Enlargement and Integration Action Activity Workshop on Costs, Benefits and Impact Assessment of Smart Grids for Europe and Beyond

Smart Grids are a key component of the European strategy toward a low-carbon energy future. Growing environmental and energy security concerns represent a major driver for the renovation and improvement of existing energy infrastructure. In this context, Enlargement and Integration countries will have to face substantial investments in the coming years to upgrade and modernise their energy networks towards smart power grids. Wind and solar electricity retain the greatest potential to contribute and increase the shares of renewable electricity production; however, current electricity transmission and distribution systems do not generally appear adequate to reliably cope with large-scale penetration of such variable renewables based generating plants (whether centralised or distributed). Significant investments will need to be mobilized. Most energy investments are long life and capital intensive, therefore investment decisions taken now will have an impact for many years. When planning the electricity system of the future, it is necessary to adopt an integrated approach to assess the interrelated physical, environmental, cyber, social, economic and policy challenges where a fair allocation of short term costs and long term benefits among different players is a precondition for reducing uncertainties and incentivize investments. In this context the workshop will discuss how these developments can provide examples and opportunities for E&I countries to build smart grids and will present and discuss approaches and methodologies for cost – benefit analysis that should include all the costs and benefits that smart grid projects can bring to the energy system at large and to society. The workshop will discuss the impacts of smart grids not only in monetary terms, but also through the identification of externalities and social impacts that can result from the implementation of Smart Grid. The workshop will benefit from the on-going experience in Enlargement and Integration Countries on smart grid developments.JRC.F.3-Energy Security, Systems and Marke

The Roles of Individual Mammalian Argonautes in RNA Interference In Vivo

Argonaute 2 (Ago2) is the only mammalian Ago protein capable of mRNA cleavage. It has been reported that the activity of the short interfering RNA targeting coding sequence (CDS), but not 3′ untranslated region (3′UTR) of an mRNA, is solely dependent on Ago2 in vitro. These studies utilized extremely high doses of siRNAs and overexpressed Ago proteins, as well as were directed at various highly expressed reporter transgenes. Here we report the effect of Ago2 in vivo on targeted knockdown of several endogenous genes by siRNAs, targeting both CDS and 3′UTR. We show that siRNAs targeting CDS lose their activity in the absence of Ago2, whereas both Ago1 and Ago3 proteins contribute to residual 3′UTR-targeted siRNA-mediated knockdown observed in the absence of Ago2 in mouse liver. Our results provide mechanistic insight into two components mediating RNAi under physiological conditions: mRNA cleavage dependent and independent. In addition our results contribute a novel consideration for designing most efficacious siRNA molecules with the preference given to 3′UTR targeting as to harness the activity of several Ago proteins.Alnylam Pharmaceuticals (Firm

Poly(amidoamine)s synthesis, characterisation and interaction with BSA

Cationic poly(amidoamine)s (PAAs) were synthesised and characterised by NMR and gel permeation chromatography. Their thermal properties were investigated using thermogravimetric analysis and differential scanning calorimetry. Although poly(amidoamine)s have been used as endosomolytic polymers for protein intracellular delivery, the interaction of the polymers with the proteins still need to be investigated. BSA was used as a model protein and complexation with the different poly(amidoamine) s was investigated using gel retardation assays, fluorescence spectroscopy and high sensitivity differential scanning calorimetry. Our results indicate that the thermal stability of BSA was affected upon interaction and complexation with the poly(amidoamine)s, however these interactions did not seem to modify the structure of the protein. Polymer flexibility seemed to favour polymer/protein complexation and promoted thermal stability

Environmental Impacts of Emergency Livestock Mortality Composting—Leachate Release and Soil Contamination

A 3-year study was conducted in Iowa to evaluate the feasibility of using composting for emergency disposal of cattle mortalities. During the study, 49 metric tons of 450 kg cattle carcasses were composted in 27 replicated unturned windrow test units constructed during three different seasons of the year. Each test unit contained 1.8 metric tons of carcasses enveloped in one of 5 different materials: corn silage, ground cornstalks, straw/manure, leaves, or a soil/compost blend. Due to their water absorbing capacity and ability to evaporate absorbed water, the volume of leachate released into the soil was generally less than 5% of the 500-600 mm of precipitation that fell on the test units. Chemical analysis of 1.2 m deep soil cores collected from beneath the composting test units prior to and following composting showed statistically significant increases in chloride concentrations at all depths beneath composting test units constructed from silage, cornstalks, straw, and the soil/compost blend. Statistically significant increases in % total carbon (silage test units only) and % total nitrogen (silage, cornstalk, straw/manure test units) were limited to the top 15 cm of soil. Increases in these pollutants were moderate, amounting to less than 5X, 0.2X and 0.4 X respectively of chloride, % total carbon, % total N concentrations prior to composting. Statistically significant increases in total ammonia-nitrogen were noted at depths of up to 90 cm beneath test units constructed with silage or leaves, and at 30 cm and 15 cm depths respectively beneath test units constructed with straw/manure and cornstalks. The ammonia-nitrogen increases were large, ranging from 40-160 X of pre-composting levels of ammonia in the topsoil. When compared with the groundwater pollution potential of carcass burial, however, the estimated total mass of N contained in the composted cattle carcasses was 4-10 X the increases in total N measured in the soil beneath the composting test units

