A cellulose-based bioassay for the colorimetric detection of pathogen DNA

Cellulose-paper-based colorimetric bioassays may be used at the point of sampling without sophisticated equipment. This study reports the development of a colorimetric bioassay based on cellulose that can detect pathogen DNA. The detection was based on covalently attached single-stranded DNA probes and visual analysis. A cellulose surface functionalized with tosyl groups was prepared by the N,N-dimethylacetamide-lithium chloride method. Tosylation of cellulose was confirmed by scanning electron microscopy, Fourier transform infrared spectroscopy and elemental analysis. Sulfhydryl-modified oligonucleotide probes complementary to a segment of the DNA sequence IS6110 of Mycobacterium tuberculosis were covalently immobilized on the tosylated cellulose. On hybridization of biotin-labelled DNA oligonucleotides with these probes, a colorimetric signal was obtained with streptavidin-conjugated horseradish peroxidase catalysing the oxidation of tetramethylbenzamidine by H2O2. The colour intensity was significantly reduced when the bioassay was subjected to DNA oligonucleotide of randomized base composition. Initial experiments have shown a sensitivity of 0.1 μM. A high probe immobilization efficiency (more than 90 %) was observed with a detection limit of 0.1 μM, corresponding to an absolute amount of 10 pmol. The detection of M. tuberculosis DNA was demonstrated using this technique coupled with PCR for biotinylation of the DNA. This work shows the potential use of tosylated cellulose as the basis for point-of-sampling bioassays.Peer reviewedFinal Accepted Versio

Mechanisms underlying extremely fast muscle V˙O2 on-kinetics in humans.

The time constant of the primary phase of pulmonary V˙O2 on-kinetics (τp ), which reflects muscle V˙O2 kinetics during moderate-intensity exercise, is about 30 s in young healthy untrained individuals, while it can be as low as 8 s in endurance-trained athletes. We aimed to determine the intramuscular factors that enable very low values of t0.63 to be achieved (analogous to τp , t0.63 is the time to reach 63% of the V˙O2 amplitude). A computer model of oxidative phosphorylation (OXPHOS) in skeletal muscle was used. Muscle t0.63 was near-linearly proportional to the difference in phosphocreatine (PCr) concentration between rest and work (ΔPCr). Of the two main factors that determine t0.63 , a huge increase in either OXPHOS activity (six- to eightfold) or each-step activation (ESA) of OXPHOS intensity (>3-fold) was needed to reduce muscle t0.63 from the reference value of 29 s (selected to represent young untrained subjects) to below 10 s (observed in athletes) when altered separately. On the other hand, the effect of a simultaneous increase of both OXPHOS activity and ESA intensity required only a twofold elevation of each to decrease t0.63 below 10 s. Of note, the dependence of t0.63 on OXPHOS activity and ESA intensity is hyperbolic, meaning that in trained individuals a large increase in OXPHOS activity and ESA intensity are required to elicit a small reduction in τp . In summary, we postulate that the synergistic action of elevated OXPHOS activity and ESA intensity is responsible for extremely low τp (t0.63 ) observed in highly endurance-trained athletes

Towards an Improved Gain Scheduling Predictive Control Strategy for a Solar Thermal Power Plant

This paper improves a recently proposed gain scheduling predictive control strategy for the ACUREX distributed solar collector field at the Plataforma Solar de Almería, in south-east Spain. Measured disturbances are an integral part of the plant and while simple classical, series and parallel, feedforward approaches have been proposed and used extensively in the literature, the proposed approach incorporates a feedforward systematically into the predictive control strategy by including the effects of the measured disturbances of the ACUREX plant into the predictions of future outputs. Models of the measured disturbances are estimated around a family of operating points directly from input-output data and using a subspace identification method while taking into account the frequency response of the plant. Input-output data are obtained from a validated nonlinear simulation model of the plant rather than the plant itself. The nonlinear simulation model is validated here against measured data obtained from the ACUREX plant and the effectiveness of the proposed control approach is evaluated in the same nonlinear simulation environment. The paper also considers related issues like the significance of sufficient modelling of the measured disturbances of the ACUREX plant and the impact of incorporating the expected future behaviour of a measured disturbance along a given prediction horizon, a theme which has received little attention in the literature

Wat onthoudt een consument van een tv-commercial? Een kijkje in het brein met neuro-imaging technieken

Met een nieuwe neuro-imaging techniek om de activiteit in de hersenen te meten, de zogenaamde steady-state probe topography (SSPT), kan opgespoord worden welke scènes uit een tv-commercial door consumenten goed herinnerd worden. Uit een experiment blijkt dat scènes die langer dan 1,5 seconde duren en scènes die een snelle activatie in de linkerhersenhelft veroorzaken, een week later beter herkend worden. Door het toepassen van SSPT kunnen commercials gepretest worden om de herkenning en herinnering zo hoog mogelijk te maken

Input shaping for PFC: how and why?

Predictive functional control (PFC) is a highly successful strategy within industry, but for cases with challenging dynamics the most effective tuning approaches are still an active research area. This paper shows how one can deploy some insights from the more traditional model predictive control literature in order to enable systematic tuning and in particular, to ensure that the key PFC tuning parameter, that is the desired closed-loop time constant, is effective. In addition to enabling easier and more effective tuning, the proposed approach has the advantage of being simple to code and thus retaining the simplicity of implementation and tuning that is a key selling point of PFC. This paper focuses on design for open-loop unstable and also processes with significant under-damping in their open-loop behaviour

Surface electrical properties experiment, part 1

The work is reported which was performed on the Surface Electrical Properties Experiment Data Acquisition System. Areas discussed include: data handling and processing, installation and external signal application, operation of the equipment, and digital output. Detailed circuit descriptions are included

Temperature dependence of the resistance of metallic nanowires (diameter ≥\geq 15 nm): Applicability of Bloch-Gr\"{u}neisen theorem

We have measured the resistances (and resistivities) of Ag and Cu nanowires of diameters ranging from 15nm to 200nm in the temperature range 4.2K-300K with the specific aim to assess the applicability of the Bloch-Gr\"{u}neisen formula for electron phonon resistivity in these nanowires. The wires were grown within polymeric templates by electrodeposition. We find that in all the samples the resistance reaches a residual value at T=4.2K and the temperature dependence of resistance can be fitted to the Bloch-Gr\"{u}neisen formula in the entire temperature range with a well defined transport Debye temperature ($\Theta_{R}$). The value of Debye temperature obtained from the fits lie within 8% of the bulk value for Ag wires of diameter 15nm while for Cu nanowires of the same diameter the Debye temperature is significantly lesser than the bulk value. The electron-phonon coupling constants (measured by $\alpha_{el-ph}$ or $\alpha_{R}$) in the nanowires were found to have the same value as that of the bulk. The resistivities of the wires were seen to increase as the wire diameter was decreased. This increase in the resistivity of the wires may be attributed to surface scattering of conduction electrons. The specularity p was estimated to be about 0.5. The observed results allow us to obtain the resistivities exactly from the resistance and gives us a method of obtaining the exact numbers of wires within the measured array (grown within the template).Comment: 9 pages, 10 figure