Differential Expression Profile and Genetic Variants of MicroRNAs Sequences in Breast Cancer Patients

The technology available for cancer diagnosis and prognosis is not yet satisfactory at the molecular level, and requires further improvements. Micro RNAs (miRNAs) have been recently reported as useful biomarkers in diseases including cancer. We performed a miRNA expression profiling study using peripheral blood from breast cancer patients to detect and identify characteristic patterns. A total of 100 breast cancer patients and 89 healthy patients were recruited for miRNA genotyping and expression profiling. We found that hs-miR-196a2 in premenopausal patients, and hs-miR-499, hs-miR-146a and hs-miR-196a2 in postmenopausal patients, may discriminate breast cancer patients from healthy individuals. In addition, we found a significant association between two microRNA polymorphisms (hs-miR-196a2 and hs-miR-499) and breast cancer risk. However, no significant association between the hs-miR-146a gene and breast cancer risk was found. In summary, the study demonstrates that peripheral blood miRNAs and their expression and genotypic profiles can be developed as biomarkers for early diagnosis and prognosis of breast cancer

Characterising paediatric mortality during and after acute illness in Sub-Saharan Africa and South Asia: a secondary analysis of the CHAIN cohort using a machine learning approach

Background A better understanding of which children are likely to die during acute illness will help clinicians and policy makers target resources at the most vulnerable children. We used machine learning to characterise mortality in the 30-days following admission and the 180-days after discharge from nine hospitals in low and middle-income countries (LMIC). Methods A cohort of 3101 children aged 2–24 months were recruited at admission to hospital for any acute illness in Bangladesh (Dhaka and Matlab Hospitals), Pakistan (Civil Hospital Karachi), Kenya (Kilifi, Mbagathi, and Migori Hospitals), Uganda (Mulago Hospital), Malawi (Queen Elizabeth Central Hospital), and Burkina Faso (Banfora Hospital) from November 2016 to January 2019. To record mortality, children were observed during their hospitalisation and for 180 days post-discharge. Extreme gradient boosted models of death within 30 days of admission and mortality in the 180 days following discharge were built. Clusters of mortality sharing similar characteristics were identified from the models using Shapley additive values with spectral clustering. Findings Anthropometric and laboratory parameters were the most influential predictors of both 30-day and post-discharge mortality. No WHO/IMCI syndromes were among the 25 most influential mortality predictors of mortality. For 30-day mortality, two lower-risk clusters (N = 1915, 61%) included children with higher-than-average anthropometry (1% died, 95% CI: 0–2), and children without signs of severe illness (3% died, 95% CI: 2–4%). The two highest risk 30-day mortality clusters (N = 118, 4%) were characterised by high urea and creatinine (70% died, 95% CI: 62–82%); and nutritional oedema with low platelets and reduced consciousness (97% died, 95% CI: 92–100%). For post-discharge mortality risk, two low-risk clusters (N = 1753, 61%) were defined by higher-than-average anthropometry (0% died, 95% CI: 0–1%), and gastroenteritis with lower-than-average anthropometry and without major laboratory abnormalities (0% died, 95% CI: 0–1%). Two highest risk post-discharge clusters (N = 267, 9%) included children leaving against medical advice (30% died, 95% CI: 25–37%), and severely-low anthropometry with signs of illness at discharge (46% died, 95% CI: 34–62%). Interpretation WHO clinical syndromes are not sufficient at predicting risk. Integrating basic laboratory features such as urea, creatinine, red blood cell, lymphocyte and platelet counts into guidelines may strengthen efforts to identify high-risk children during paediatric hospitalisations. Funding Bill & Melinda Gates Foundation OPP1131320

Tuberculosis - sarcoidosis association: a case report

Keywords: tuberculous sarcoidosis; caseating granuloma; non-caseating granulomaDOI: http://dx.doi.org/10.3329/jcmcta.v22i1.9111 JCMCTA 2011; 22(1): 35-38</jats:p

Pneumatosis Cystoides Intestinalis: A rare cause of intestinal obstruction

There are many well-known causes of acute or sub-acute intestinal obstruction. But Pneumatosis cystoides intestinalis (PCI) is a rare cause and can easily be misdiagnosed. PCI refers to presence of air within the wall of small or large intestine. This benign disease having both idiopathic and secondary causes with different contributing pathogenic factors that confuses many doctors. In most of the time it is asymptomatic, but clinical signs and imaging impressions may mimic true abdominal visceral perforation or soft tissue stranding leads to mechanical obstruction. PCI is sometimes incidentally found during radiological evaluation, endoscopic procedure or laparotomy. Surgery is not recommended for PCI if there was no intestinal obstruction, because of high operative risk. So correct diagnosis is imperative as treatment of PCI is generally conservative. We are presenting a case of intestinal obstruction who underwent to laparotomy followed by the histopathological diagnosis as a case of PCI.  KYAMC Journal Vol. 13, No. 03, October 2022: 177-180  </jats:p

Prevalence of Human Rift Valley Fever Virus as a Bio-threat in Asian Countries after COVID-19 Lockdown

Abstract not available&#x0D; Bangladesh Journal of Infectious Diseases, October 2020;7(suppl_2):S72-S74</jats:p

Sociodemographic Determinants of Personality Disorder In Patients of Obsessive-compulsive Disorder

