441 research outputs found

    'Workshop for Nagoya Protocol and Plant Treaty National Focal Points in Latin America and the Caribbean’

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    The capacity-building Workshop for National Focal Points in Latin America and the Caribbean on Mutually Supportive Implementation of the Nagoya Protocol and the International Treaty on Plant Genetic Resources for Food and Agriculture, was held 25-28 September 2018 at the International Potato Center (CIP), Lima, Peru. The workshop was attended by over 60 participants, including National Focal Points for the Nagoya Protocol to the Convention on Biological Diversity and the International Treaty on Plant Genetic Resources (CBD) for Food and Agriculture (Plant Treaty), from 16 countries in Latin America and the Caribbean. The workshop was also attended by representatives from the Secretariats of the Plant Treaty and CBD, the International Seed Federation, farmer and indigenous peoples organizations, national and international agricultural research organizations and experts from the region who have been working for decades on access and benefit-sharing policy issues. The objectives of the workshop were to: 1. Strengthen network ties between National Focal Points within each country and across the regions; 2. Analyse challenges and opportunities for implementing the Plant Treaty and the Nagoya Protocol in a mutually supportive manner, and in ways that advance complementary policy goals, such as climate change adaptation, and improving the livelihoods of indigenous peoples and local communities; 3. Equip participants with tools to help address ‘real life’ scenarios where mutually supportive implementation is important, and 4. Identify the kinds of additional support that countries need to implement the Plant Treaty and Nagoya Protocol in mutually supportive ways

    Perception of concept and practice of social power in development interventions in Malawi

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    The study examined research participants’ perception of social power in intervention programmes in Malawi. Two districts and four villages with active participation in the intervention programmes were purposively selected. Focus group participants were purposively identified, while the snow balling procedure was employed to select key informants. A total of 375 participants consisting of 219 men and 156 women (to better explore the viewpoints of men from those of women) were drawn from the two study locations. Data were analysed by content analysis. The results showed that >98% of participants stated that power meant the ‘capacity of a social actor to influence decisions and secure compliance of other social actors. Less than 98% also perceived ‘power’ as the leadership ability of a social actor but few participants with high level of power shared this construct. Further analysis informed that power was perceived as the act of guiding fellow social actors to plan and implement activities serving common interest while another 50% of respondents perceived power as a mere potential ability to influence. Statistics however showed only 3.7% of relatively powerful social actors from agricultural extension workers and 1.3 % of sexual and reproductive health interventionists shared power as potential ability to influence way of thinking and doing. Therefore, stakeholders of development intervention should recognize experienced social actors and traditions as power indicators since these will enhance effective extension policy process aimed at development intervention among rural populace

    Solid state and sub-cooled liquid vapour pressures of substituted dicarboxylic acids using Knudsen Effusion Mass Spectrometry (KEMS) and Differential Scanning Calorimetry

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    Solid state vapour pressures of a selection of atmospherically important substituted dicarboxylic acids have been measured using Knudsen Effusion Mass Spectrometry (KEMS) over a range of 20 K (298–318 K). Enthalpies of fusion and melting points obtained using Differential Scanning Calorimetry (DSC) were used to obtain sub-cooled liquid vapour pressures. They have been compared to estimation methods used on the E-AIM website. These methods are shown to poorly represent – OH groups in combination with COOH groups. Partitioning calculations have been performed to illustrate the impact of the different estimation methods on organic aerosol mass compared to the use of experimental data

    Affective practices, care and bioscience: a study of two laboratories

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    Scientific knowledge-making is not just a matter of experiments, modelling and fieldwork. It also involves affective, embodied and material practices (Wetherell 2012) which can be understood together as 'matters of care' (Puig de la Bellacasa, 2011). In this paper we explore how affect spans and connects material, subjective and organisational practices, focusing in particular on the patterns of care we encountered in an observational study of two bioscience laboratories. We explore the preferred emotional subjectivities of each lab and their relation to material practice. We go on to consider flows and clots in the circulation of affect and their relation to care through an exploration of belonging and humour in the labs. We show how being a successful scientist or group of researchers involves a careful choreography of affect in relation to materials, colleagues and others to produce scientific results, subjects and workplaces. We end by considering how thinking with care troubles dominant constructions of scientific practice, successful scientific selves and collectives

