Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days

The aim of the present study was to investigate the influence of the protein content of seminal plasma on the motility, viability and acrosome integrity of spermatozoa in extended semen stored for 3 days. A total of 32 semen samples (from four boars) with high (4 mg/ml) and 32 semen samples (from four boars) with low (2 mg/ml) protein content were investigated. The semen samples were diluted by BTS at a ratio of 1:4, and stored for 72 h at 17oC. The percentages of live sperm (LS), live sperm with damaged acrosome (LDA) and total sperm with damaged acrosome (TDA) were detected by flow cytometry. Sperm progressive motility (PM) was detected using CASA. After 72 h of storage, the percentage of LS and PM was significantly (P lt 0.01) higher, and the LDA and TDA were significantly (P lt 0.01) lower in samples with high protein content than in the samples with low protein content (LS = 66 vs. 44%, PM = 64 vs. 48%, LDA = 15 vs. 21% and TDA = 29 vs. 45%, respectively). When comparing the difference between 0 and 72 h of storage, the percentage decrease in LS and PM, while increase in LDA and TDA were significantly higher in the samples with low (LS: 75 to 44%; PM: 68 to 48%; LDA: 11 to 21% and TDA: 23 to 45%) than in the samples with high protein content (LS: 78 to 66%; PM: 70 to 64%; LDA: 9 to 15% and TDA: 17 to 29%). We concluded that protein content in seminal plasma has a significant influence on progressive motility, viability and acrosome integrity in diluted semen stored for 3 days

Influence of transcervical infusion of seminal plasma on the farrowing rate and litter size in artificially inseminated sows

ABSTRACT Recent studies have focused on the use of seminal plasma to increase sow fertility after classical intracervical artificial insemination (AI). The aim of the present study was to investigate the influence of seminal plasma infusion, prior to the application of conventional AI dose, on the fertility rate in sows. A total of 114 sows were treated with intrauterine infusion of 30ml seminal plasma (SP), while 114 control sows were infused by physiological solution (PS), immediately before the application of conventional AI dose. The experiment was conducted at one commercial pig farm in Serbia, which is comprised of 1,500 sows in the breeding herd. Intrauterine infusion of seminal plasma produced significantly (P&lt;0.05) higher farrowing rate (93.8%) and significantly (P&lt;0.01) more live-born piglets per litter (12.27), compared with the control sows (83.33% farrowing rate and 10.48 piglets). The present results show that intrauterine infusion of seminal plasma can be a useful tool for increasing the fertility rate in artificially inseminated sows, under the conditions of practical intensive pig production.</jats:p

Proteingehalt im Samenplasma von Zuchtebern auf den Betrieben für intensive Schweineproduktion in Serbien

The aim of this study was to investigate: (a) seminal plasma protein content in the Land-race (n = 38), Large White (n = 44) and Duroc (n = 24) boars breed, as well as boars distribution according to protein content in seminal plasma, and (b) the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17°C for 3 days. Not significant (p > 0.05) differences was found in seminal plasma protein content between three boar breeds. Only 31% of the 106 investigated boars had > 3.6% protein in seminal plasma. Progressive motility was significantly higher (p lt 0.01) in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%). After 3 days of storage, in thel:4 dilution proportion, the average progressive motility was significantly (p lt 0.01) decreased in relation to this value in native semen from the boars with high (82% to 64%), as well from the boars with low protein content in seminal plasma (76% to 48%). However, the average diluted semen progressive motility was significantly greater (p lt 0.01) in the boars with high (64%), compared to the boars with low protein content in seminal plasma (48%). The number of good diluted semen samples (>65% progressive motility), was also significantly (p lt 0.01) greater in the boars with high (41%), compared to the boars with low protein content in seminal plasma (12%)

Breeding and exploitation of boars on commercial farm

Breeding and exploitation of boars on commercial farms aimed at producing sperm doses for personal use. The lifetime of boars and therefore the length of their exploitation largely depend on their health. Therefore, control of the health status of the boars, quality control sperm for artificial insemination by overseeing the entire process of taking to sperm quality insemination dose optimization and comprehensive environmental conditions hold (accommodation, microclimate, food, power, attitude of employees), represent important parameters in terms of health control themselves boars, health control of the entire herd or economic parameters and productivity of farms and the profitability of the entire production. On a commercial farm at a total of 28 boar, race Landrace and Jokshir who were in exploitation during one calendar year following parameters: the number of inseminated gilts, sows number insemintion , repeated heats, the number of pollinated piglets, number of live births, and the number of stillborn piglets. Of all the 28 boars that were in exploitation were took blood. Sera boars we used to study prervalence porcine circovirus antibodies to type 2 (PCV2) using the ELISA test. We found that out of 28 tested samples was positive 21 samples. We have established the prevalence of antibodies to porcine circovirus type 2 PCV2 in tested boars