Mycosporine-like Amino Acids and Other Phytochemicals Directly Detected by High-Resolution NMR on Klamath (Aphanizomenon flos-aquae) Blue-Green Algae

This study describes for the first time the use of high-resolution nuclear magnetic resonance (NMR) on Klamath (Aphanizomenon flos-aquae, AFA) blue-green algae directly on powder suspension. These algae are considered to be a "superfood", due to their complete nutritional profile that has proved to have important therapeutic effects. The main advantage of NMR spectroscopy is that it permits the detection of a number of metabolites all at once. The Klamath alga metabolome was revealed to be quite complex, and the most peculiar phytochemicals that can be detected directly on algae by NMR are mycosporine-like amino acids (porphyra-334, P334; shinorine, Shi) and low molecular weight glycosides (glyceryl \u3b2-d-galactopyranoside, GalpG; glyceryl 6-amino-6-deoxy-\u3b1-d-glucopyranoside, ADG), all compounds with a high nutraceutical value. The presence of cis-3,4-DhLys was revealed for the first time. This molecule could be involved in the anticancer properties ascribed to AFA

\u3a0\u2011Stacking Signature in NMR Solution Spectra of Thiophene-Based Conjugated Polymers

Studies on conjugated polymers seldom report on their NMR characterization in solution. This paper shows how NMR experiments, both 1H NMR and routine 2D NMR spectra, can help in gaining a further insight into the aggregation behavior of conjugated polymers and could be used to flank the more employed solid-state NMR and other spectroscopy and microscopy techniques in the understanding of the aggregation processes. NMR spectroscopy allows distinguishing, within the class of poorly solvatochromic conjugated polymers, those highly prone to form \u3c0-stacked aggregates from the ones that have a low tendency toward \u3c0-stacking

Tissue metabolomics and HR-MAS NMR

High-Resolution Magic Angle Spinning (HR-MAS) Nuclear Magnetic Resonance (NMR) can play an important role among the analytical methods that can be used in metabolomics. It was developed more than 25 years ago,[1] and since then it has been used to analyze cells and biological tissues in many fields: biochemical, biomedical,[2] plants,[3] agriculture,[4] food,[5] etc. Typical problems tackled are information on cellular metabolisms of physiological and pathological processes, response to some kind of stress, differences among cultivars or varieties, metabolism in little living organisms. HR-MAS NMR has become an important platform for metabolomic studies. The technique allows the detection of the metabolic profile directly on intact tissue samples and cells, producing NMR spectra with resolution that can be comparable to that obtained from sample extract solutions, avoiding complex metabolite extraction processes, and preserving tissue and cellular structures. These studies can be based on the analysis of metabolic profiles in their entirety or on the quantification of individual metabolites or metabolite ratios. The basis of the technique, some critical issues and some examples of application in different contexts will be shown. [1] L. L. Cheng, C. L. Lean, A. Bogdanova, S. C. Wright, Jr, J. L. Ackerman, T. J. Brady, L. Garrido, Magnetic resonance in medicine (1996), 36, 653-8 [2] L. L Cheng, NMR in biomedicine (2023), 36, e4684. [3] C. Deborde, A. Moing, L. Roch, D. Jacob, D. Rolin, P. Giraudeau, Progress in Nuclear Magnetic Resonance Spectroscopy (2017), 102-103, 61-97. [4] P. Mazzei, A. Piccolo, Chemical and Biological Technologies in Agriculture (2017), 4, 11/1-11/13. [5] C. Corsaro, N. Cicero, D. Mallamace, S. Vasi, C. Naccari, A. Salvo, S. V. Giofre, G. Dugo, Food Research International (2016), 89(Part_3), 1085-1094

AFM phase imaging of soft-hydrated samples: A versatile tool to complete the chemical-physical study of liposomes