Ionizable Amphiphilic Dendrimer-Based Nanomaterials with Alkyl-Chain-Substituted Amines for Tunable siRNA Delivery to the Liver Endothelium In Vivo

A library of dendrimers was synthesized and optimized for targeted small interfering RNA (siRNA) delivery to different cell subpopulations within the liver. Using a combinatorial approach, a library of these nanoparticle-forming materials was produced wherein the free amines on multigenerational poly(amido amine) and poly(propylenimine) dendrimers were substituted with alkyl chains of increasing length, and evaluated for their ability to deliver siRNA to liver cell subpopulations. Interestingly, two lead delivery materials could be formulated in a manner to alter their tissue tropism within the liver—with formulations from the same material capable of preferentially delivering siRNA to 1) endothelial cells, 2) endothelial cells and hepatocytes, or 3) endothelial cells, hepatocytes, and tumor cells in vivo. The ability to broaden or narrow the cellular destination of siRNA within the liver may provide a useful tool to address a range of liver diseases.National Institutes of Health (U.S.) Centers of Cancer and Nanotechnology Excellence (Grant U54 CA151884)Armed Forces Institute of Regenerative Medicine (Grant W81XWH-08-2-0034)Alnylam Pharmaceuticals (Firm

Role of hydration layer on rheology of nano alumina suspensions

Technological implication of reduction in viscosity of nanosize ceramic suspensions with environmentally benign and inexpensive additives is not trivial. This presentation will discuss the flow characteristics of concentrated nano-alumina powder suspensions. Unusually high viscosities observed for suspensions of nanoparticles compared to those of micron size powders cannot be explained by current viscosity models. For a given solids content, as the particle size decreases so does the interparticle distance leading to overlapping interparticle forces. Concomitant with the particle size reduction, increase in surface area of the solids requires higher surfactant concentrations for effective steric stabilization. The rheology of nanosize alumina suspensions and its variation with solids content and with saccharide concentration were explored by rheometry. The mechanism of dramatic viscosity reduction by saccharide addition (primarily fructose) is studied by TGA, DSC, and NMR. The interparticle forces between the nanometric alumina particles in water and in fructose solutions were investigated by AFM. The interactions between the nano-alumina particles in water can be explained by the DLVO theory. However, DLVO theory can not adequately describe the interactions between particles for suspensions containing saccharide. The interaction forces (amplitude and range) between nanometric alumina particles decrease with increasing saccharide concentration. Formation of so-called hydration layer on alumina nanoparticles in water was hypothesized for years, but never observed experimentally. The direct visualization of hydration layer over nanosize alumina particles was realized with the fluid cell transmission electron microscopy in situ. The hydration layer over the particle aggregates was observed and it was shown that these hydrated aggregates constitute new particle assemblies which in turn alter the flow behavior of the suspensions. These nanoclusters alter the effective solids content and the viscosity of nanosize alumina suspensions. Our findings elucidate the source of high viscosity observed for nano particle suspensions and are of direct relevance to many industrial sectors including materials, food, cosmetics, pharmaceutical among others employing colloidal slurries with nanosize particles

Cationic poly(amidoamine) promotes cytosolic delivery of bovine RNase A in melanoma cells, while maintaining its cellular toxicity

Ribonucleases are known to cleave ribonucleic acids, inducing cell death. RNase A, a member of the ribonuclease family, generally displayed poor in vitro activity. This has been attributed to factors such as low intracellular delivery. Poly(amidoamine)s have been used to promote the translocation of non-permeant proteins to the cytosol. Our objective was to demonstrate that poly(amidoamine)s could potentially promote the delivery of RNase A to selected cell line. Interactions of three cationic poly(amidoamine)s (P1, P2 and ISA1) with wild-type bovine RNase A were investigated using gel retardation assays, DLS and microcalorimetry. Although the polymers and the protein are essentially cationic at physiological pH, complexation between the PAAs and RNase A was observed. The high sensitivity differential scanning calorimetry (HSDSC) thermograms demonstrated that the thermal stability of the protein was reduced when complexed with ISA1 (Tmax decreased by 6.5 °C) but was not affected by P1 and P2. All the polymers displayed low cytotoxicity towards non-cancerous cells (IC50 > 3.5 mg mL?1). While RNase A alone was not toxic to mouse melanoma cells (B16F1), P1 was able to promote cytosolic delivery of biologically active RNase A, increasing cell death (IC50 = 0.09 mg mL?1)

Environmental Impact and Biosecurity of Composting for Emergency Disposal of Livestock Mortalities

A two-year project was begun in Iowa in 2002 to test the decay performance, air and water environmental impacts, and bio-security of using composting for emergency disposal of cattle carcasses in the event of a foot-and-mouth disease outbreak. Of the three emergency cover materials tested to date, silage produced the highest and most sustained internal heat, the most rapid and thorough carcass decomposition, and the most rapid destruction of avian vaccine viruses introduced into the piles for bio-security testing. Although internal temperatures within ground cornstalk cover material were much lower than in the silage, carcass decomposition appeared to occur almost as rapidly as in the silage. The cornstalk material produced more collectable leachate with higher pollutant concentrations than the silage. Downwind odor from test units constructed with ground cornstalk, which has a much higher air permeability coefficient than silage, appeared to be strongest and more frequent during the initial 2–3 weeks following construction of the piles