Genetic variation and quantitative trait loci associated with developmental stability and the environmental correlation between traits in maize

There is limited experimental information about the genetic basis of micro-environmental variance (V-E) (developmental stability) and environmental correlations. This study, by using a population of maize recombinant inbred lines (RIL) and simple sequence repeat (SSR) polymorphic markers, aims at the following: firstly, to quantify the genetic component of the V-E or developmental stability for four traits in maize and the environmental correlation between these traits, and secondly, to identify quantitative trait loci (QTLs) that influence these quantities. We found that, when estimating variances and correlations and testing their homogeneity, estimates and tests are needed that are not highly dependent on normality assumptions. There was significant variation among the RILs in V-E and in the environmental correlation for some of the traits, implying genetic heterogeneity in the V-E and environmental correlations. The genetic coefficient of variation of the environmental variance (GCVV(E)) was estimated to be 20%, which is lower than estimates obtained for other species. A few genomic regions involved in the stability of one trait or two traits were detected, and these did not have an important influence on the mean of the trait. One region that could be associated with the environmental correlations between traits was also detected.</p

Defensive changes in maize leaves induced by feeding of Mediterranean corn borer larvae

Bioassays raw data. (XLSX 33 kb

Contribution of autochthonous maize populations for adaptation to European conditions

Early vigor, earliness and cold tolerance are the main potential contributions of European maize (Zea mays L.) for breeding programs for adaptation to areas with short growing seasons and cold springs. The objective of this research was to determine the potential contributions of populations from different European regions to breeding for adaptation. Six Spanish and six French maize populations differing on variability for earliness, vigor and cold tolerance were crossed in a complete diallel without reciprocals. The populations and their crosses were evaluated in the field and in a cold chamber. Minimum temperatures were the main environmental trait affecting genotype × environment interaction, probably due to the cold sensitivity of the genotypes with the best performance in the field. The best population cross, based on specific heterosis for adaptation-related traits in the field, was Viana × Rastrojero, but this cross was cold sensitive. Tuy × Lazcano should be the best choice for a breeding program for adaptation, based on performance in the field and cold tolerance. As conclusions, there was variability for earliness, vigor and cold tolerance among the populations and crosses involved in this study, being tolerant to cold conditions the populations with medium growing cycle originated in areas with short growing seasons. The highest yielding crosses were cold sensitive.Research supported by the Ministry of Science and Technology (Ref. HF1999-0138), the Ministère de l’Education Nationale et de la Recherche, the Committee for Science and Technology of Spain (Project AGL2004-06776), the Autonomous government of Galicia (PGIDIT04RAG403006PR), the Excma. Diputación Provincial de Pontevedra, and the European Union (RESGEN 88 CT96).Peer reviewe

Role of Dehydrodiferulates in Maize Resistance to Pests and Diseases

Phenolic esters have attracted considerable interest due to the potential they offer for peroxidase catalysed cross-linking of cell wall polysaccharides. Particularly, feruloyl residues undergo radical coupling reactions that result in cross-linking (intra-/intermolecular) between polysaccharides, between polysaccharides and lignin and, between polysaccharides and proteins. This review addresses for the first time different studies in which it is established that cross-linking by dehydrodiferulates contributes to maize’s defences to pests and diseases. Dehydrodiferulate cross-links are involved in maize defence mechanisms against insects such as the European, Mediterranean, and tropical corn borers and, storage pest as the maize weevil. In addition, cross-links are also discussed to be involved in genetic resistance of maize to fungus diseases as Gibberella ear and stalk rot. Resistance against insects and fungus attending dehydrodiferulates could go hand in hand. Quantitative trait loci mapping for these cell wall components could be a useful tool for enhancing resistance to pest and diseases in future breeding programs

Can a Growth Mindset Intervention Increase Individuals’ Intentions to Engage in Intergroup Dialogue?