Background &amp; objective: Obsessive Compulsive Disorder (OCD) is the 4th most prevalent psychiatric disorder in Epidemiological Catchment Area (ECA) Survey and most individuals with OCD have co-morbid personality disorders (PD). The present study was undertaken to find the prevalence of PD among patients with OCD and the sociodemographic determinants that influence the development PD in patients with OCD.&#x0D; Methods:The cross-sectional analytical study was conducted in OCD clinic of Bangabandhu Sheikh Mujib Medical University from January 2015 to September 2016. Respondents were recruited from the OCD patients diagnosed on the basis of DSM-IV criteria and face to face interview was conducted with the help of the Structured Clinical Interview for DSM-IV Axis II Disorders (SCID-II questionnaire). The main outcome variable was PD in patients of OCD and the exposure variables (determinants) were selected sociodemographic characteristics.&#x0D; Result: The mean age of the OCD patients was (28 ± 8.7) years. Almost half (49%) of OCD patients belonged to the age group 20-30 years. Female respondents were 53.6%. Among the respondents, 62% had co-morbid PD. The mean age of the OCD patients with PD was observed to be significantly higher (29.1 ± 9.1 years) than those without PD (26.2 ± 7.8 years) (p = 0.047). Male OCD patients were 2.4 (95% CI of OR: 1.2 – 4.7) times more likely to have PD than their female counterparts (p = 0.012). Ethnic groups other than Muslims tend to be associated with PD more often and carry more than 8-fold (95% CI of OR: 8.4 – 37.2) higher risk of having the condition (p = 0.001). Likewise, other occupants were nearly 2(95% CI of OR: 0.98 – 3.74) times more prone to have PD than the students (p = 0.056).&#x0D; Conclusion: Personality Disorders are highly prevalent among people with OCD which remains underdiagnosed &amp; under-addressed. Male OCD patients, non-Muslims and occupants other than students are more prone to have PD. Persons with Personality Disorders are far more likely to refuse psychiatric help and deny their problems.&#x0D; Ibrahim Card Med J 2017; 7 (1&amp;2): 44-50 </jats:p

A Comparative Study between Endoscopic Septoplasty and Conventional Septoplasty

Background: Conventional nasal septal surgery depending on head light improves the nasal airway but leaves some complications, but with recent advancement in endoscopic approach limits the complication and improves the outcome. This is due to better visualization, improved illumination, avoiding unnecessary manipulation, limited exposure and scope for second surgery if required.&#x0D; Aim and objective: To assess the functional outcome of endoscopic septoplasty over conventional septoplasty.&#x0D; Material and method: This study was carried out in the department of Otolaryngology – Head &amp; Neck Surgery of Bangladesh Medical College Hospital and Popular Medical College Specialized Hospital over a period of 5 years (2014- 2019). Ethical clearance was obtained from Hospital authority. All consecutive patients with symptomatic deviated nasal septum who were refractory to medical treatment were included in the study. Patients were equally divided into two groups of conventional and endoscopic septoplasty. Patients &amp; their attendants, or legal guardians (in patients of less than 18 years) were thoroughly briefed about the each operative procedure. Patients with acute rhinitis or allergic rhinitis or vasomotor rhinitis or upper respiratory tract infection, external deformity, patients unfit for surgeries and patients not giving consent were excluded from the study. After surgery all patients were followed up monthly, 3 monthly and 6 monthly for 01 year. At each follow up visit, subjective and objective assessments were done. Objective assessment was done by Gertner-Podoshin plate and diagnostic nasal endoscopy.&#x0D; Results: In this study, nasal obstruction was relieved in 91% of conventional and 95% in endoscopic septoplasty group. Headache was improved in 75.68% and 86.5% patients and snoring was improved in 79.5% &amp; 88.6% of patients of respectively. Intraoperative complications such as hemorrhage (21.67% vs 3.33%) and mucosal tear (28.33% vs 5%) are significantly higher in conventional septoplasty group than the endoscopy group.&#x0D; Conclusion: The use of endoscope in septal surgery improves visualization, illumination, and thus reduces complication with improved functional outcome. It can be performed in other diseases of nose and para nasal sinus diseases in the perspective of functional endoscopic sinus surgery.&#x0D; J Bangladesh Coll Phys Surg 2021; 39(3): 178-184</jats:p

Advantages and Limitations of Endoscopic Septoplasty: Experience of 120 Cases

Objective: Traditional surgeries for the deviated nasal septum improves the nasal airway but recent development and advancement of the knowledge about endoscope has changed the treatment modality significantly. Endoscopic approach under good Illumination and magnification lessen the complication. This study is done to see the advantages and limitations of endoscopic septoplasty Method: Total 120 cases of symptomatic deviated nasal septum, refractory to conservative medical treatment were randomly selected on the basis of clinical examination in a single institute. It was a prospective study, performed to see the advantages and limitations of endoscopic septoplasty. Informed written consent was taken in all cases. All the patients underwent endoscopic septoplasty under general anesthesia after proper evaluation. Results: There was a slight male predominance. 13 year child was the youngest patient and 58 was the oldest. During endoscopic septoplasty a large number of patients needed some additional surgery like, inferior turbinoplasty in 27 (22.5%), functional endoscopic sinus surgery in 11 patients (9.16%) etc. The complication rate was minimum (4.16%). Patient’s satisfaction was high. Though there were some limitations but we observed so many advantages of this procedure. Conclusion: Though conventional Septoplasty is widely practiced by most ENT surgeons till now, the endoscopic approach can be considered as a better alternative</jats:p