Coherent and Incoherent Dynamics in Quantum Dots and Nanophotonic Devices

The interest in coherent and incoherent dynamics in novel semiconductor gain media and nanophotonic devices is driven by the wish to understand the optical gain spectrally, dynamically, and energetically for applications in optical amplifiers, lasers or specially designed multi-section devices. This chapter is devoted to the investigation of carrier dynamics inside nanostructured gain media as well as to the dynamics of the resulting light output. It is structured into two parts. The first part deals with the characterization of ultrafast and complex carrier dynamics via the optical response of the gain medium with pump-probe methods, two-color four-wave mixing setups and quantum-state tomography. We discuss the optical nonlinearities resulting from light-matter coupling and charge carrier interactions using microscopically motivated rate-equation models. In the second part, nanostructured mode-locked lasers are investigated, with a focus on analytic insights about the regularity of the pulsed light emission. A method for efficiently predicting the timing fluctuations is presented and used to optimize the device properties. Finally, one specific design of a mode-locked laser with tapered gain section is discussed which draws the attention to alternative ways of producing very stable and high intensity laser pulses

Membrane Insertion for the Detection of Lipopolysaccharides: Exploring the Dynamics of Amphiphile-in-Lipid Assays

<div><p>Shiga toxin-producing <i>Escherichia coli</i> is an important cause of foodborne illness, with cases attributable to beef, fresh produce and other sources. Many serotypes of the pathogen cause disease, and differentiating one serotype from another requires specific identification of the O antigen located on the lipopolysaccharide (LPS) molecule. The amphiphilic structure of LPS poses a challenge when using classical detection methods, which do not take into account its lipoglycan biochemistry. Typically, detection of LPS requires heat or chemical treatment of samples and relies on bioactivity assays for the conserved lipid A portion of the molecule. Our goal was to develop assays to facilitate the direct and discriminative detection of the entire LPS molecule and its O antigen in complex matrices using minimal sample processing. To perform serogroup identification of LPS, we used a method called membrane insertion on a waveguide biosensor, and tested three serogroups of LPS. The membrane insertion technique allows for the hydrophobic association of LPS with a lipid bilayer, where the exposed O antigen can be targeted for specific detection. Samples of beef lysate were spiked with LPS to perform O antigen specific detection of LPS from <i>E</i>. <i>coli</i> O157. To validate assay performance, we evaluated the biophysical interactions of LPS with lipid bilayers both in- and outside of a flow cell using fluorescence microscopy and fluorescently doped lipids. Our results indicate that membrane insertion allows for the qualitative and reliable identification of amphiphilic LPS in complex samples like beef homogenates. We also demonstrated that LPS-induced hole formation does not occur under the conditions of the membrane insertion assays. Together, these findings describe for the first time the serogroup-specific detection of amphiphilic LPS in complex samples using a membrane insertion assay, and highlight the importance of LPS molecular conformations in detection architectures.</p></div