Clinical Usefulness of Cytokines as Diagnostic and Follow Up Markers in Patients with Stable Angina Pectoris

Background and aims: Angina pectoris is the discomfort felt when the heart muscle does not receive enough oxygen. The definition of stable angina is the presence of symptoms only with exertion. Chest pain, or its angina equivillant, is a defining feature, and it is relieved by rest or nitroglycerin when exercise is ceased. Often, this is one of the first symptoms or warning indications of underlying coronary disease. The proinflammatory state is believed to aggravate endothelial dysfunction by activating endothelial cells, releases cytokines and chemokines, which may be crucial in the atherosclerotic plaque formation which is the primary cause of angina pectoris. We studied the value of some cytokines and cardiac markers as a diagnostic and follow up markers in patients with stable angina. Methodology:  The study was carried out on 50 patients diagnosed with stable angina pectoris, who visited Nasiriya Heart Center throughout the period from December 2021 to April 2022,  and 20 age matched healthy person.  IL-1β, IL-5, IL-6, IL-8 were assayed at baseline (hospital admission), 1 day and 1 week post-PCI  by an enzyme-linked immunosorbent assay (ELISA). In addition,  Troponin I (cTnl) was determined by using monoclonal cTnI-specific antibody.&nbsp

The Analgesic and Anti-inflammatory Effect of Lawsone Isolated From Lawsonia Inermis

 This study aimed to assess the analgesic and anti-inflammatory effects of lawsone isolated from henna leaves (Lawsonia inermis).  Methods: A total of 120 healthy adult mice (weighing about 25±5 g) were included in this study. Sixty mice out of the total were used to determine LD50, 30 mice to determine the anti-inflammatory test, and the remaining (30 mice) were used for an analgesic test. The hot plate was used to determine the analgesic effect, while the anti-inflammatory effect was determined by the ability of the compound to minimize the inflammation and edema caused by the injection of carrageenan.  Results: Lawsone was isolated from Lawsonia inermis. A Stuart SMP10 digital melting point apparatus was used for measuring all melting points. Infrared spectrometer FT-IR 400D was used for measuring/recording IR spectra (KBr) which the frequency of absorption was represented as cm−1. For 1H-NMR spectrum recording, a Bruk-spectrophotometer of 400 MHz was used with internal TMS standard, with deuterated Ś 2.51 ppm for acetone-d6, remained solvent signals as well as 13C.NMR was used. TLC was utilized as adsorbent, UV light, or iodine-completed visualization to verify compounds' purities. The LD50 of the oral lawsone was 96 mg/kg, and the highest dosage that did not kill any of the experimental animals was 80 mg/kg, which was used to investigate lawsone's analgesic and anti-inflammatory effects. Lawsone and aspirin possessed an analgesic effect compared to the control group (p<0.0001 and p<0.001, respectively); however, lawsone induced a potent analgesic effect compared to aspirin (p<0.1). In contrast, Lawsone and aspirin exerted an anti-inflammatory effect (p<0.05) compared to the control group and were equipotent in carrageenan-induced hind paw edema. Conclusion: It is concluded that lawsone possesses analgesic and anti-inflammatory effects, which endorse the practical medical importance of Lawsonia inermis. The latter is widely used traditionally for these purposes own to its cost-effectiveness and safety; however, further studies are required to determine the systemic safety of lawson

The Analgesic and Anti-inflammatory Effect of Lawsone Isolated From Lawsonia Inermis