Dialogue initiatives between different status groups foster meaningful conversations about inequality. Low and high status groups, however, frequently avoid discussions on difficult topics and do not participate in these initiatives. To address this, the current research tested an intervention model that aims to increase dialogue intentions using the growth mindset framework. The model proposes that inducing a malleable mindset toward one’s dialogue skills would boost group members’ intentions to engage with the outgroup. The intervention was hypothesized to be especially effective for high status groups, who have higher anxiety relative to lower status groups when anticipating intergroup dialogue. The intervention was predicted to work through two mediating processes: the reduction of anxiety and the increase in self-efficacy. The model was tested by conducting three studies with White Americans (high status; Studies 1-3) and two lower status groups: Black Americans (Studies 1 and 2) and Latinx Americans (Study 3). Across all studies, participants read a (fictional) scientific article about individual’s ability to develop effective dialogue skills, and then self-reported their intentions to participate in various dialogue-related activities. Studies 2 and 3 included measures of anxiety and self-efficacy. Analyses of variance revealed mixed support for the model. In Study 1, participants who received the growth mindset intervention had slightly higher intentions to engage in dialogue compared to those in the control condition. In Study 2, the intervention engendered lower intergroup anxiety for White Americans relative to Black Americans, though this reduction in anxiety did not lead to intentions to join a communication skills workshop or a dialogue initiative. Participants in the growth mindset condition in Study 3 had significantly less intentions to participate in a real-time intergroup discussion or to sign up for a pilot dialogue program on race, with this effect being more pronounced for Latinx compared to White Americans. Interestingly, self-efficacy across status groups and conditions were above average at baseline and remained unchanged. The discussion summarizes the findings in the context of the intervention model and proposes implications for previous research on dialogue between status groups. Finally, the significance of the results with regard to policy and practical applications are discussed

Managing maize under pest species competition: is Bt (Bacillus thuringiensis) maize the solution?

Transgenic crops that contain Cry genes from Bacillus thuringiensis (Bt) have been adopted by farmers over the last 17 years. Unlike traditional broad spectrum chemical insecticides, Bt's toxicity spectrum is relatively narrow and selective, which may indirectly benefit secondary insects that may become important pests. The economic damage caused by the rise of secondary pests could offset some or all of the benefits associated with the use of Bt varieties. We develop a bioeconomic model to analyze the interactions between primary and secondary insect populations and the impact of different management options on insecticide use and economic impact over time. Results indicate that some of the benefits associated with the adoption of genetically engineered insect resistant crops may be eroded when taking into account ecological dynamics. It is suggested that secondary pests could easily become key insect pests requiring additional measures - such as insecticide applications or stacked traits – to keep their populations under the economic threshold

QTL mapping for Mediterranean corn borer resistance in European flint germplasm using recombinant inbred lines

<p>Abstract</p> <p>Background</p> <p><it>Ostrinia nubilalis </it>(ECB) and <it>Sesamia nonagrioides </it>(MCB) are two maize stem borers which cause important losses in temperate maize production, but QTL analyses for corn borer resistance were mostly restricted to ECB resistance and maize materials genetically related (mapping populations derived from B73). Therefore, the objective of this work was to identify and characterize QTLs for MCB resistance and agronomic traits in a RILs population derived from European flint inbreds.</p> <p>Results</p> <p>Three QTLs were detected for stalk tunnel length at bins 1.02, 3.05 and 8.05 which explained 7.5% of the RILs genotypic variance. The QTL at bin 3.05 was co-located to a QTL related to plant height and grain humidity and the QTL at bin 8.05 was located near a QTL related to yield.</p> <p>Conclusions</p> <p>Our results, when compared with results from other authors, suggest the presence of genes involved in cell wall biosynthesis or fortification with effects on resistance to different corn borer species and digestibility for dairy cattle. Particularly, we proposed five candidate genes related to cell wall characteristics which could explain the QTL for stalk tunnelling in the region 3.05. However, the small proportion of genotypic variance explained by the QTLs suggest that there are also many other genes of small effect regulating MCB resistance and we conclude that MAS seems not promising for this trait. Two QTLs detected for stalk tunnelling overlap with QTLs for agronomic traits, indicating the presence of pleitropism or linkage between genes affecting resistance and agronomic traits.</p

