Pulso mínimo de agua para la emergencia de plántulas de tres especies de gramíneas perennes nativas del Monte Central (Argentina), influenciado por el efecto de la sombra y la estación del año

In deserts, seedling emergence occurs only after precipitation threshold has been exceeded, however, the presence of trees modifies microenvironmental conditions that might affect the effectiveness of a water pulse. In the Monte desert, Prosopis flexuosa trees generate different micro-environmental conditions that might influence grass seedlings establishment. The objective of this work was: a) to know the effective minimum water input event that triggers the emergence of native perennial grass seedlings; b) to relate this fact with the effect of the shade of P. flexuosa canopy and the seasonal temperatures. Three important forage species of the Monte were studied: Pappophorum caespitosum and Trichloris crinita, with C4, and Jarava ichu, with C3 metabolism. Each season, seeds of these species were sown in pots placed at two light conditions: shade (similar to P. flexuosa cover) and open area, and with seven irrigation treatments (0, 10, 20, 30, 40, 2*10 and 3*10 mm). J. ichu did not emerge in any of the treatments. Significant seedling emergence was registered for P. caespitosum and T. crinita in shade conditions with 40 mm irrigation treatment in summer. Since 40 mm precipitation events are infrequent in the Monte, seedling emergence for these species would be restricted to exceptional rainy years. The facilitating effect of P. flexuosa shade would be important during the hot season.En los desiertos, la emergencia de las plántulas se produce cuando un evento de precipitación excede un valor umbral. La presencia de árboles modifica las condiciones micro ambientales y en consecuencia, la efectividad de los eventos de lluvia. En el Monte, las condiciones generadas por Prosopis flexuosa (algarrobo) afectarían el establecimiento de las gramíneas. El objetivo del trabajo fue: a) conocer el evento mínimo de agua necesario para desencadenar la emergencia de gramíneas perennes; b) relacionarlo con la influencia de la sombra del algarrobo y las temperaturas estacionales. Se trabajó con tres especies nativas de gramíneas forrajeras perennes: Pappophorum caespitosum, Trichloris crinita, (C4), y Jarava ichu (C3). En cada estación del año, semillas de las tres especies fueron sembradas en macetas bajo dos condiciones lumínicas: sombra (similar a la sombra de P. flexuosa) y área abierta y con siete niveles de riego (0, 10, 20, 30, 40, 2*10 y 3*10 mm). Jarava ichu no emergió en ningún tratamiento. En el verano se obtuvo emergencia significativa de T. crinita y P. caespitosum solo en los tratamientos de 40 mm y a la sombra. Debido a que en el Monte los eventos de precipitación de 40 mm son infrecuentes, la emergencia de estas especies ocurriría en los años excepcionalmente lluviosos. El efecto facilitador de la sombra del algarrobo sería de importancia en la estación cálida.Fil: Greco, Silvina A.. Universidad Nacional de Cuyo. Facultad de Ciencias AgrariasFil: Sartor, Carmen E.. Universidad Nacional de Cuyo. Facultad de Ciencias AgrariasFil: Villagra, Pablo E.. Universidad Nacional de Cuyo. Facultad de Ciencias Agraria

Comunicación de RSC: una revisión de las tesis clásicas sobre la coherencia entre la acción de RSC y la actividad organizacional

La literatura sobre comunicación eficaz de la Responsabilidad Social Corporativa (RSC) es compleja y, en ocasiones, contradictoria respecto al papel de la coherencia entre la actividad de la empresa y las acciones de RSC (fit empresarial clásico). Por una parte, subraya la importancia del fit para una comunicación eficaz. Sin embargo, otros investigadores afirman que el fit podría aumentar el escepticismo y resultar perjudicial. Por último, investigaciones recientes señalan otro tipo de fit: el fit personal, donde lo importante es la coincidencia entre la acción de RSC y los intereses del público. Para intentar esclarecer esta complejidad se llevó a cabo un experimento en el que 86 jóvenes fueron asignados aleatoriamente a tres condiciones experimentales: mensaje de RSC con fit clásico; mensaje de RSC con fit personal y mensaje control. A diferencia de lo que se podría esperar a partir de la literatura previa, no se encontraron diferencias significativas entre los que recibieron el mensaje con fit empresarial y aquellos que recibieron el mensaje control. Por el contrario, aquellos individuos asignados a la condición de fit personal evaluaron a la empresa como más sincera y honesta y manifestaron una intención conductual más favorable hacia ella que los sujetos asignados a la condición de control y que los sujetos asignados a la condición de fit empresarial. El artículo discute la necesidad de replantear el rol del fit clásico empresarial en la comunicación de acciones de RSC y resalta la existencia de otros tipos de fit que pueden ser incluso más determinantes

