Effect of chitosan on propagation of zamiifolia as tropical ornamental indoor plant by leaf cutting

Zamiifolia (Zamioculcas zamiifolia) is an ornamental and perennial medicinal plant, which belongs to Araceae family. This plant holds a prominent place in the economic significance of this particular plant family. This study aimed to examine the process of root development and rhizome production through leaf cuttings of zamiifoliain the this experiment. This experiment was conducted as factorial design within a completely randomized framework design with three replications. Experimental treatments composed of chitosan application at three levels (0, 250 and 500 mg L-1), and the positioning of leaflet cuttings along the main axis of the mother leaf (apical, middle, and basal). After the application of the treatments, the leaflet cuttings were subjected to a four-month rooting period. This rooting process took place in a growth medium consisting of a balanced mixture of perlite and cocopeat mixed in a volume ratio of 1:1. The results exhibited significant effects of different chitosan concentrations on several parameters, including rhizome number, rhizome width, the number of roots, and the quality of the mother leaf. In addition, the type of leaflet cutting demonstrated a significant influence on the width and number of rhizomes. These results demonstrated that the application of chitosan at concentrations of 250 and 500 mg L-1 had a positive effect and resulted in increased rhizome number, rhizome width, and number of roots. Overall, it can be concluded that chitosan can promote the growth and development of zamiifolia by stimulating rhizome production and improving root proliferation

Things Are Getting Hairy: Enterobacteria Bacteriophage vB_PcaM_CBB

Enterobacteria phage vB_PcaM_CBB is a jumbo phage belonging to the family Myoviridae. It possesses highly atypical whisker-like structures along the length of its contractile tail. It has a broad host range with the capability of infecting species of the genera Erwinia, Pectobacterium, and Cronobacter. With a genome of 355,922 bp, excluding a predicted terminal repeat of 22,456 bp, phage CBB is the third largest phage sequenced to date. Its genome was predicted to encode 554 ORFs with 33 tRNAs. Based on prediction and proteome analysis of the virions, 29% of its predicted ORFs could be functionally assigned. Protein comparison shows that CBB shares between 3338% of its proteins with Cronobacter phage GAP32, coliphages PBECO4 and 121Q as well as Klebsiella phage vB_KleM_Rak2. This work presents a detailed and comparative analysis of vB_PcaM_CBB of a highly atypical jumbo myoviridae phage, contributing to a better understanding of phage diversity and biology.Funding was provided by Cork Institute of Technology as a PhD fellowship to CB

Complete genome sequence of bacteriophage vB_YenP_AP5 which infects Yersinia enterocoliticaof serotype O:3

BACKGROUND: Bacteriophage vB_YenP_AP5 is a lytic bacteriophage capable of infecting Yersinia enterocolitica strains of serotype O:3, an epidemiologically significant serotype within this bacterial species that causes yersiniosis in humans. This work describes the complete genome sequence of this phage. RESULTS: The genome consists of linear double-stranded DNA of 38,646 bp, with direct terminal repeats of 235 bp in length, and a GC content of 50.7%. There are 45 open reading frames which occupy 89.9% of the genome. Most of the proteins encoded by this virus exhibit sequence similarity to Yersinia phage φYeO3-12 and Salmonella phage φSG-JL2 proteins. CONCLUSIONS: Genomic and morphological analyses place the bacteriophage vB_YenP_AP5 in the T7likevirus genus of the subfamily Autographivirinae within the family Podoviridae

Assessing phage therapy against Pseudomonas aeruginosa using a Galleria mellonella infection model

The Galleria mellonella infection model was used to assess the in vivo efficacy of phage therapy against laboratory and clinical strains of Pseudomonas aeruginosa. In a first series of experiments, Galleria were infected with the laboratory strain P. aeruginosa PAO1 and were treated with varying multiplicity of infection (MOI) of phages either 2 h post-infection (treatment) or 2 h pre-infection (prevention) via injection into the haemolymph. To address the kinetics of infection, larvae were bled over a period of 24 h for quantification of bacteria and phages. Survival rates at 24 h when infected with 10 cells/larvae were greater in the prevention versus treatment model (47% vs. 40%, MOI = 10; 47% vs. 20%, MOI = 1; and 33% vs. 7%, MOI = 0.1). This pattern held true when 100 cells/larvae were used (87% vs. 20%, MOI = 10; 53% vs. 13%, MOI = 1; 67% vs. 7%, MOI = 0.1). By 24 h post-infection, phages kept bacterial cell numbers in the haemolymph 1000-fold lower than in the non-treated group. In a second series of experiments using clinical strains to further validate the prevention model, phages protected Galleria when infected with both a bacteraemia (0% vs. 85%) and a cystic fibrosis (80% vs. 100%) isolate. Therefore, this study validates the use of G. mellonella as a simple, robust and cost-effective model for initial in vivo examination of P. aeruginosa-targeted phage therapy, which may be applied to other pathogens with similarly low infective doses