Hydrologic and erosion responses to compaction and added surface cover in post-fire logged areas: isolating splash, interrill and rill erosion

Soil compaction during post-fire logging can increase runoff and erosion. Increasing surface cover is an effective way to reduce erosion, but this has not been tested on soils impacted by both fire and compaction. We measured the effects of compaction (bulk density of 0.9 or 1.1 g cm−3) and surface cover (0% or 60%) using bark mulch or logging slash (compacted only) on runoff, leaching and erosion. Four 0.5 m2 plots per treatment were filled with burned soil and inclined to 18°. Two 30-min simulated rainfall events with 72 mm h−1 intensity and one concentrated flow event were applied to each plot. The runoff from the dry and wet runs totaled 32 mm in the uncompacted bare plots and the value was not significantly different for the compacted bare plots, any of the mulch plots, or the compacted slash plots. Leaching was higher for the uncompacted bare plots (7 mm) than for the compacted bare plots (1 mm), and mulch or slash did not affect leaching. The uncompacted bare plots produced 38 g of rainsplash and 497 g m−2 of sheetwash, and these were about half the values for the compacted bare plots. Mulching strongly reduced the rainsplash and sheetwash for both the uncompacted (10 g and 184 g m−2, respectively) and compacted conditions (19 g and 431 g m−2, respectively), although the difference in sheetwash was only significant for the uncompacted plots. Slash also reduced rainsplash (19 g) relative to the compacted bare plots (74 g) but did not affect sheetwash. Concentrated flow produced 858 g m−2 of rilling from the uncompacted bare plots, which was greater than the compacted plots (237 g m−2). Mulching reduced rilling by 95% in the uncompacted plots and 78% in the compacted plots, but slash did not reduce rilling. The reductions in rainsplash erosion caused by the mulch and slash were attributed to the increase in surface cover, which reduced the energy imparted to the soil by the rainfall. The mulch, with its high degree of contact with the soil, also reduced particle detachment and transport by overland flow. In contrast, the slash did not have complete contact with the soil surface and did not affect sheetwash or rilling. Compaction and the type of material used to increase surface cover are important factors to consider when assessing potential impacts of logging on runoff and sediment delivery from burned soils.publishe

Experimental Studies on Bond Performance of BFRP bars reinforced Coral Aggregate Concrete

As part of this study, has been developed a numerical method which allows to establish abacuses connecting the normal force with bending moment for a circular section and therefore to predict the rupture of this type of section. This may be for reinforced concrete (traditional steel) or concrete reinforced with steel fibers. The numerical simulation was performed in nonlinear elasticity up to exhaustion of the bearing capacity of the section. The rupture modes considered occur by plasticization of the steel or rupture of the concrete (under compressive stresses or tensile stresses). Regarding the fiber-reinforced concrete, the rupture occurs, usually, by tearing of the fibers. The behavior laws of the different materials (concrete and steel) correspond to the real behavior. The influence of several parameters was investigated, namely; diameter of the section, concrete strength, type of steel, percentage of reinforcement and contribution of concrete in tension between two successive cracks of bending. A comparison was made with the behavior of a section considering the conventional diagrams of materials; provided by the BAEL rules. A second comparative study was performed for fibers reinforced section

An overview of digital speech watermarking

Digital speech watermarking is a robust way to hide and thus secure data like audio and video from any intentional or unintentional manipulation through transmission. In terms of some signal characteristics including bandwidth, voice/non-voice and production model, digital speech signal is different from audio, music and other signals. Although, various review articles on image, audio and video watermarking are available, there are still few review papers on digital speech watermarking. Therefore this article presents an overview of digital speech watermarking including issues of robustness, capacity and imperceptibility. Other issues discussed are types of digital speech watermarking, application, models and masking methods. This article further highlights the related challenges in the real world, research opportunities and future works in this area, yet to be explored fully