Boletín NUESTRA AMÉRICA XXI - Desafíos y alternativas, num.4, febrero 2017

Una excelente iniciativa del Grupo de Trabajo Crisis y economía mundial, coordinado por María Josefina Morales y Gabriela Roffinelli

Essential role of HDAC6 in the regulation of PD-L1 in melanoma

Indexación: Web of Science; Scopus.Histone deacetylases (HDACs), originally described as histone modifiers, have more recently been deMonstrated to target a variety of other proteins unrelated to the chromatin environment. In this context, our present work demonstrates that the pharmacological or genetic abrogation of HDAC6 in primary melanoma samples and cell lines, down-regulates the expression of PD-L1, an important co-stimulatory molecule expressed in cancer cells, which activates the inhibitory regulatory pathway PD-1 in T-cells. Our data suggests that this novel mechanism of PD-L1 regulation is mainly mediated by the influence of HDAC6 over the recruitment and activation of STAT3. Additionally, we observed that selective HDAC6 inhibitors impairs tumor growth and reduce the in vim expression of several inhibitory checkpoint molecules and other regulatory pathways involved in immunosurveillance. Most importantly, these results provide a key pre-clinical rationale and justification to further study isotype selective HDAC6 inhibitors as potential immuno-modulatory agents in cancer. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.http://onlinelibrary.wiley.com/doi/10.1016/j.molonc.2015.12.012/abstract;jsessionid=DB86CF943DA7FD358A75C2CEEAD4D7C4.f03t0

Expression of Regulatory Platelet MicroRNAs in Patients with Sickle Cell Disease

Background: Increased platelet activation in sickle cell disease (SCD) contributes to a state of hypercoagulability and confers a risk of thromboembolic complications. The role for post-transcriptional regulation of the platelet transcriptome by microRNAs (miRNAs) in SCD has not been previously explored. This is the first study to determine whether platelets from SCD exhibit an altered miRNA expression profile. Methods and Findings: We analyzed the expression of miRNAs isolated from platelets from a primary cohort (SCD = 19, controls = 10) and a validation cohort (SCD = 7, controls = 7) by hybridizing to the Agilent miRNA microarrays. A dramatic difference in miRNA expression profiles between patients and controls was noted in both cohorts separately. A total of 40 differentially expressed platelet miRNAs were identified as common in both cohorts (p-value 0.05, fold change>2) with 24 miRNAs downregulated. Interestingly, 14 of the 24 downregulated miRNAs were members of three families - miR-329, miR-376 and miR-154 - which localized to the epigenetically regulated, maternally imprinted chromosome 14q32 region. We validated the downregulated miRNAs, miR-376a and miR-409-3p, and an upregulated miR-1225-3p using qRT-PCR. Over-expression of the miR-1225-3p in the Meg01 cells was followed by mRNA expression profiling to identify mRNA targets. This resulted in significant transcriptional repression of 1605 transcripts. A combinatorial approach using Meg01 mRNA expression profiles following miR-1225-3p overexpression, a computational prediction analysis of miRNA target sequences and a previously published set of differentially expressed platelet transcripts from SCD patients, identified three novel platelet mRNA targets: PBXIP1, PLAGL2 and PHF20L1. Conclusions: We have identified significant differences in functionally active platelet miRNAs in patients with SCD as compared to controls. These data provide an important inventory of differentially expressed miRNAs in SCD patients and an experimental framework for future studies of miRNAs as regulators of biological pathways in platelets. © 2013 Jain et al

Prevalence Rates of Mental Disorders in Chilean Prisons

PMCID: PMC371883

Molecular and cellular mechanisms underlying the evolution of form and function in the amniote jaw.

