Scavenger receptor B1 facilitates the endocytosis of \u3ci\u3eEscherichia coli\u3c/i\u3e via TLR4 signaling in mammary gland infection

SCARB1 belongs to class B of Scavenger receptors (SRs) that are known to be involved in binding and endocytosis of various pathogens. SRs have emerging role in regulating innate immunity and host–pathogen interactions by acting in co-ordination with Toll-like receptors.Query Little is known about the function of SCARB1 in milk-derived mammary epithelial cells (MECs). This study reports the role of SCARB1 in infection and its potential association in TLR4 signaling on bacterial challenge in Goat mammary epithelial cells (GMECs). The novelty in the establishment of MEC culture lies in the method that aims to enhance the viability of the cells with intact characteristics upto a higher passage number. We represent MEC culture to be used as a potential infection model for deeper understanding of animal physiology especially around the mammary gland. On E.coli challenge the expression of SCARB1 was signifcant in induced GMECs at 6 h. Endoribonuclease-esiRNA based silencing of SCARB1 affects the expression of TLR4 and its pathways i.e. MyD88 and TRIF pathways on infection. Knockdown also affected the endocytosis of E.coli in GMECs demonstrating that E.coli uses SCARB1 function to gain entry in cells. Furthermore, we predict 3 unique protein structures of uncharacterized SCARB1 (Capra hircus) protein. Overall, we highlight SCARB1 as a main participant in host defence and its function in antibacterial advances to check mammary gland infections

Mineral Inter-relationship among Soil, forage and Dairy cattle in Kashmir, India

Abstract Samples of soil, forage and blood serum of cattle in six villages Ganderbal district of kashmir were collected and analysed for different macro and micro mineral contents to establish the mineral correlation among soil, forage and animals. The macro and micro mineral contents in soils of Ganderbal district were higher than their respective critical levels except Mn. Similarly, in forage except for zinc all the mineral values were above critical level. The study also revealed the serum mineral values above the critical levels. The soil and forage (r=0.558) and forage and serum (r=0.463) showed significant positive correlations while, non-significant (P<0.05) positive correlation (r=0.08) between soil and serum for Cu was observed. The correlation for Calcium, phosphorus, magnesium, zinc, iron and manganese observed between soil-forage, Forage-serum as well as soil-serum was non-sgnificant. Based on present study, supplementation of calcium, phosphorous, copper and zinc in the diet of cattle under existing feeding practices in Ganderbal district of Kashmir is imperative for better health and productivity. However, further, studies should elucidate the bioavailability and strategic dietary supplementation of minerals for livestoc

​Caprine Respiratory Mycoplasmosis (Contagious Caprine Pleuropneumonia CCPP)- A Global Perspective of the Disease, Epidemiology, Diagnosis, Chemotherapy and Immunization: A Review

Mycoplasma infection of the respiratory tract of goats is prevalent worldwide including the South Asian sub-continent. Owing to intensive and large scale goat farming, the incidence of the disease is on an increase. Among various species of mycoplasma, Mycoplasma capricolum subspecies Capri pneumoniae is increasingly incriminated in Contagious Caprine Pleurapneumonia (CCPP) in goat populations with considerable economic fallout in the form of high morbidity and mortality. The disease manifestations in caprines are recorded as anorexia, high febrile reaction and respiratory embarrassment in the shape of clinical dyspnoea, polypnea, paroxysmal cough and sero-purulent nasal discharges. The disease is thus contracted by the healthy animals through aerosol, contaminated feed and water sources in the herd premises, without a protective immunity and that the conferred immunity in recovered cases being short-lived. The true lesions of CCPP are confined to the lung alveolar tissues of infected goats, which distinguish it from other respiratory diseases of small ruminants caused by the members of the Mycoplasma mycoides cluster. Atypical pneumonia caused by the mycoplasma infection of goats, also known as Contagious Caprine Pleuropneumonia (CCPP) has been more often reported from Africa and Asia than Europe. Classical, acute CCPP attributed to Mycoplasma capricolum subsp. Capri pneumoniae, originally known asthe F38 biotype (World Organisation for Animal Health, 2008) causes heavy kid mortality. Two other organisms in this group, M. mycoides subsp. capri and M. mycoides subsp. mycoides large-colony type, can cause disease in small ruminants that clinico-pathologically mimics CCPP but may have extra pulmonary signs and lesions, sometimes. Mycoplasma Capri pneumoniae and other members of the M. mycoides cluster cross-react in serological tests and share biochemical and genetic similarities. The most favourable epidemiological scenario in the Sub-continent is the hot humid climate during monsoons. The diversity and multi-etiological subspecies involved in the disease is detrimental in the development of an effective vaccine even though in some places a liquid vaccine is presently in use. At other places, anti-mycoplasmal antibiotics of aminoglycoside and fluoroquinolone and perhaps the macrolide groups remain to be the main option in preventing flock mortalities. </jats:p

