Influence of cold storage time on the softening prediction in Spring Bright nectarines

With Time-resolved Reflectance Spectroscopy (TRS) the maturity of nectarines at harvest can be assessed by measuring the absorption coefficient at 670 nm (µa 670) in the fruit flesh. A kinetic model has been developed linking the optical properties as measured by TRS with the models of µa 670 and firmness decay in shelf-life at 20°C, making the prediction of the softening time for individual fruit possible. In order to study the influence of cold storage time prior to shelf life on the softening prediction, 540 (year 2003) and 870 (year 2004) ‘Spring Bright’ nectarines were measured at harvest with TRS; then fruit were put in shelf life after various periods of cold storage at 0°C (4 and 10 d, year 2003; 6, 13 and 20 days, year 2004). During the 5-day period of shelf life at 20°C, fruit were analysed for firmness by pressure test after 30, 48, 54, 72, 78, 96, 102 and 120h in 2003 and after 36, 43, 62, 87, 108 and 135h in 2004. For each year and cold storage time, the parameters of the logistic model of softening as a function of µa 670 at harvest were computed. The cold storage up to 13 days did not significantly influence the estimates of the softening rate constant (kf), of the maximum firmness at minus infinite time (Fmax) and of parameter alpha (a) in both years, whereas parameter beta (ß) in 2003 significantly decreased from -1.867 at day 4 to -2.237 at day 10. The further 7 days of cold storage in 2004 significantly affected kf, which decreased from 0.00084 at days 6 and 13 to 0.00069 at day 20, and ß which increased from -2.395 at day 6 to -2.053 at day 20. Our results indicate that the cold storage time significantly influences the softening prediction of nectarines as the longer the cold storage, the lower the softening rat

Survey of Italian pediatricians on awareness, experiences and beliefs regarding direct-to-consumer genetic testing in minors

Background: Our study wanted to assess Italian pediatricians’ awareness, experience and beliefs regarding directto- consumer (DTC) genetic tests (GT) in minors, with a focus on those for predisposition to complex disease, lyfestyle, athletic ability and other inborn talents. Methods: A 28-item questionnaire was administered through the SurveyMonkey® web platform to the 9,086 members of the Italian Society of Pediatrics for which a valid email address was available. The survey was opened from April through November 2017. Statistical analyses were performed using the Graphpad software package. Results: 36.2% of the 442 respondents were aware of DTC-GT, but only 23.1% of them felt adequately prepared to meet families’ information needs. The first three sources of knowledge were the Internet (20.98%), magazines/ newspapers (16.78%) and TV/Radio (14.33%), while companies’ direct marketing activity influenced knowledge only in 2.45% of the cases. Only 16.4% of the aware respondents had been already approached for advice. More than 95% of the pediatricians who were aware would not advise DTC-GT for lifestyle, athletic performance or other inborn skills. 69.2% was unfavourable to susceptibility tests for complex diseases. Most of them expressed an interest in learning more and indicated as preferred sources of information public policies issued by professional societies. Conclusion: The low awareness and experience and the vendors’ tiny contribution to knowledge suggest a still limited penetration of DTC-GT companies in Italy. A great interest in learning more was found. Scientific societies are best positioned to support health professionals in this educational goal thanks to their role of trusted sources of information and guidance

Feeding behaviour of larval European sea bass (Dicentrarchus labrax L.) in relation to temperature and prey density

The feeding behaviour of larval European sea bass (Dicentrarchus labrax, L.) was analysed in relation to temperature and prey density under controlled laboratory conditions with the aim to assess the ability of larval fish to change the feeding tactic as a response to environmental changes. Larvae were acclimated for 20 days at three different temperatures (19, 22 and 26°C), and their feeding behaviour was then video-recorded in experimental trials, at two prey densities, consisting of swarms of 400/l and 1440/l Artemia nauplii. Results showed that there was a significant effect of the interaction between temperature and prey density on the proportion of swimming activity that was reduced at the high temperature-high prey density combination. This suggested a switching in the larval feeding behaviour from an active to an ambush tactic, when the temperature reached 26°C and the prey density was 1440 /l Artemia nauplii. These results are consistent with the current literature on fish larval behaviour in showing that the foraging tactic can be modulated by the interaction of different abiotic and biotic factors characterising the rearing environment

Sensory profiles of various stored fruit species are affected by maturity class assessed by time-resolved reflectance spectroscopy at harvest

The absorption coefficient measured at harvest at 670 nm (μa670) by timeresolved reflectance spectroscopy (TRS) is a non-destructive maturity index used to evaluate the biological age of fruit, i.e., the fruit maturity stage. The μa670 was successfully used to classify nectarine, apple and mango fruit into maturity classes each one having distinctive ripening behaviors. Aiming at studying the influence of TRS maturity class assessed at harvest on the sensory profiles of various fruit species after storage, 'Jonagored' apples, 'Abbé Fétel' pears, 'Morsiani 90' nectarines and 'Spring Belle' peaches were measured at harvest by TRS at 670 nm, ranked on the basis of decreasing μa670 (increasing maturity) and classified as less (LeM), medium and more (MoM) mature. Then fruit were randomized into batches of 30 fruits, each one corresponding to a storage atmosphere (apples: CA, NA; pears: CA, NA, DCA) or to a storage temperature (peaches, nectarines: 0, 4°C). After storage, fruit were put in shelf life at 20°C to reach the ripening degree for consumption. Sensory analyses (QDA profiles) were carried out on LeM and MoM fruit using a panel of 10 assessors. Our results indicate that, for all the species, besides the influence of storage conditions, there was also a great influence of TRS maturity classification at harvest. MoM apples and pears developed physiological disorders such as mealiness and graininess when stored in NA, whereas showed well balanced sensory characteristics when stored in CA and DCA, becoming after storage soft, juicy, sweet, but sour enough. MoM nectarines stored both at 0 and 4°C became woolly, while the LeM ones stored at 0°C developed the best sensory characteristics. MoM peaches stored at 0°C showed the best sensory profile and the LeM ones stored at 4°C the worst. So TRS maturity classification at harvest can give indications on the best storage conditions in order to obtain fruit with sensory characteristics than can satisfy the consumer

