Opioid mediated activity and expression of mu and delta opioid receptors in isolated human term non-laboring myometrium

The existence of opioid receptors in mammalian myometrial tissue is now widely accepted. Previously enkephalin degrading enzymes have been shown to be elevated in pregnant rat uterus and a met-enkephalin analogue has been shown to alter spontaneous contractility of rat myometrium. Here we have undertaken studies to determine the effects of met-enkephalin on in vitro human myometrial contractility and investigate the expression of opioid receptors in pregnant myometrium. Myometrial biopsies were taken from women undergoing elective caesarean delivery at term. Organ bath experiments were used to investigate the effect of the met-enkephalin analogue [d-Ala 2, d-met 5] enkephalin (DAMEA) on spontaneous contractility. A confocal immunofluorescent technique and real time PCR were used to determine the expression of protein and mRNA, respectively for two opioid receptor subtypes, mu and delta. DAMEA had a concentration dependent inhibitory effect on contractile activity (1×10−7 M–1×10−4 M; 54% reduction in contractile activity, P<0.001 at 1×10−4 M concentration). Mu and delta opioid receptor protein sub-types and their respective mRNA were identified in all tissues sampled. This is the first report of opioid receptor expression and of an opioid mediated uterorelaxant action in term human non-labouring myometrium in vitroSB. 21/03/201

Protective effects of selenium against potassium dichromate-induced hematotoxicity in female and male Wistar albino rats

Objective: Potassium dichromate (K2Cr2O7) is a potent pollutant for human and animal health. The purpose of the current work is to compare the effect of K2Cr2O7 using variations in the dose, route of administration and duration of exposure in male and female Wistar albino rats and to research the interaction of chromium and selenium with a special focus on hematopoiesis. Materials and methods: K2Cr2O7 was subcutaneously administered alone (10, 50 and 100 mg/kg body weight) or K2Cr2O7 (10 mg/kg) in association with selenium (0.3 mg/kg) was administered to female Wistar albino rats. Male rats received in their drinking water K2Cr2O7 (30 mg/L/day) alone or in association with Se (0.3 mg/L/day) for 20 consecutive days. The hematological parameters were evaluated on days 3, 6 and 21 after subcutaneous (sc.) treatment in female rats and on days 10 and 20 after oral administration in male rats. Results: K2Cr2O7− induced during the first three days a significant (p < 0.05) dose-dependent decrease in the number of erythrocytes, platelets, leucocytes, lymphocytes and the hematocrit levels, and a dose-dependent increase in the number of granulocytes and monocytes. In the drinking water, chromium sc. significantly decreased the number of leucocytes and lymphocytes on day 10 after treatment and elevated the number of granulocytes and monocytes 20 days later. Selenium sc. counterbalanced the hematotoxic effects of chromium in female rats. Conclusion: These results suggest that the selenium has a protective role against the hematotoxicity of subcutaneous chromium in female Wistar rats

الأغنية الشعبية النسوية بمنطقة الغزوات- جمع ودراسة

هذا البحث جهد متواضع يحوي في طياته فكرة صغيرة عن الأغنية الشعبية النسوية في منطقة الغزوات . إننا نحاول التعمق في الأعماق، أعماق الجماهير الشعبية،نبحث عن الجذور ،عن الجوهر، و عن روح المرأة المحافظة على ثراثها الشفاهي الأصيل ، نستجليها من أغاني شعبية تحمل موضوعات تمسّ جانبا كبيرا من أحاسيسها و وجدانها . أودّ أن أضع هذا البحث بين يدي القارىء ليتفحصها و إني لسعيدة بذلك

Proposition d’un processus de prévision d'une entreprise industrielle Un cas d'étude : « LIT MAG »

Ce travail concerne une étude de planification des approvisionnements appliqué à l’entreprise LIT MAG en trouvant la méthode adaptable au nôtre série chronologique des données historique liées aux demandes clientes, nous aurons utilisé deux logiciels de calculs statistiques Eviews pour la méthode Boc Jenkins et l’Excel pours les autres méthodes pour obtenir des valeurs bien précis en effectuent plusieurs tests. L’objectif de cette étude est d’avoir un processus de prévision développé pour une bonne gestion d’approvisionnement au sein de l’entreprise LIT MAG. Pour le premier chapitre nous avons définir et expliquer les notions de base, le deuxième chapitre est une présentation d’entreprise LIT MAG et dans le troisième chapitre nous avons effectué notre application numérique sur nos données des années précédentes

ETUDE TECHNICO-ECONOMIQUE D’UN BATIMENT EN BETON ARME "S/SOL ET RDC + 10 ETAGES"

Ce projet consiste principalement en l'étude dynamique est économique d'une structure (R+10+S/Sol) contreventée par voiles a usage multiple( habitation,commerce). La structure est implantée a Oran zone de moyenne sismicité selon règlement parasismique algériennes 99 modifies en 2003. L'analyse dynamique de cette structure a été faite par logiciel de calcul SAP2000

The Blood Biochemical Profiles in Algerian Rabbits of the Semi-arid Region of Aures (Algeria) At Different Physiological Stages

Background: In recent years, several works have examined the reproductive traits of Algerian local population rabbit does but no standardization study has been done on the plasmatic parameters of this population of rabbits reared in the Aures region during their different physiological stages. Therefore, the present study evaluated the effect of these on certain blood parameters in local rabbit does.Methods: The rabbit does (n=120) were randomly divided into 4 treatment groups; Empty (E), Pregnant (P), Pregnant lactating (P-L) and Lactating (L). Does were first mated at the age of 16.5 weeks with an average body weight of 3107.1 ± 53.5g and with body condition scoring (BCS) of loin and rump ranging from 2 to 3 on a scale of 1- 5 following a 42-day rhythm. Four blood samples were collected from females of five rabbits in each group viz. E, P, P-L and L respectively.Result: Milk yield differs significantly (p less than 0.05) between the two experimental groups (P-L and L). Litter size and litter weight of does at birthing was higher (p less than 0.01) in L group than in P and P-L groups. That the mean levels glucose in non-pregnant group recorded as 0.22±0.04g/l which was increased 3rd week of pregnancy (0.65± 0.1 g/l followed by low mean value 0.35+0.2g/l and 0.4+0.1g/l at pregnant lactating and lactating stages of experimental rabbits. The lowest level of protein was recorded in the second week of pregnancy (p less than 0.05). The increase in total protein, albumin and globulin concentrations in lactating group might be physiological. Concerning total lipids, there was a strong mobilization of body fat in pregnant females (97 mg/dl ± 0.10; p less than 0.05). However, a marked decrease (p less than 0.05) was recorded in cholesterolemia in lactating females. Regarding triglyceridemia, we have recorded a significant raise (37.7 ± 35.6; p less than 0.001) in pregnant females. The creatinine value recorded was close to the physiological norms 1.3-2.7 mg/dl vs 1.08 -0. 92 mg/dl. T3 plasma levels were lower (p less than 0.05) in lactating females’ group. Pregnant and lactating does showed an increase (p less than 0.05) of leptin level. The higher (p less than 0.05) progesterone levels in pregnant and pregnant-lactating does than its concentrations in empty and lactating does might be attributed to maintaining pregnancy. Therefore, the current study confirms that the analysis of metabolites represents a good tool to understand the physiological mechanisms. </jats:p