Figure S5: vConTACT family of tools on CyVerse

Taxonomic classification of archaeal and bacterial viruses is challenging, yet also fundamental for developing a predictive understanding of microbial ecosystems. Recent identification of hundreds of thousands of new viral genomes and genome fragments, whose hosts remain unknown, requires a paradigm shift away from traditional classification approaches and towards the use of genomes for taxonomy. Here we revisited the use of genomes and their protein content as a means for developing a viral taxonomy for bacterial and archaeal viruses. A network-based analytic was evaluated and benchmarked against authority-accepted taxonomic assignments and found to be largely concordant. Exceptions were manually examined and found to represent areas of viral genome ‘sequence space’ that are under-sampled or prone to excessive genetic exchange. While both cases are poorly resolved by genome-based taxonomic approaches, the former will improve as viral sequence space is better sampled and the latter are uncommon. Finally, given the largely robust taxonomic capabilities of this approach, we sought to enable researchers to easily and systematically classify new viruses. Thus, we established a tool, vConTACT, as an app at iVirus, where it operates as a fast, highly scalable, user-friendly app within the free and powerful CyVerse cyberinfrastructure

Biocontrol of Cronobacter spp. using Bacteriophage in Infant Formula

The purpose of this research was to explore the potential application of lytic phages to control Cronobacter spp. in infant formula. More than two hundred and fifty phages were isolated from various environmental samples against different strains of Cronobacter spp. Selected phages were characterized by morphology, host range, and cross infectivity. The genomes of five novel Cronobacter phages [vB_CsaM_GAP31 (GAP31), vB_CsaM_GAP32 (GAP32), vB_CsaP_GAP52 (GAP52), vB_CsaM_GAP161 (GAP161), vB_CsaP_GAP227 (GAP227)] were sequenced. Phage GAP32 possess the second largest phage genome sequenced to date, and it is proposed that GAP32 belongs to a new genus of “Gap32likeviruses”. Phages GAP52 and GAP227 are the first C. sakazakii podoviruses whose genomes have been sequenced. None of the sequenced genomes showed homology to virulent or lysogenic genes. In addition, in vivo administration of phage GAP161 in the hemolymph of Galleria mellonella larvae showed no negative effects on the wellbeing of the larvae and could effectively prevent Cronobacter infection in the larvae. A cocktail of five phages was highly effective for biocontrol of three Cronobacter sakazakii strains present as a mixed culture in both broth media and contaminated reconstituted infant formula. This phage cocktail could be potentially used to control C. sakazakii during preparation of infant formula but would first have to be clinically evaluated in mammalian models.Natural Sciences and Engineering Research Council of CanadaDairy Farmers of Ontari

Preliminary Evaluation of the Impact of Antioxidants Polyvinylpyrrolidone and Ascorbic Acid on Patch Budding of Persian Walnut

The purpose of this study is to investigate the effect of antioxidants on the budding success of three walnut genotypes under greenhouse conditions. After stratification, seeds were planted in a greenhouse at the end of autumn. After about 20 months, the seedlings grew up to 80 cm. The budding was done using scions of Z30, Z60, and B21 genotypes. Scions were immersed in polyvinylpyrrolidone (PVP) or ascorbic acid in the concentrations 2, 3, and 4 g·dm−3 for 1 h before budding. Percentage of successful budding was determined after one month. The results showed that genotype significantly affected the percentage of budding success, but no interaction between genotype and treatments was found. The antioxidant solutions significantly affected all the parameters studied in this research. Ascorbic acid with 3 g·dm−3 resulted in the highest percentage of budding success (86.66%); no significant differences were observed with 4 g·dm−3 of ascorbic acid and 3 and 4 g·dm−3 of PVP. Furthermore, antioxidant solutions significantly affected the content of chlorophylls, and 3 g·dm−3 of ascorbic acid resulted in the highest content of chlorophylls in scion leaves

Evaluation of the Relation between Water Pollution and Economic Growth in Developing Countries and Developed Countries

Most studies suggest a form of inverted U relationship between environmental quality and economic growth, entitled as "the environmental Kuzne's curve (EKC)" Given the importance of maintaining environmental quality along with economic growth, this study studies the level of water pollution and economic growth both in developing and developed countries during 2001-1980. Based on theoretical foundations of environmental EKC curve, as in addition to the income variable, the population variable, is also considered as an exogenous variable and the integration method is used for estimating the model. The results indicates that the economic conditions prevailing in both groups of countries are consistent with the EKC. Curve and it this curve. The turning point in developed countries is at 10,622 dollars based on statistical findings, most of the studied countries in this group have crossed the turning point. But as for developing countries, the turning point in this curve is estimated at approximately 901 dollars of national per capita income. Statistical analysis of existing data showed that many countries at this group still have not reached the turning point of the EKC curve and are far from it. Using the results, it seems necessary for the developing countries to adopt more cautious policies to grow and develop their economies, in order to promote the growth of national per capita income, as well as policies to improve quality of water resources, or at least prevent their contamination