TRS-measurements as a nondestructive method assessing stage of maturity and ripening in plum (Prunus domestica L.)

n plum fruit with dark red or blue blush colour covering the whole fruit, the change in ground colour from green to yellow during maturation and ripening is masked. Hence, the maturity stage is difficult to judge. Time-resolved reflectance spectroscopy (TRS) has been used as a nondestructive method to assess changes in important internal quality factors in ‘Jubileum’ plums (Prunus domestica L.). Absorption coefficients (µa) and scattering coefficients (µs) were measured at both 670 and 758 nm during 5 days of storage. The changes in soluble solids content, titratable acidity and firmness were as expected. No change in soluble solids content was observed, while the plums became less acid and softer during storage. The TRS-measurements of plums indicated that TRS could give interesting information on internal quality factors in plums as the absorption at 670 nm was closely related to firmness, TA and TSS at the time of picking. Absorption at 758 nm was more closely related to the quality parameters after storage. The study did not indicate that scattering could be used in assessing maturity stage in plum

Optical Absorption and Scattering Phenomena in 'Jubileum' Plums in Relation to Their Colour Properties

Absorption and scattering of laser light pulse passing through the fruit determine among others, the optical properties of the product. Efforts have been made in the recent past to utilize innovative techniques such as time-resolved reflectance spectroscopy (TRS) to study the quality aspects of different fruit such as nectarines. These optical properties have been well related to firmness, sugars, acids and other quality attributes. TRS measurements were performed on ‘Jubileum’ plums at two different wavelengths: 670 nm and 758 nm. The fruit were harvested in Norway and brought to Italy under protected conditions. After sorting the fruit by size, TRS measurements were made and the fruit were randomized for different examinations of quality aspects. It was observed that the absorption coefficient (µa) increased for both wavelengths as ripening progressed towards the melting stage of the fruit. The µa values at 670 nm were higher than those at 758 nm. The higher rate in the µa was distinguishable from the third day onwards as the fruit ripened. Similarly, it was interesting to note that the internal colour measured after destructing the fruit related well with the TRS absorption coefficient (µa), i.e., a decrease in the CIE L* (towards darker region) and b* (towards blue) value along with an increase in a* (towards red) from third day of storag

Assessing the Harvest Maturity of Brazilian Mangoes

No clear criterion exists to determine the optimum time to harvest mango. Some empirical relations are used to assess maturity, such as shoulder development. Moreover, as a result of the typical growing conditions in tropical climates, a huge variation in maturity and ripeness exists, seriously hampering the export of fruit in the global chain. The consequence for consumers in western countries is that sometimes mangoes are overripe at the retailer, or have to be kept for several days, even weeks, to reach the edible state, provided they do not rot in the meantime. To ensure an edible quality, the chlorophyll content in the fruit flesh, measured at harvest by Time-resolved Reflectance Spectroscopy (TRS), could be used as a maturity criterion for mango fruit. Commercially grown fruit were harvested in Brazil and transported to Italy by plane. Fruits were measured using TRS at 630 nm for absorption coefficient (µa) and skin colour. The development of µa was followed on 60 fruits during 15 days of storage at 20°C. The remainders of fruit were used to measure firmness destructively. Absorption coefficient decreased during shelf life according to a logistic pattern, as expected for colour development. Taking the variation between the individual fruit into account, 72% of the variation was accounted for. Nevertheless, µa assessed at harvest could be converted into a biological shift factor (BSF), as an expression of the maturity at harvest of each individual fruit. This biological shift factor explained about 70% of the variation in firmness development in individual fruit. These preliminary results indicate that TRS methodology coupled with BSF theory could be useful in assessing maturity at harvest and assuring acceptable eating quality of mango

Linking food web functioning and habitat diversity for an ecosystem based management: A Mediterranean lagoon case-study

We propose a modelling approach relating the functioning of a transitional ecosystem with the spatial extension of its habitats. A test case is presented for the lagoon of Venice, discussing the results in the context of the application of current EU directives. The effects on food web functioning due to changes related to manageable and unmanageable drivers were investigated. The modelling procedure involved the use of steady-state food web models and network analysis, respectively applied to estimate the fluxes of energy associated with trophic interactions, and to compute indices of food web functioning. On the long term (hundred years) temporal scale, the model indicated that the expected loss of salt marshes will produce further changes at the system level, with a lagoon showing a decrease in the energy processing efficiency. On the short term scale, simulation results indicated that fishery management accompanied by seagrass restoration measures would produce a slight transition towards a more healthy system, with higher energy cycling, and maintaining a good balance between processing efficiency and resilience. Scenarios presented suggest that the effectiveness of short term management strategies can be better evaluated when contextualized in the long term trends of evolution of a system. We also remark the need for further studying the relationship between habitat diversity and indicators of food web functioning