THE OPTIMIZATION OF EXTRACTION AND HPLC ANALYSIS OF VITAMINS B FROM YEAST PRODUCTS

This paper describes the optimization of extraction, purification and a simple high performance liquid chromatography with Photodiode Array (PDA) detector method for the simultaneous determination of five water-soluble vitamins. The water-soluble vitamins were analyzed by HPLC on a Restek Ultra Aqueous C18 column (250×4.6 mm, 5 m). All five water-soluble vitamins were separated in a single HPLC run within less than 25 min. The optimization of the HPLC separation was made using pure standards. The extraction and purification of vitamins B was tested from yeast products. Comparative HPLC fingerprint chromatograms of the samples tested were done to demonstrate that water extracts contains only small quantities of free vitamins (B3), meanwhile the hydrolyzed extracts gave different fingerprints. The acidic hydrolyzed extract was poor in vitamins while the alkaline extract contained all 5 vitamins in high concentration

The SWAP EUV Imaging Telescope Part I: Instrument Overview and Pre-Flight Testing

The Sun Watcher with Active Pixels and Image Processing (SWAP) is an EUV solar telescope on board ESA's Project for Onboard Autonomy 2 (PROBA2) mission launched on 2 November 2009. SWAP has a spectral bandpass centered on 17.4 nm and provides images of the low solar corona over a 54x54 arcmin field-of-view with 3.2 arcsec pixels and an imaging cadence of about two minutes. SWAP is designed to monitor all space-weather-relevant events and features in the low solar corona. Given the limited resources of the PROBA2 microsatellite, the SWAP telescope is designed with various innovative technologies, including an off-axis optical design and a CMOS-APS detector. This article provides reference documentation for users of the SWAP image data.Comment: 26 pages, 9 figures, 1 movi

FUNCTIONAL DIETETIC CANDIES WHICH VALORIZE IN A INNOVATIVE WAY THE BEER YEAST WASTE

Nowadays, a great interest is showed on natural phyto-pharmaceuticals with functional proprieties and effects on metabolic syndrome is revealed in bio-medicine. Encapsulation is a new technology which store and stabilize bioactive molecules on different matrices and offer controlled delivery in target tissues and cells. Our studies are focused on dry yeast as waste from secondary fermentation of beer to be valorized as natural bioactive matrix which can incorporate different antioxidant pigments, flavors or can be included in a polymeric mass of low digestible carbohydrates like Isomalt. Starting from a dry beer yeast with controlled composition in proteins, B vitamins, phenolics using HPLC-PDA, we tried different variants of encapsulation into a melted isomalt mass. We’ve added beer yeast powder, cinnamon, lecithin and cocoa extracts, ingredients that are hypoglycemic, antioxidants and can be recommended in metabolic disorders. The HPLC analysis of final product showed the recovery rates of 60-70% of bio-active molecules and their functionality

Engaging Residents in Policy and Planning for Sea Level Rise: Application of the Action-Oriented Stakeholder Engagement for a Resilient Tomorrow (ASERT) Framework

This chapter describes the application of the Action-oriented Stakeholder Engagement for a Resilient Tomorrow (ASERT) framework for communicating with and engaging both residents and community stakeholders in their localities’ efforts to prepare for and to respond to flooding and sea level rise. The application of ASERT incorporates communication, education/learning, and gamification elements that can be embedded into community meetings. We describe the way in which ASERT community meetings are designed (1) to provide an inclusive and engaging process that will allow residents to participate in their city’s resilience efforts; (2) to provide information about resilience in an environment that encourages social learning, including curiosity and reflection, to promote behavioral change that will result in improved resilience and public support for resilience solutions; and (3) to allow residents to offer real-time perceptions of risk to, and feedback about, resilience solutions in their communities and/or cities. This chapter briefly discusses the ASERT framework, illustrates its application (using two examples from Virginia Beach, Virginia), describes the use of gamification in the community meetings, and provides lessons learned regarding communication and stakeholder engagement efforts targeted at building resilience in coastal communities

Cricket and yellow mealworm powders promote higher bioaccessible fractions of mineral elements in functional bread

The application of cricket and yellow mealworm powders to improve the mineral bioaccessibility of bread was investigated. Breads enriched with 10% cricket (CPB-10%) and 10% yellow mealworm (YMPB-10%) powders showed a 1.5-, 2.95-, and 1.22-fold increase in proteins, total lipids, and fibers, respectively, compared to the reference white wheat bread (WFB-100%). Compared to reference bread, a significant increase in the essential amino acids valine (9.72%) and tyrosine (1.86%) contents was observed in the CPB-10% and YMPB-10%. The MUFAs account for 35.22% in CPB-10%, 30.77% in YMPB-10%, and 32.34% in WFB-100%. In vitro digestion experiments showed a higher bioaccessibility of Na, K, Ca, Mg, P, Fe, Zn, Mn, and Li from insect bread than from white bread. Only Cu was more bioaccessible from FB-100% than from insect bread. The results shed light on the possible contribution of insect bread consumption to mitigate deficiencies in several important macro- and microelements

