Fluidization-mediated tissue spreading by mitotic cell rounding and non-canonical Wnt signalling

Tissue morphogenesis is driven by mechanical forces that elicit changes in cell size, shape and motion. The extent by which forces deform tissues critically depends on the rheological properties of the recipient tissue. Yet, whether and how dynamic changes in tissue rheology affect tissue morphogenesis and how they are regulated within the developing organism remain unclear. Here, we show that blastoderm spreading at the onset of zebrafish morphogenesis relies on a rapid, pronounced and spatially patterned tissue fluidization. Blastoderm fluidization is temporally controlled by mitotic cell rounding-dependent cell–cell contact disassembly during the last rounds of cell cleavages. Moreover, fluidization is spatially restricted to the central blastoderm by local activation of non-canonical Wnt signalling within the blastoderm margin, increasing cell cohesion and thereby counteracting the effect of mitotic rounding on contact disassembly. Overall, our results identify a fluidity transition mediated by loss of cell cohesion as a critical regulator of embryo morphogenesis

Different strategies for midline formation in bilaterians

The evolutionary emergence of the bilateral body plan and the central nervous system required the establishment of a midline organizer. The formation of a solitary, elongated but narrow organizing region for the dorsoventral (or mediolateral) axis requires rather complex molecular interactions. Different modes of midline formation evolved in vertebrates, insects and planarians, indicating that midline formation had a crucial role in the diversification of higher organisms