Metabonomics and Intensive Care

This article is one of ten reviews selected from the Annual Update in Intensive Care and Emergency medicine 2016. Other selected articles can be found online at http://www.biomedcentral.com/collections/annualupdate2016. Further information about the Annual Update in Intensive Care and Emergency Medicine is available from http://www.springer.com/series/8901

Pulmonary Lymphangiomyomatosis in Bourneville’s Tuberous Sclerosis: Case Report

Lymphangiomyomatosisis a rare disease characterized by a proliferation of abnormal smooth muscle cells responsible for infiltration with the destruction of tissue architecture and genesis of cystic lung and lymphatic lesions. In addition to lung damage, Bourneville’s tuberous sclerosis (BTS) also affects the skin, brain, retina, kidneys, and, less frequently, the heart and bone. We report the case of a young patient with bilateral pneumothorax revealing pulmonary lymphangiomyomatosis in the context of Bourneville’stuberoussclerosis BTS

Efficacy of prenatal ultrasonography in diagnosing urogenital developmental anomalies in newborns.

BACKGROUND: Showing a prevalence rate of 0.5-0.8%, urogenital malformations discovered in newborns is regarded relatively common. The aim of this study is to examine the efficacy of ultrasound diagnostics in detecting developmental disorders in the urogenital system. METHODS: We have processed the prenatal sonographic and postnatal clinical details of 175 urogenital abnormalities in 140 newborns delivered with urogenital malformation according to EUROCAT recommendations over a 5-year period between 2006 and 2010. The patients were divided into three groups; Group 1: prenatal sonography and postnatal examinations yielded fully identical results. Group 2: postnatally detected urogenital changes were partially discovered in prenatal investigations. Group 3: prenatal sonography failed to detect the urogenital malformation identified in postnatal examinations. Urogenital changes representing part of certain multiple disorders associated with chromosomal aberration were investigated separately. RESULTS: Prenatal sonographic diagnosis and postnatal results completely coincided in 45%, i.e. 63/140 of cases in newborns delivered with urogenital developmental disorders. In 34/140 cases (24%), discovery was partial, while in 43/140 patients (31%), no urogenital malformation was detected prenatally. No associated malformations were observed in 108 cases, in 57 of which (53%), the results of prenatal ultrasonography and postnatal examinations showed complete coincidence. Prenatally, urogenital changes were found in 11 patients (10%), whereas no urogenital disorders were diagnosed in 40 cases (37%) by investigations prior to birth. Urogenital disorders were found to represent part of multiple malformations in a total of 28 cases as follows: prenatal diagnosis of urogenital malformation and the findings of postnatal examinations completely coincided in three patients (11%), partial coincidence was found in 22 newborns (79%) and in another three patients (11%), the disorder was not detected prenatally. In four newborns, chromosomal aberration was associated with the urogenital disorder; 45,X karyotype was detected in two patients, trisomy 9 and trisomy 18 were found in one case each. CONCLUSION: In approximately half of the cases, postnatally diagnosed abnormalities coincided with the prenatally discovered fetal urogenital developmental disorders. The results have confirmed that ultrasonography plays an important role in diagnosing urogenital malformations but it fails to detect all of the urogenital developmental abnormalities

GENETIC IMPROVEMENT OF KANINJOT VARIETY FROM CLONAL SELECTION

Abstract: The "Kaninjot" cultivar, autochthonous and widespread is remarkable for high percentage and good quality of the oil. It has dual use. It has periodical production, but the variability that this genotype possesses has been exploited through clonal selection to find individuals with high production constant, oil percentage and bigger average fruit weight. The selection underwent two phases: In the first phase: over a population of 2583 trees, in a five year period, where each year the trees that were qualified had inflorescence and fructified, while the others were considered out of use. In the second phase for 17 clone candidates derived from selection of the first phase production evaluation was done for kg/trees/ year, (i) oil percentage % fresh matter done through Soxhlet, (iii) average fruit weight (g), (iv) production periodicity estimated through Pearce and Dobersek-Urbank. Each clone displayed differently its genetic potentials, with differences for production constant. Constant production had 6 individuals (0.00 -0.30). The variability for fruit weight was from 0.26 g up to 1.24 g more than the standard (12 individual). For the oil percentage 6 individuals with 0.1 up to 2.1% more than the average of the population were selected. In the final evaluation there resulted to be: (i) 12 clones with superior PC. (ii) 6 clones possess superior for constant production and oil percentage (PC+OP). (iii) 6 clones possess the three selection features; constant production, oil percentage and fruit weight (PC+Fw+Op). The selected individuals have differences as far as the pomological and technological characteristics are concerned

Chemical variability and acaricidal activity of Rosmarinus officinalis L. essential oils

Rosemary leaves are characterized by two main periods of essential oils production and contain high levels of oils (2.11 to 3.13%). Their oils, extracted by hydrodistillation and analyzed by GC-FID and GC-MS, are dominated by oxygenated and hydrocarbons monoterpens and contain high amounts of 1,8-cineole. The chemical composition didn’t vary drastically during the phenologic stages, but a noticeable variability is however found within the ten studied populations. PCA and regression analysis revealed that among factors regulating the biosynthesis of oils during the different growth stages of the plant, the mean monthly temperature and monthly precipitation would be determinant. In hives bioassays, the use of absorbent paper pad impregnated by pure rosemary oil for six days showed certain effectiveness against Varroa mites. So, an application rate of 5ml of rosemary oil caused the highest mites mortalities, but the concentration of 3ml that producing an equivalent toxicity to flumetrin, is recommended. To overcome the phenomenon of resistance to chemicals widely used in apiaries infested by Varroa destructor, the use of pure rosemary oils as biopesticide for controlling acarian pests would be an appreciated tool

