Changes in serum levels of quinolones in rats under the influence of experimental trauma

Administration of antibiotics is considered an important factor during, or after, operational procedures in the maxillofacial area, in order to avoid post-surgical complications. In the present study, the levels of quinolones in serum and tissues such as the parotid gland, the tongue and the bone of the jaws were estimated during traumatic injury in the oral cavity. For this purpose, two groups (A and B) of Wistar rats, consisting of 35 animals each were used: Group A (control) and group B (experimental) were divided in five subgroups (A1, A2, A3, A4, A5 and B1, B2, B3, B4, B5). In the experimental group, model traumatic injury was performed through the Whole length of the cheek. Subjects received orally ciprofloxacin, pefloxacin, norfloxacin, ofloxacin and cinoxacin: The concentration of quinolones in serum and in most of the tissues was significantly higher in the experimental groups than in controls. In addition, the FFA levels and the weight of adrenals (as indicators of stress) were higher in the trauma groups: Stress seemed to affect many pathophysiological mechanisms which are responsible for the alterations observed

Does stress influence ampicillin concentration in serum and tissues?

Exercise produces changes of drug levels in plasma and increases the concentration of free fatty acids (FFAs), which may interfere with drug-protein binding. FFAs seem to play an antagonistic role to drugs since they have a strong binding capacity to serum albumin. The aim of this study was to evaluate the influence of the consecutive exercise-induced stress in ampicillin levels. Two groups of Wistar rats were used. Group A consisted of six subgroups that were subjected to cold swimming (4°C) for 5, 10, 15, 20, 25, 30 days respectively. Group B was the control group. The animals were injected im. with ampicillin (1 g/Kg/8h in 5 doses). Results showed that exercise enhanced stress parameters (FFAs, adrenal weight, Ht%) and led to an ampicillin increase in all experimental groups comparatively to controls