation was located in a different region of the chromosome

Genetic analysis of Rhizobium meliloti bacA-phoA fusion results in identification of degP: two loci required for symbiosis are closely linked to degP.

The function of the Rhizobium meliloti bacA gene, which is a homolog of the Escherichia coli sbmA gene, is required for an intermediate step in nodule development. A strain carrying the bacA386::TnphoA fusion was mutagenized with N-methyl-N'-nitro-N-nitrosoguanidine, and three mutants that had higher levels of alkaline phosphatase activity were identified. The mutations in these strains were recessive and mapped to the same genetic locus. The gene affected by these mutations was identified and sequenced and was found to be a homolog of the E. coli degP gene, which encodes a periplasmic endopeptidase. Although degP function is important for the virulence of certain intracellular pathogens of mammals, it is not required for the R. meliloti-alfalfa symbiosis. The genetic analyses involving degP were complicated by the presence of a locus immediately upstream of depP that was lethal when present in multiple copies in a DegP- background. R. meliloti derivatives carrying insertion mutations in this locus displayed an N,N,N',N'-tetramethyl-p-phenylenediamine oxidase-negative phenotype, elicited the formation of white cylindrical nodules that did not fix nitrogen, and grew slowly in rich medium, suggesting that the locus was a cyc gene encoding a protein involved in the biosynthesis of a component or components of a respiratory chain. The previously identified fix-382::TnphoA, which similarly causes the formation of white cylindrical nodules that do not fix nitrogen, was shown to affect a gene that is separate from this cyc gene but extremely closely linked to it