COMPARATIVES TECHNOLOGIES FOR DIETETIC CANDIES PRODUCTION

The production of dietetic candies using isomalt as sugar substitute and some natural ingredients was carried out on a classical technological line with small batch of 25 kg. The caramel mass obtained by replacing sugar with isomalt, was characterized from rheological, sensorial, physical – chemical point of view. The improvement of nutritional value was made by adding natural ingredients on the classical technological line of sugar and glucose syrup candies production by stamping the candy mass. Based on the comparative study of the mentioned parameters, it can be state that the free sugar, but with natural ingredients candies are difficult to produced using the classical technology due to the differences between the rheological and physical – chemical properties of those candies compared with sugar candies. In our study, we produced the caramel mass on the same under vacuum boiling system, but the candy formation was made by casting or extrusio

Development and pilot implementation of a novel protocol to assess capacity and readiness of health systems to adopt HPV detection-based cervical cancer screening in Europe

BACKGROUND: Cervical cancer remains a significant public health concern in Europe. Effective introduction and scaling up of human papillomavirus (HPV) detection-based cervical cancer screening (CCS) requires a systematic assessment of health systems capacity. However, there is no validated capacity assessment methodology for CCS programmes, especially in European contexts. Addressing this gap, our study introduces an innovative and adaptable protocol for evaluating the capacity of CCS programmes across varying European health system settings. METHODS: Our research team developed a three-step capacity assessment framework, incorporating a health policy review checklist, a facility visit survey, and key informants' interview guide followed by a strengths, weaknesses, opportunities and threats (SWOT) analysis. Piloting this comprehensive approach, we explored the CCS capacity in three countries: Estonia, Portugal and Romania. These countries were selected due to their contrasting healthcare structures and resources, providing a diverse overview of the European context. RESULTS: Conducted over a period of 9 months, the capacity assessment covered multiple resources, 27 screening centres, 16 colposcopy and treatment centres and 15 key informant interviews. Our analysis highlighted both shared and country-specific challenges. A key common issue was ensuring high compliance to follow-up and management of screen-positive women. We identified considerable heterogeneity in resources and organization across the three countries, underscoring the need for tailored, rather than one-size-fits-all, solutions. CONCLUSIONS: Our study's novelty lies in the successful development of this capacity assessment methodology implementable within a relatively short time frame, proving its feasibility for use in various contexts and countries. The resulting set of materials, adaptable to different cancer types, is a ready-to-use toolkit to improve cancer screening processes and outcomes. This research marks a significant stride towards comprehensive capacity assessment for CCS programmes in Europe. Future directions include deploying these tools in other countries and cancer types, thereby contributing to the global fight against cancer. © 2024. The Author(s).CBIG-SCREEN has received funding from the EU Horizon 2020 Research and Innovation Programme under grant agreement no. 964049. Official starting date: 1 March 2021

Participatory GIS as a Tool for Stakeholder Engagement in Building Resilience to Sea Level Rise: A Demonstration Project

This article describes a participatory geographical information system (PGIS) demonstration project used as part of the stakeholder engagement efforts undertaken by the Citizen Engagement Working Group of the Hampton Roads Sea Level Rise Preparedness and Resilience Intergovernmental Planning Pilot Project. The PGIS demonstration project was conducted in the Little Creek/Pretty Lake case study area in the Hampton Roads region of southeastern coastal Virginia. PGIS served as a deliberative and participatory mechanism to obtain local knowledge from residents about the location of valued assets within the community and locations challenged by increased flooding and sea level rise. The PGIS application, using the weTable tool, was found to be useful for soliciting and documenting local knowledge, such as by highlighting community assets and identifying community challenges. It was also found to be useful for facilitating community-wide discussion, visualizing the problem, and understanding the severity of sea level rise and flooding. The PGIS demonstration project showed how participatory mapping can directly engage residents in creating sociospatial data, build knowledge, and foster learning and deliberation in a complex issue such as resilience to flooding and sea level rise

The SWAP Filter: A Simple Azimuthally Varying Radial Filter for Wide-Field EUV Solar Images

We present the SWAP Filter: an azimuthally varying, radial normalizing filter specifically developed for EUV images of the solar corona, named for the Sun Watcher with Active Pixels and Image Processing (SWAP) instrument on the Project for On-Board Autonomy 2 spacecraft. We discuss the origins of our technique, its implementation and key user-configurable parameters, and highlight its effects on data via a series of examples. We discuss the filter's strengths in a data environment in which wide field-of-view observations that specifically target the low signal-to-noise middle corona are newly available and expected to grow in the coming years.Comment: Contact D. B. Seaton for animations referenced in figure caption