Mineral Inter-relationship among Soil, forage and Dairy cattle in Kashmir, India

Samples of soil, forage and blood serum of cattle in six villages Ganderbal district of kashmir were collected and analysed for different macro and micro mineral contents to establish the mineral correlation among soil, forage and animals. The macro and micro mineral contents in soils of Ganderbal district were higher than their respective critical levels except Mn. Similarly, in forage except for zinc all the mineral values were above critical level. The study also revealed the serum mineral values above the critical levels. The soil and forage (r=0.558) and forage and serum (r=0.463) showed significant positive correlations while, non-significant (P&lt;0.05) positive correlation (r=0.08) between soil and serum for Cu was observed. The correlation for Calcium, phosphorus, magnesium, zinc, iron and manganese observed between soil-forage, Forage-serum as well as soil-serum was non-sgnificant. Based on present study, supplementation of calcium, phosphorous, copper and zinc in the diet of cattle under existing feeding practices in Ganderbal district of Kashmir is imperative for better health and productivity. However, further, studies should elucidate the bioavailability and strategic dietary supplementation of minerals for livestock [Vet. World 2011; 4(12.000): 550-553

A QoS Aware Cluster Head Selection and Hybrid Cryptography Routing Protocol for Enhancingm Efficiency and Security of VANET

Nowadays, VANET (Vehicular Ad hoc Network) is one of the key aspects of developing advanced intelligent transportation systems. Due to its huge mobility and rapid topology alteration, the network exposes to link failure that affects the firmness of the network and causes delay and congestion. Additionally, the dynamic change in the network routing affects the network’s security, making it vulnerable to various attacks, and results data loss. An efficient and highly secured routing protocol is needed to overcome these drawbacks. Subsequently, this research proposes a new routing protocol that combines the Quality of Service (QoS)-aware Cluster Head (CH) selection and hybrid cryptography named QoS+. The QoS+ protocol is mainly divided into QoS-based CH selection and hybrid cryptography modules. The CH selection module based on QoS parameters attempts to provide reliable and stable clusters and improve the firmness and connectivity during the communication process of the network. The hybrid cryptography module contains Advanced Encryption Standard (AES) and Elliptic Curve Cryptosystems (ECC) algorithms. It attempts to improve the security and privacy of the network. The QoS+ protocol is evaluated by a developed VANET simulator using NS2 software. The simulator consists of a network model, a load model, and an attack model. Various speed and transmission ranges and gray hole and wormhole attacks are used in the simulator. The outcome calculated from the performance analysis shows that the proposed QoS+ protocol has a 7% to 24% higher message success rate, 500 to 800 higher packets normalized routing load, 350 to 550 Kbps higher throughput, 5% to 17% higher efficiency, and 50ms to 12ms lower end-to-end delay when compared with the earlier works of ECHS and KMSUNET. The proposed QoS+ protocol also achieves superior performance in terms of CH efficiency, cluster member efficiency, and average cluster number with various speeds and transmission ranges