Despite of the several approaches applied to the physicochemical characterization of liposomes, few techniques are really useful to obtain information about the surface properties of these colloidal drug-delivery systems. In this paper, we demonstrate a possible new application of tapping mode atomic force microscopy (AFM) to discriminate between conventional and pegylated liposomes. We showed that the differences on liposomal surface properties revealed by the phase images AFM approach well correlate with the data obtained using classical methods, such as light scattering, hydrodynamic, and nuclear magnetic resonance analysis

MRS study of meningeal hemangiopericytoma and edema: A comparison with meningothelial meningioma

Intracranial hemangiopericytomas (HPCs) are rare tumors and their radiological appearance resembles that of meningiomas, especially meningothelial meningiomas. To increase the knowledge on the biochemical composition of this type of tumor for better diagnosis and prognosis, we performed a molecular study using ex vivo high resolution magic angle spinning (HR-MAS) magnetic resonance spectroscopy (MRS) perfomed on HPC and peritumoral edematous tissues. Moreover, to help in the discrimination between HPC and meningothelial meningioma we compared the ex vivo HR-MAS spectra of samples from one patient with HPC and 5 patients affected by meningothelial meningioma. Magnetic resonance imaging (MRI), in vivo localized single voxel 1H-MRS was also performed on the same patients prior to surgery and the in vivo and ex vivo MRS spectra were compared. We observed the presence of OH-butyrate, together with glucose in HPC and a low amount of N-acetylaspartate in the edema, that may reflect neuronal alteration responsible for associated epilepsy. Many differences between HPC and meningothelial meningioma were identified. The relative ratios of myo-inositol, glucose and gluthatione with respect to glutamate are higher in HPC compared to meningioma; whereas the relative ratios of creatine, glutamine, alanine, glycine and choline-containing compounds with respect to glutamate are lower in HPC compared to meningioma. These data will be useful to improve the interpretation of in vivo MRS spectra resulting in a more accurate diagnosis of these rare tumors

Molecular characterization of human gastric mucosa by HR-MAS Magnetic Resonance Spectroscopy

The present study was aimed at identifying themolecular profile characteristic of the healthy humangastric mucosa.Ex vivo HR-MAS magnetic resonance spectroscopy performed at 9.4 Tesla (400.13 MHz for 1H) on gastric specimens collected during endoscopy, permits the identification of more than forty species giving a detailed picture of the biochemical pattern of the gastric tissues. These preliminary data will be used for a comparison with gastric preneoplastic and neoplastic situations. Moreover, the full knowledge of the biochemical pattern of the healthy gastrictissues is the necessary presupposition for the application of magnetic resonance spectroscopy directly in vivo

Alcune applicazioni dell'NMR in stato solido

comunicazione durante il Workshop Risonanza Magnetica Nucleare tenutosi l'8 novembre 2024 dal titolo Alcune applicazioni dell'NMR in stato solid

Ex vivo HR-MAS Magnetic Resonance Spectroscopy of human gastric adenocarcinomas: A comparison with healthy gastric mucosa

The present study reports the characteristics of the biochemical profile of human gastric adenocarcinoma in comparison with that of healthy gastric mucosa, using ex vivo HR-MAS Magnetic Resonance Spectroscopy. Healthy human mucosa is mainly characterized by the presence of small metabolites (more than 50 identified) and macromolecules, whereas the adenocarcinoma spectra are dominated by the presence of signals due to triglycerides, whose content on the contrary is very low in healthy gastric mucosa. The use of spin-echo experiments enable us to detect some metabolites in the unhealthy tissues and to determine their variation with respect to the healthy ones. We have observed that the Cho:ChoCC ratio changes from 20:80 in the healthy tissues to 80:20 in the neoplastic gastric mucosa

STUDY OF THE EFFECTS OF SHORT-CHAIN PFASs ON HUMAN CELLS WITH NMR-METABOLOMICS

Application of NMR-based Metabolomics to study the effects of shortchain PFASs on an in vitro mode