Comparison of Integrated Digital Radiometer with Concurrent Water Vapor Radiometer using the Alphasat Receivers in Milan, Italy

In June 2014, NASA Glenn Research Center (GRC) and the Politecnico di Milano (POLIMI) jointly deployed a pair of coherent 20 GHz and 40 GHz beacon receivers to the POLIMI campus in Milan, Italy to characterize the atmospheric channel at Ka- and Q-band within the framework of the Alphasat experiment. The Milan receivers observe the continuous-wave beacons broadcast over Europe by the Aldo Paraboni Technology Demonstration Payload (TDP #5), and, in September 2017, both channels were upgraded to incorporate a novel digital radiometer (DR) measurement which NASA has recently employed in other propagation measurement campaigns. In November 2016, a co-located water vapor radiometer (WVR) was also installed at POLIMI, and the concurrent data from both the WVR and DR thusly enables validation of this new DR technique against the established WVR. Herein, we preliminarily investigate the calibration of the DR measurements using the WVR data and also assess a calibration method that may be implemented where WVR data is not readily available

Sphingolipid metabolic flow controls phosphoinositide turnover at the trans Golgi network

Sphingolipids are membrane lipids, which are globally required for eukaryotic life. Sphingolipid composition varies among endomembranes with pre- and post-Golgi compartments being poor and rich in sphingolipids, respectively. Thanks to this different sphingolipid content, pre- and post-Golgi membranes serve different cellular functions. Nevertheless, how subcellular sphingolipid levels are maintained in spite of trafficking and metabolic fluxes is only partially understood. Here we describe a homeostatic control circuit that controls sphingolipid levels at the trans Golgi network. Specifically, we show that sphingomyelin production at the trans Golgi network triggers a signalling reaction leading to PtdIns(4)P dephosphorylation. Since PtdIns(4)P is required for cholesterol, and sphingolipid transport to the trans Golgi network, PtdIns(4)P consumption leads to the interruption of this transport in response to excessive sphingomyelin production. Based on this evidence we envisage a model where this homeostatic circuit maintains the lipid composition of trans Golgi network and thus of post-Golgi compartments constant, against instant fluctuations in the sphingolipid biosynthetic flow.Peer ReviewedPostprint (author's final draft

Genomic and transcriptomic comparison between Staphylococcus aureus strains associated with high and low within herd prevalence of intra-mammary infection

Background: Staphylococcus aureus (Staph. aureus) is one of the major pathogens causing mastitis in dairy ruminants worldwide. The chronic nature of Staph. aureus infection enhances the contagiousness risk and diffusion in herds. In order to identify the factors involved in intra-mammary infection (IMI) and diffusion in dairy cows, we investigated the molecular characteristics of two groups of Staph. aureus strains belonging to ST8 and ST398, differing in clinical properties, through comparison of whole genome and whole transcriptome sequencing. Results: The two groups of strains, one originated from high IMI prevalence herds and the other from low IMI prevalence herds, present a peculiar set of genes and polymorphisms related to phenotypic features, such as bacterial invasion of mammary epithelial cells and host adaptation. Transcriptomic analysis supports the high propensity of ST8 strain to chronicity of infection and to a higher potential cytotoxicity. Conclusions: Our data are consistent with the invasiveness and host adaptation feature for the strains GTB/ST8 associated to high within-herd prevalence of mastitis. Variation in genes coding for surface exposed proteins and those associated to virulence and defence could constitute good targets for further research