Cytogenotoxicity biomarkers in fat snook Centropomus parallelus from Cananéia and São Vicente estuaries, SP, Brazil

The aquatic environment receives many contaminants that can induce damages at the molecular, biochemical, cellular and physiological levels. Centropomus parallelus, an important food resource for local populations, is a predator fish that feeds on small fishes and benthic invertebrates, thus being vulnerable to the bioconcentration and biomagnification processes. This study aimed to evaluate cytogenotoxic responses in erythrocytes from C. parallelus juveniles collected in the Cananéia and São Vicente estuaries, both in winter and in summer. After anesthesia, blood samples were collected by caudal puncture. Blood smears were prepared on glass slides and stained with May-Grünwald-Giemsa dye. Two thousand cells were analyzed per slide (1000x), and nuclear abnormalities (NA) and micronuclei (MN) were scored. The São Vicente sample showed MN and NA frequencies (%/1000 cells) of 0.325 and 3.575, in winter, and of 0.125 and 2.935 in summer respectively; the Cananéia sample showed frequencies of 0.0325 and 0.03, in winter, and of 0.065 and 0.355 in summer, respectively. The rates found in São Vicente were significantly higher than those found in Cananéia, evidencing that the levels of pollution in that estuary were high enough to induce genetic damages

Fluoxetine can make marine organisms unhappy: a study on the sub-lethal effects on marine invertebrates

The environmental effects caused by selective serotonin reuptake inhibitor drugs have been investigated for marine organisms and coastal ecosystems but are scarce in neotropical organisms. This investigation aimed to evaluate the sublethal effects of fluoxetine on the embryonic development of the sea urchin Echinometra lucunter and the survival and swimming behavior of the brine shrimp Artemia sp. The organisms were exposed to four different concentrations of fluoxetine (30, 300, 3000 and 30000 ng L-1) and to a negative control (filtered seawater), following the respective standard testing protocols. We verified a significant reduction of the embryos development to pluteus larvae, starting from 3000 ng L-1 (54.0±10.9% normal larvae), in comparison with the controls (83.5±3.1%). The non-observed effect concentration (NOEC) was estimated at 300 ng L-1, and the lowest observed effect concentration (LOEC) was 3000 ng L-1. In the behavior tests with Artemia sp, no significant adverse effects were reported for mobility, swimming speed and inactivity time. These results show that Fluoxetine can interfere on the development of species like the sea urchin E. lucunter, but short term exposure did not affected the swimming behavior of the brine shrimp Artemia sp. Fluoxetine presents thus a potential to affect marine biota and disrupt the equilibrium of the coastal ecosystems.</jats:p