Method Development for the Determination of the Amino Acid Profile of Algae via HPLC

Undergraduate Basi

Mathematica code for implementing the aggregate EMF

This report contains the Mathematica code, used to implement the aggregate EMF spectral density due to several nearby transmitters

Non-linear echo cancellation - a Bayesian approach

Echo cancellation literature is reviewed, then a Bayesian model is introduced and it is shown how how it can be used to model and fit nonlinear channels. An algorithm for cancellation of echo over a nonlinear channel is developed and tested. It is shown that this nonlinear algorithm converges for both linear and nonlinear channels and is superior to linear echo cancellation for canceling an echo through a nonlinear echo-path channel

HPLC Analysis of Amino Acids in Algae

Undergraduate Basi

An incubatable direct current stimulation system for in vitro studies of Mammalian cells.

The purpose of this study was to provide a simplified alternative technology and format for direct current stimulation of mammalian cells. An incubatable reusable stimulator was developed that effectively delivers a regulated current and does not require constant monitoring

Lipid content and biomass analysis in autotrophic and heterotrophic algal species

Biofuels are a form of renewable energy derived from living matter, typically plants. The push for biofuels began in order to decrease the amount of carbon dioxide (CO2) released into the atmosphere, as biofuels are essentially carbon neutral. The idea is the same amount of CO2 the plants took in to perform photosynthesis will then be released in the burning of the biofuels. Algae is an excellent source of biofuels because it grows quickly and is versatile in terms of the type of fuel it can produce. The two most common mechanisms for algae growth are heterotrophic or photoautotrophic. Heterotrophically grown algae uses an exogenous energy source, such as glucose, and uses the energy stored in it to perform cellular functions. Glucose also serves as a source of carbon and hydrogen, which are the primary elements found in lipids. In addition heterotrophic algae requires other nutrients for survival, such as water, vitamins, and inorganic ions. Algae grown photoautotrophically uses pigments in cellular photoreceptors to convert energy from light into adenosine triphosphate (ATP), an energy source, and to produce glucose. It also requires water, vitamins, and inorganic ions like the heterotrophic algae does. Some algal species, such as Chlorella zofingiensis, can be grown both photoautotrophically and heterotrophically. This algae species will be the subject of our experiment. Our experiment seeks to discover the most efficient way of growing algae to produce the highest amount of lipids. In addition to serving as a key component of cell and organelle membranes, lipids are a common form of high efficiency, long-term energy storage for living organisms, which is why lipids are extracted and processed to form biofuels. We propose growing one species of algae photoautotrophically by providing it with proper amounts of light but eliminating any glucose available. We will also grow the same species heterotrophically, with exogenous access to glucose, but eliminating all exposure to light sources. Finally, we will grow the same species mixotrophically with access to both glucose and light. Once the algae is grown, it will be harvested and analyzed for its lipid profile to determine which algae sample has the highest percent lipid content. We will also measure the percent biomass of each sample to determine which primary energy source leads to the greatest amount of total algal growth, percent organic material, and percent lipid content. We predict the algae grown with access to both sunlight and exogenous glucose will produce both the highest lipid content and the highest percent of biomass

Engaging Universities as Partners and Proponents of the New Urban Agenda: Coordination, Spatial Strategies, Access

Research Brief for UN-Habitat UNI and the UN-Habitat Working Group on Higher Education for Sustainability

‘It’s Not Going to be Suburban, It’s Going to be All Urban’: Assembling Post-suburbia in the Toronto and Chicago Regions

Urban and suburban politics are increasingly intertwined in regions that aspire to be global. Powerful actors in the Chicago and Toronto regions have mobilized regional space to brand rescaled images of the urban experience, but questions remain as to who constructs and who can access the benefits of these revised spatial identities. Local political interests have tended to be obfuscated in the regional milieu, most problematically in the spaces between the gentrified inner cities, privileged growth nodes, and the glamorized suburban subdivisions and exurban spaces beyond the city limits. This article analyses how socio-spatial changes in post-suburbanizing urban fringes contribute to the way regions are being reconfigured and reimagined. Guided by current debates at the intersection of assemblage theory and critical urban political economy, our analysis demonstrates how socio-technical infrastructures, policy mobilities and political economic relations are spatially aligned, sustained and dissolved in splintering North American agglomerations. Particular attention is paid to issues of urban transportation and connectivity in uncovering multifaceted modes of suburbanism that now underlie the monistic imagery of the globalized region. Emergent regionalized topologies and territoriality blur conventional understandings of city–suburban dichotomies in extended urban areas that are now characterized by polycentric post-suburban constellations. In terms of their substance and functionality, ‘real existing’ regions are currently re-territorialized as complex assemblages that are embedded in a neoliberalizing political economy whose politics and identities are only beginning to be revealed