 This study aimed to assess the analgesic and anti-inflammatory effects of lawsone isolated from henna leaves (Lawsonia inermis).  Methods: A total of 120 healthy adult mice (weighing about 25±5 g) were included in this study. Sixty mice out of the total were used to determine LD50, 30 mice to determine the anti-inflammatory test, and the remaining (30 mice) were used for an analgesic test. The hot plate was used to determine the analgesic effect, while the anti-inflammatory effect was determined by the ability of the compound to minimize the inflammation and edema caused by the injection of carrageenan.  Results: Lawsone was isolated from Lawsonia inermis. A Stuart SMP10 digital melting point apparatus was used for measuring all melting points. Infrared spectrometer FT-IR 400D was used for measuring/recording IR spectra (KBr) which the frequency of absorption was represented as cm−1. For 1H-NMR spectrum recording, a Bruk-spectrophotometer of 400 MHz was used with internal TMS standard, with deuterated Ś 2.51 ppm for acetone-d6, remained solvent signals as well as 13C.NMR was used. TLC was utilized as adsorbent, UV light, or iodine-completed visualization to verify compounds' purities. The LD50 of the oral lawsone was 96 mg/kg, and the highest dosage that did not kill any of the experimental animals was 80 mg/kg, which was used to investigate lawsone's analgesic and anti-inflammatory effects. Lawsone and aspirin possessed an analgesic effect compared to the control group (p<0.0001 and p<0.001, respectively); however, lawsone induced a potent analgesic effect compared to aspirin (p<0.1). In contrast, Lawsone and aspirin exerted an anti-inflammatory effect (p<0.05) compared to the control group and were equipotent in carrageenan-induced hind paw edema. Conclusion: It is concluded that lawsone possesses analgesic and anti-inflammatory effects, which endorse the practical medical importance of Lawsonia inermis. The latter is widely used traditionally for these purposes own to its cost-effectiveness and safety; however, further studies are required to determine the systemic safety of lawson

Clinical Usefulness of Cytokines as Diagnostic and Follow Up Markers in Patients with Stable Angina Pectoris

Background and aims: Angina pectoris is the discomfort felt when the heart muscle does not receive enough oxygen. The definition of stable angina is the presence of symptoms only with exertion. Chest pain, or its angina equivillant, is a defining feature, and it is relieved by rest or nitroglycerin when exercise is ceased. Often, this is one of the first symptoms or warning indications of underlying coronary disease. The proinflammatory state is believed to aggravate endothelial dysfunction by activating endothelial cells, releases cytokines and chemokines, which may be crucial in the atherosclerotic plaque formation which is the primary cause of angina pectoris. We studied the value of some cytokines and cardiac markers as a diagnostic and follow up markers in patients with stable angina. Methodology: The study was carried out on 50 patients diagnosed with stable angina pectoris, who visited Nasiriya Heart Center throughout the period from December 2021 to April 2022,  and 20 age matched healthy person.  IL-1β, IL-5, IL-6, IL-8 were assayed at baseline (hospital admission), 1 day and 1 week post-PCI  by an enzyme-linked immunosorbent assay (ELISA).&nbsp

Structural and Mechanical Modification Induced by Water Content in Giant Wild Reed (A. donax L.)

Giant wild reed Arundo donax L. is an aggressive agricultural species with remarkable features such as fast-growing, untapped economic potential, eco-friendliness, and high specific properties (e.g., high strength/weight and modulus/weight ratios). Here, the bending properties of giant reed were studied at a molecular level to evaluate the effect of the conditions used during the preparation for their applications (also referred to as treatment conditions). The aim was to achieve new information potentially useful to suggest new possible applications of A. donax L. for structural applications in modern buildings. In this study, green reeds collected in a Sicilian plantation were dried for 2000 h in a climatic chamber under humidity-temperature-controlled conditions, then fully dried in an oven and finally re-moistened through two different procedures. The combination of different analytical techniques (such as calorimetry and fast field cycling NMR relaxometry) revealed that giant reed bending properties are strongly affected by the presence of bound water. In particular, it has been evidenced that a progressive enhancement of bending characteristics is obtained when the interactions between residual water and A. donax L. fibers become progressively stronger. For this reason, it can be suggested that fibers having different plasticity can be produced by modulate the heating treatment to regulate the amount of bound water inside A. donax L. fibers