Characterization of Electrochemically Deposited Ce1-xZrxO2 Layers Modified with Cobalt Oxide for Electrocatalytic Conversion of NOx and CO

A method for electrochemical deposition of a two-component Ce1−xZrxO2 system on stainless steel substrate that is attractive from catalytic point of view is proposed. As reported in the literature, it is a promising carrier layer for the production of catalytic converters for purification of exhaust gases containing NOx and CO. This system is modified by electrodeposition of a thin film of cobalt oxide over it. A series of samples of the Ce1−xZrxO2/СоxОy system was produced with various concentrations and proportions of the components. These samples are characterized by XRD, SEM, EDS, XPS and РCС (partial electrocatalytic curves) of CO oxidation and of NОx reduction. Based on the obtained results, it has been concluded that the electrodeposited two-component Ce1−xZrxO2 system is a solid solution with composition, structure, and physicochemical properties that make it suitable for use as active phase carrier for catalytic oxidation of CO and reduction of NOx

Insights into volcanic hazards and plume chemistry from multi-parameter observations: the eruptions of Fimmvörðuháls and Eyjafjallajökull (2010) and Holuhraun (2014–2015)

The eruptions of Eyjafjallajökull volcano in 2010 (including its initial effusive phase at Fimmvörðuháls and its later explosive phase from the central volcano) and Bárðarbunga volcano in 2014–2015 (at Holuhraun) were widely reported. Here, we report on complementary, interdisciplinary observations made of the eruptive gases and lavas that shed light on the processes and atmospheric impacts of the eruptions, and afford an intercomparison of contrasting eruptive styles and hazards. We find that (i) consistent with other authors, there are substantial differences in the gas composition between the eruptions; namely that the deeper stored Eyjafjallajökull magmas led to greater enrichment in Cl relative to S; (ii) lava field SO2 degassing was measured to be 5–20% of the total emissions during Holuhraun, and the lava emissions were enriched in Cl at both fissure eruptions—particularly Fimmvörðuháls; and (iii) BrO is produced in Icelandic plumes in spite of the low UV levels

Effects of a recombinant gene expression on ColE1-like plasmid segregation in Escherichia coli

<p>Abstract</p> <p>Background</p> <p>Segregation of expression plasmids leads to loss of recombinant DNA from transformed bacterial cells due to the irregular distribution of plasmids between the daughter cells during cell division. Under non-selective conditions this segregational instability results in a heterogeneous population of cells, where the non-productive plasmid-free cells overgrow the plasmid-bearing cells thus decreasing the yield of recombinant protein. Amongst the factors affecting segregational plasmid instability are: the plasmid design, plasmid copy-number, host cell genotype, fermentation conditions etc. This study aims to investigate the influence of transcription and translation on the segregation of recombinant plasmids designed for constitutive gene expression in <it>Escherichia coli </it>LE392 at glucose-limited continuous cultivation. To this end a series of pBR322-based plasmids carrying a synthetic human interferon-gamma (hIFNγ) gene placed under the control of different regulatory elements (promoter and ribosome-binding sites) were used as a model.</p> <p>Results</p> <p>Bacterial growth and product formation kinetics of transformed <it>E. coli </it>LE392 cells cultivated continuously were described by a structured kinetic model proposed by Lee et al. (1985). The obtained results demonstrated that both transcription and translation efficiency strongly affected plasmid segregation. The segregation of plasmid having a deleted promoter did not exceed 5% after 190 h of cultivation. The observed high plasmid stability was not related with an increase in the plasmid copy-number. A reverse correlation between the yield of recombinant protein (as modulated by using different ribosome binding sites) and segregational plasmid stability (determined by the above model) was also observed.</p> <p>Conclusions</p> <p>Switching-off transcription of the hIFNγ gene has a stabilising effect on ColE1-like plasmids against segregation, which is not associated with an increase in the plasmid copy-number. The increased constitutive gene expression has a negative effect on segregational plasmid stability. A kinetic model proposed by Lee et al. (1985) was appropriate for description of <it>E. coli </it>cell growth and recombinant product formation in chemostat cultivations.</p

Stem cells in liver regeneration and therapy

The liver has adapted to the inflow of ingested toxins by the evolutionary development of unique regenerative properties and responds to injury or tissue loss by the rapid division of mature cells. Proliferation of the parenchymal cells, i.e. hepatocytes and epithelial cells of the bile duct, is regulated by numerous cytokine/growth-factor-mediated pathways and is synchronised with extracellular matrix degradation and restoration of the vasculature. Resident hepatic stem/progenitor cells have also been identified in small numbers in normal liver and implicated in liver tissue repair. Their putative role in the physiology, pathophysiology and therapy of the liver, however, is not yet precisely known. Hepatic stem/progenitor cells also known as “oval cells” in rodents have been implicated in liver tissue repair, at a time when the capacity for hepatocyte and bile duct replication is exhausted or experimentally inhibited (facultative stem/progenitor cell pool). Although much more has to be learned about the role of stem/progenitor cells in the physiology and pathophysiology of the liver, experimental analysis of the therapeutic value of these cells has been initiated. Transplantation of hepatic stem/progenitor cells or in vivo pharmacological activation of the pool of hepatic stem cells may provide novel modalities for the therapy of liver diseases. In addition, extrahepatic stem cells (e.g. bone marrow cells) are being investigated for their contribution to liver regeneration. Hepatic progenitor cells derived from embryonic stem cells are included in this review, which also discusses future perspectives of stem cell-based therapies for liver diseases