The amniote jaw complex is a remarkable amalgamation of derivatives from distinct embryonic cell lineages. During development, the cells in these lineages experience concerted movements, migrations, and signaling interactions that take them from their initial origins to their final destinations and imbue their derivatives with aspects of form including their axial orientation, anatomical identity, size, and shape. Perturbations along the way can produce defects and disease, but also generate the variation necessary for jaw evolution and adaptation. We focus on molecular and cellular mechanisms that regulate form in the amniote jaw complex, and that enable structural and functional integration. Special emphasis is placed on the role of cranial neural crest mesenchyme (NCM) during the species-specific patterning of bone, cartilage, tendon, muscle, and other jaw tissues. We also address the effects of biomechanical forces during jaw development and discuss ways in which certain molecular and cellular responses add adaptive and evolutionary plasticity to jaw morphology. Overall, we highlight how variation in molecular and cellular programs can promote the phenomenal diversity and functional morphology achieved during amniote jaw evolution or lead to the range of jaw defects and disease that affect the human condition

Statistical support for the hypothesis of developmental constraint in marsupial skull evolution.

Background: In contrast to placental neonates, in which all cranial bones are ossified, marsupial young have only the bones of the oral region and the exoccipital ossified at birth, in order to facilitate suckling at an early stage of development. In this study, we investigated whether this heterochronic shift in the timing of cranial ossification constrains cranial disparity in marsupials relative to placentals. Methods: We collected three-dimensional (3D) landmark data about the crania of a wide range of extant placentals and marsupials, and from six fossil metatherians (the clade including extant marsupials and their stem relatives), using a laser scanner and a 3D digitizer. Principal components analysis and delta variance tests were used to investigate the distribution and disparity of cranial morphology between different landmark sets (optimizing either number of landmarks or number of taxa) of the whole skull and of individual developmental or functional regions (neurocranium, viscerocranium, oral region) for extant placentals and marsupials. Marsupial and placental data was also compared based on shared ecological aspects including diet, habitat, and time of peak activity. Results: We found that the extant marsupial taxa investigated here occupy a much smaller area of morphospace than the placental taxa, with a significantly (P<0.01) smaller overall variance. Inclusion of fossil taxa did not significantly increase the variance of metatherian cranial shape. Fossil forms generally plotted close to or within the realm of their extant marsupial relatives. When the disparities of cranial regions were investigated separately, significant differences between placentals and marsupials were seen for the viscerocranial and oral regions, but not for the neurocranial region. Conclusion: These results support the hypothesis of developmental constraint limiting the evolution of the marsupial skull, and further suggest that the marsupial viscerocranium as a whole, rather than just the early-ossifying oral region, is developmentally constrained

Cranial form differences in goats by breed and domestic status

Domestic goats (Capra hircus) are globally represented by over 300 breeds, making them a useful model for investigating patterns of morphological change related to domestication. However, they have been little studied, likely due to their poor representation in museum collections and the difficulty in obtaining truly wild goat (Capra aegagrus, the bezoar) samples. Similar studies on other species reveal that domestication correlates with craniofacial alterations in domestics, which are non-uniform and often species-specific. Here, we use three-dimensional geometric morphometric methods (3DGMM) to describe and quantify cranial shape variation in wild (n = 21) versus domestic (n = 54) goats. We find that mean cranial shapes differ significantly between wild and domestic goats as well as between certain breeds. The detected differences are lower in magnitude than those reported for other domestic groups, possibly explained by the fewer directions of artificial selection in goat breeding, and their low global genetic diversity compared to other livestock. We also find tooth-row length reduction in the domestics, suggestive of rostral shortening—a prediction of the “domestication syndrome” (DS). The goat model thus expands the array—and combinations of—morphological changes observed under domestication, notably detecting alterations to the calvarium form which could be related to the ~ 15% brain size reduction previously reported for domestic compared to wild goats. The global success of domestic goats is due more to their ability to survive in a variety of harsh environments than to systematized human management. Nonetheless, their domestication has resulted in a clear disruption from the wild cranial form, suggesting that even low-intensity selection can lead to significant morphological changes under domestication