    Utilization of a deoxynucleoside diphosphate substrate by HIV reverse transcriptase

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    Background: Deoxynucleoside triphosphates (dNTPs) are the normal substrates for DNA sysnthesis is catalyzed by polymerases such as HIV-1 reverse transcriptase (RT). However, substantial amounts of deoxynucleoside diphosphates (dNDPs) are also present in the cell. Use of dNDPs in HIV-1 DNA sysnthesis could have significant implications for the efficacy of nucleoside RT inhibitors such as AZT which are first line therapeutics fro treatment of HIV infection. Our earlier work on HIV-1 reverse transcriptase (RT) suggested that the interaction between the γ phosphate of the incoming dNTP and RT residue K65 in the active site is not essential for dNTP insertion, implying that this polymerase may be able to insert dNPs in addition to dNTPs. Methodology/Principal Findings: We examined the ability of recombinant wild type (wt) and mutant RTs with substitutions at residue K65 to utilize a dNDP substrate in primer extension reactions. We found that wild type HIV-1 RT indeed catalyzes incorporation of dNDP substrates whereas RT with mutations of residue K645 were unable to catalyze this reaction. Wild type HIV-1 RT also catalyzed the reverse reaction, inorganic phosphate-dependent phosphorolysis. Nucleotide-mediated phosphorolytic removal of chain-terminating 3′-terminal nucleoside inhibitors such as AZT forms the basis of HIV-1 resistance to such drugs, and this removal is enhanced by thymidine analog mutations (TAMs). We found that both wt and TAM-containing RTs were able to catalyze Pi-mediated phosphorolysis of 3′-terminal AZT at physiological levels of Pi with an efficacy similar to that for ATP-dependent AZT-excision. Conclusion: We have identified two new catalytic function of HIV-1 RT, the use of dNDPs as substrates for DNA synthesis, and the use of Pi as substrate for phosphorolytic removal of primer 3′-terminal nucleotides. The ability to insert dNDPs has been documented for only one other DNA polymerase The RB69 DNA polymerase and the reverse reaction employing inorganic phosphate has not been documented for any DNA polymerase. Importantly, our results show that Pi-mediated phosphorolysis can contribute to AZT resistance and indicates that factors that influence HIV resistance to AZT are more complex than previously appreciated. © 2008 Garforth et al

    Generation of Catalytic Films of Zeolite Y and ZSM-5 on FeCrAlloy Metal

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    This work details the generation of thin films of structured zeolite catalysts (ZSM–5 and Y) onto the surface of a metal substrate (FeCrAlloy) using in-situ hydrothermal synthesis. In addition, the zeolite Y is post-synthetically modified by acidified ammonium ion exchange to generate US-Y. Finally the catalytic activity of the structured ZSM-5 catalyst films (Si/Al = 11, thickness 146 µm) and structured US–Y catalyst film (Si/Al = 8, thickness 23µm) were compared with the pelleted powder form of ZSM–5 and USY catalysts of similar Si/Al ratios. The structured catalyst films have been characterised using a range of techniques, including X-ray diffraction (XRD), Electron microscopy (SEM), Energy Dispersive X–ray analysis (EDX) and Thermogravimetric Analysis (TGA). The transition from oxide-on-alloy wires to hydrothermally synthesised uniformly zeolite coated surfaces was followed using SEM and XRD. In addition, the robustness of the prepared coating was confirmed by subjecting these to thermal cycling (ambient to 550°C). The cracking of n–heptane over the pellets and structured catalysts for both ZSM–5 and Y zeolite showed very similar product selectivities for similar amounts of catalyst with an apparent activation energy of around 60 kJ mol-1. This paper demonstrates that structured catalysts can be manufactured with excellent zeolite adherence and when suitably activated/modified give comparable cracking results to the pelleted powder forms. These structured catalysts will improve temperature distribution in highly exothermic and endothermic catalysed processes
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