Probabilistic boundary element method

The purpose of the Probabilistic Structural Analysis Method (PSAM) project is to develop structural analysis capabilities for the design analysis of advanced space propulsion system hardware. The boundary element method (BEM) is used as the basis of the Probabilistic Advanced Analysis Methods (PADAM) which is discussed. The probabilistic BEM code (PBEM) is used to obtain the structural response and sensitivity results to a set of random variables. As such, PBEM performs analogous to other structural analysis codes such as finite elements in the PSAM system. For linear problems, unlike the finite element method (FEM), the BEM governing equations are written at the boundary of the body only, thus, the method eliminates the need to model the volume of the body. However, for general body force problems, a direct condensation of the governing equations to the boundary of the body is not possible and therefore volume modeling is generally required

Identification of New Drug Candidates Against \u3cem\u3eBorrelia burgdorferi\u3c/em\u3e Using High-Throughput Screening

Lyme disease is the most common zoonotic bacterial disease in North America. It is estimated that .300,000 cases per annum are reported in USA alone. A total of 10%–20% of patients who have been treated with antibiotic therapy report the recrudescence of symptoms, such as muscle and joint pain, psychosocial and cognitive difficulties, and generalized fatigue. This condition is referred to as posttreatment Lyme disease syndrome. While there is no evidence for the presence of viable infectious organisms in individuals with posttreatment Lyme disease syndrome, some researchers found surviving Borrelia burgdorferi population in rodents and primates even after antibiotic treatment. Although such observations need more ratification, there is unmet need for developing the therapeutic agents that focus on removing the persisting bacterial form of B. burgdorferi in rodent and nonhuman primates. For this purpose, high-throughput screening was done using BacTiter-Glo assay for four compound libraries to identify candidates that stop the growth of B. burgdorferi in vitro. The four chemical libraries containing 4,366 compounds (80% Food and Drug Administration [FDA] approved) that were screened are Library of Pharmacologically Active Compounds (LOPAC1280), the National Institutes of Health Clinical Collection, the Microsource Spectrum, and the Biomol FDA. We subsequently identified 150 unique compounds, which inhibited .90% of B. burgdorferi growth at a concentration of ,25 µM. These 150 unique compounds comprise many safe antibiotics, chemical compounds, and also small molecules from plant sources. Of the 150 unique compounds, 101 compounds are FDA approved. We selected the top 20 FDA-approved molecules based on safety and potency and studied their minimum inhibitory concentration and minimum bactericidal concentration. The promising safe FDA-approved candidates that show low minimum inhibitory concentration and minimum bactericidal concentration values can be chosen as lead molecules for further advanced studies

Estuarine and Marine Decapods of Karnataka Inventory

The inventory of estuarine and marine decapods of Karnataka State of India has been prepared. This list has 42 species of shrimps/prawns, 112 species of brachyuran crabs, 14 species of hermit crabs and nine species of lobsters, collected from eight estuaries and coastal/ offshore waters of Karnataka State

Assessment of bycatch and discards associated with bottom trawling along Karnataka coast, India

The quantity of bycatch and discard was estimated from the landings of multiday fishing trawlers (MDF) at fisheries harbours and using the data collected onboard single-day fishing trawlers (SDF) for the period 2001-02. The quantity of bycatch generated by trawling along Kamataka coast was estimated as 56,035 tin 2001 and 52,380 t in 2002 forming 54 % and 48 % of total trawl catch respectively

Computational modeling of failure in composite structures including uncertainties in material and geometrical properties

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/90654/1/AIAA-2011-1722-752.pd

Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy

We report here the design, synthesis and application of pyrene binary oligonucleotide probes for selective detection of cellular mRNA. The detection strategy is based on the formation of a fluorescent excimer when two pyrene groups are brought into close proximity upon hybridization of the probes with the target mRNA. The pyrene excimer has a long fluorescence lifetime (>40 ns) compared with that of cellular extracts (∼7 ns), allowing selective detection of the excimer using time-resolved emission spectra (TRES). Optimized probes were used to target a specific region of sensorin mRNA yielding a strong excimer emission peak at 485 nm in the presence of the target and no excimer emission in the absence of the target in buffer solution. While direct fluorescence measurement of neuronal extracts showed a strong fluorescent background, obscuring the detection of the excimer signal, time-resolved emission measurements indicated that the emission decay of the cellular extracts is ∼8 times faster than that of the pyrene excimer probes. Thus, using TRES of the pyrene probes, we are able to selectively detect mRNA in the presence of cellular extracts, demonstrating the potential for application of pyrene excimer probes for imaging mRNAs in cellular environments that have background fluorescence

Analysis of Five-Level DSTATCOM for Harmonic Reduction in Power System Due To Non-Linear Loads

This paper deals with the elimination of harmonics at PCC in the presence of non-linear type of loads. Introduction of five-level DSTATCOM at PCC injects compensating currents for the minimization of harmonic content in the source currents. Non-linear loads when connected to the system draw non-linear components of current leaving undesired harmonics at the source side. DSTATCOM when connected to the system at the coupling point induces compensating currents to nullify harmonics. This paper introduces the simulation modelling of five-level DSTATCOM for harmonic elimination. Matlab/Simulink  modelling was done for the system without DSTATCOM and its harmonic contents were shown. The system without DSTATCOM is compared with the system with the presence of DSTATCOM showing their respective THD in the source current. Five level DSTATCOM was validated by considering fixed load and variable load. Simulation work was carried out for three types of systems and results were obtained using Matlab/Simulink

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PREPARATION AND EVALUATION OF QUETIAPINE FUMARATE MICROEMULSIONS: A NOVEL DELIVERY SYSTEM

Objective: In the present study, the main objective is to improve solubility and bioavailability of Quetiapine fumarate by formulation into micro emulsion. Method: The Quetiapine fumarate micro emulsion was formulated by using mixture of Isopropyl myristate and oleic acid as oil phase, Tween-80 as surfactant, Isopropyl alcohol and Ethanol mixture as co-surfactant by phase titration method. The prepared formulations were evaluated for Limpidity (% transmittance), droplet size, Zeta potential, Electrical conductivity, Rheology, pH, percentage of drug (assay), emulsifying time, in vitro drug diffusion studies and ex vivo permeation studies. Results and conclusion: The Optimized micro emulsion (Micro emulsion 11) formulation containing Quetiapine fumarate (25mg), Surfactant mixture (50%w/w), Oil (12%w/w) and distilled water (38%w/w) has a droplet size of 26.70 nm with a zeta potential of -5.62 millivolts. The micro emulsion was characterized and compared with the pure drug suspension. Microemulsion showed 31.25 fold increased solubility than that of pure drug suspension. In vitro drug release and ex vivo permeation study results were comparable and correlative. The Microemulsion 11 formulation showed 1.4763 times more drug release than that of pure drug suspension. The formulation was found to be stable for three months. Keywords: Microemulsion, Phase titration method, Quetiapine Fumarate,  Emulsifying time

Effect of Plant Growth Regulators on Growth and Yield of Daisy (Aster amellus L.) Cv. Dwarf Pink

An experiment was carried out to improve plant stature in daisy (Aster amellus L.) cv. Dwarf Pink using various growth regulators. Among the various growth promoters and their combinations tested, gibberellic acid (150ppm) and brassinosteroid (0.5ppm) application produced maximum vegetative growth, spike yield and vase-life. It is concluded that a combination of gibberellic acid (150ppm) and BR (0.5ppm) is helpful improving growth and quality in daisy cv. Dwarf Pink