Establishment an empirical relationship equation of magnitude conversion specific for Malaysia

This paper presents the results of the establishment an empirical relationship equation of magnitude conversion specific for Malaysia. The data for this study is taken from the earthquake catalogue and that is from Incorporated Research Institutions for Seismology (IRIS), National Earthquake Information Centre (NEIC)-U.S. Geological Survey (USGS), European-Mediterranean Seismological Centre (EMSC) and Malaysian Meteorological Service (MMS) within the latitude of 9 North and-3 South, and longitude of 122 East and 95 West from 1 January 1900 until 31 December 2006. There are two empirical relationship equation will be developed for Malaysia through regression method and there are empirical relationship equation of surface wave magnitude, M s constructed with records that have both surface wave magnitude, M s and body wave magnitude, m b and empirical relationship equation of moment magnitude, M w constructed with records that have both moment magnitude, M w and surface wave magnitude, M

La inoculación con bacterias del ácido acético mejora la calidad de las aceitunas de mesa verdes naturales

This study aims to develop a method for the preparation of natural table olives using locally selected microorganisms and without resorting to the usual techniques which employ lye treatment and acids. The effects of parameters, such as lye treatment, inoculation with yeasts, substitution of organic acids with vinegar and/or acetic acid bacteria, and finally alternating aeration have been assessed. Four different combinations were applied to the “Picholine marocaine” olive variety using indigenous strains, namely Lactobacillus plantarum S1, Saccharomyces cerevisiae LD01 and Acetobacter pasteurianus KU710511 (CV01) isolated respectively from olive brine, Bouslikhen dates and Cactus. Two control tests, referring to traditional and industrial processes, were used as references. Microbial and physicochemical tests showed that the L3V combination (inoculated with A. pasteurianus KU710511 and L. plantarum S1 under the optimal growth conditions of the Acetic Acid Bacteria (AAB) strain with 6% NaCl) was found to be favorable for the growth of the Lactic Acid Bacteria (LAB) strain which plays the key role in olive fermentation. This result was confirmed by sensory evaluation, placing L3V at the top of the evaluated samples, surpassing the industrial one where a chemical debittering treatment with lye was used. In addition, alternating aeration served to increase the microbial biomass of both AAB and LAB strains along with Saccharomyces cerevisiae LD01 strain, but also to use lower concentration of NaCl and to reduce the deterioration of olives compared to the anaerobic fermentation process. Finally, a mixed starter containing the three strains was prepared in a 10-L Lab-fermenter from the L3V sample in order to improve it in subsequent studies. The prepared starter mixture could be suitable for use as a parental strain to prepare table olives for artisan and industrial application in Morocco.Este estudio tiene como objetivo desarrollar un método para la preparación de aceitunas de mesa naturales utilizando microorganismos seleccionados localmente y sin recurrir a las técnicas habituales que utilizan el tratamiento con lejía y ácidos. Se han evaluado los efectos de parámetros como el tratamiento con lejía, la inoculación con levaduras, la sustitución de ácidos orgánicos por bacterias de vinagre y/o ácido acético, y finalmente la aireación alterna. Se ensayaron cuatro combinaciones diferentes en la variedad de aceituna «Picholine marocaine» utilizando cepas autóctonas, como Lactobacillus plantarum S1, Saccharomyces cerevisiae LD01 y Acetobacter pasteurianus KU710511 (CV01) aisladas respectivamente de salmuera de aceitunas, Bouslikhen y Cactus. Se utilizaron como referencia dos pruebas de control, referidas a procesos tradicionales e industriales. Las pruebas microbianas y fisicoquímicas mostraron que la combinación L3V (inoculada con A. pasteurianus KU710511 y L. plantarum S1 en las condiciones óptimas de crecimiento de la bacteria del ácido acético (AAB) con NaCl al 6%) resultó ser favorable para el crecimiento del Láctico. Las cepas de bacterias ácidas (LAB) juegan un papel clave en la fermentación de las aceitunas. Este resultado fue confirmado por la evaluación sensorial colocando L3V en la parte superior de las muestras evaluadas, superando a la industrial, donde se usó un tratamiento de eliminación química con lejía. Además, la aireación alterna permitió aumentar la biomasa microbiana de las cepas AAB y LAB junto con la cepa Saccharomyces cerevisiae LD01, también usar una concentración más baja de NaCl y reducir el deterioro de las aceitunas, en comparación con la operación de fermentación anaerobia. Finalmente, se preparó un iniciador mixto que contenía las tres cepas en un fermentador de laboratorio de 10-L a partir de la muestra L3V con el objeto de mejorarlo en los estudios posteriores. El iniciador de mezcla preparado podría ser adecuado para usarse como una cepa parental para preparar aceitunas de mesa para aplicaciones artesanales e industriales en Marruecos

The susceptibility of Escherichia coli strains to essential oils of Rormarinus officinalis and Eucalyptus globulus

Essential oils are frequently used for flavour and fragrance in the perfume, pharmaceutical, cosmetic and food industries. They are also suitable raw material for production of new synthetic agents. The antibacterial activity of the essential oils obtained by steam distillation of Rosmarinus officinalis L and Eucalyptus globules collected in south Morocco was studied by micro-atmospheric technique against Escherichia coli CIP54127 and E. coli isolated from urine and resistant to several antibiotics. Both essential oils were effective against the two strains bacteria in particularly against E. coli CIP54127. MIQ of both oils ranged from 60 to 70 ìl for both bacteria. In conclusion, results show that essential oils are effective and could be considered as a natural antimicrobial source that can be used on production of natural antimicrobial agents

Unveiling the Marvels of 3D Echo: Illuminating prosthetic mitral valve dehiscence through 3D transillumination

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