Metabolic, Endocrine, and Immune Consequences of Sleep Deprivation

Over the last three to four decades, it has been observed that the average total hours of sleep have decreased to less than seven hours per person per night. Concomitantly, global figures relating to obesity and diabetes mellitus have increased in an alarming fashion in adults and children, and it has been hypothesized that neuro-hormonal changes accompanying this behavioral sleep deprivation may lead to insulin resistance and, subsequently, to diabetes mellitus. Sleep deprivation has been associated with multiple physiological changes, including increased cortisol and ghrelin levels, decreased leptin levels and impaired glucose metabolism. Experimental studies have also shown an increase in inflammatory and pro-inflammatory markers, which are indicators of body stress, under sleep deprivation. This review elaborates further on this hypothesis, exploring the molecular basis for the link between both entities and the underlying pathophysiology that results in insulin resistance and diabetes mellitus. We review the results of experimental and epidemiological studies, specifically examining the relationship between sleep duration and the immune and endocrine systems

Link Between Obesity and The Severity of COVID-19 Infection: A Multicenter Retrospective Study in Dubai

Background: Obesity is a major contributing factor for poor prognosis in many diseases and COVID-19 is no exception. Studies regarding the relationship between obesity and poor COVID-19 disease severity and outcome are however lacking in the gulf region. This study aimed to examine links between BMI and high mortality rate, duration of intensive care unit admission, and time on mechanical ventilation support among COVID-19 patients under the care of Dubai Health Authority (DHA) in the United Arab Emirates.Subjects dan Method: This was a retrospective, descriptive, record-based study of 637 patients admitted with confirmed SARS-CoV-2 PCR at three tertiary hospitals in Dubai, UAE. Demographic and clinical data were obtained from March 1st, 2020, through June 1st, 2020, all patients aged 18 and above were included, pregnant ladies were excluded. The dependent variables were ICU admission, COVID severity, need of respiratory support, viral clearance. The independent variables were the disease outcome between the obese and non-obese. The data were analyzed using chi-square test.Results: Total 200 (31.4%) were obese, while 435 (68.3%) were not obese. Most patients (81%) were male patients. Data Analysis reveals that obesity is associated with the risk of ICU admissions (OR=2.88, 95%CI=1.9 to 4.37; p<0.001). The findings also indicate that Covid-19 obese patients required higher respiratory support devices compared to non-obese patients (35.8% vs 16.3%, respectively) (OR= 2.87, 95%CI=1.93 to 4.27; p<0.001). Clinical severity at day 7th of hospital admissions among obese patients was direr compared to non-obese patients (34.5% vs 15.9%, respectively) (OR=2.79, 95%CI=1.87 to 4.16; p<0.001). Mortality rate at day 14 of admission were found higher among obese group too (9.5% vs 3.4%) (OR=2.95, 95%CI=1.46to 5.94; p= 0.020).Conclusion: This study indicates that COVID-19 patients with obesity (BMI more than 30 kg/m2) are found to have severer respiratory manifestations, higher mortality rate, prolonged periods of intensive care unit admission, and utilization of invasive mechanical ventilation

Comprehensive risk assessment and control measures in the food service chain of hospitals nutrition department: a case study in Al-Ahsa Governorate, Kingdom of Saudi Arabia

IntroductionEnsuring hospital food safety is essential for patient health, infection control, and public trust. This study evaluates food exposure risks in two hospitals in Al-Ahsa Governorate, Saudi Arabia, focusing on critical control points during food processing and delivery.MethodsMicrobial analysis was conducted on food samples from various stages of food preparation, including raw meat, chicken, fish, and prepared meals. Temperature monitoring and chemical hazard assessments were also carried out, including pesticide residue and heavy metal analysis.ResultsThe microbial analysis detected specific pathogens, including Salmonella, Listeria monocytogenes, Escherichia coli, and Staphylococcus aureus, along with yeasts and molds. Total bacterial counts (TBC) in raw meat, chicken, and fish ranged from 2.5 to 5.0 log cfu/g, while prepared meals had TBCs between 1.0 and 3.0 log cfu/g. No Salmonella or Listeria monocytogenes were detected. Chemical hazards, including mycotoxins in white flour and heavy metals, were within permissible limits. However, 12 pesticides were detected, with six exceeding European Food Safety Organization limits.DiscussionTemperature monitoring revealed that hot foods cooled to unsafe levels during transport, and cold samples were not consistently maintained at SFDA-recommended temperatures. Hospitals generally comply with health regulations but improvements are necessary in temperature control and preventing chemical contamination of raw materials

Multiple early introductions of SARS-CoV-2 into a global travel hub in the Middle East

International travel played a significant role in the early global spread of SARS-CoV-2. Understanding transmission patterns from different regions of the world will further inform global dynamics of the pandemic. Using data from Dubai in the United Arab Emirates (UAE), a major international travel hub in the Middle East, we establish SARS-CoV-2 full genome sequences from the index and early COVID-19 patients in the UAE. The genome sequences are analysed in the context of virus introductions, chain of transmissions, and possible links to earlier strains from other regions of the world. Phylogenetic analysis showed multiple spatiotemporal introductions of SARS-CoV-2 into the UAE from Asia, Europe, and the Middle East during the early phase of the pandemic. We also provide evidence for early community-based transmission and catalogue new mutations in SARS-CoV-2 strains in the UAE. Our findings contribute to the understanding of the global transmission network of SARS-CoV-2

SAE BAJA 2015

The SAE Baja competition is a student design competition that test a students ability to design, engineer, build, test, promote, and compete with a vehicle within the limits set by the rules. The goal is to create a single passenger off road vehicle while maintaining leading performance in speed, handling, ride, and ruggedness

Investigating Touch DNA Success Rates in Vehicle Sites for Hit-and-Run Casework

This study evaluated the effectiveness of Touch DNA recovery from four key vehicle contact points—steering wheel (SW), gear shift (GS), interior door handle (IDH), and exterior door handle (EDH)—in the context of hit-and-run forensic casework. 1769 samples were collected from 359 vehicles processed between 2020 and 2023. Statistically significant differences were observed in the quantity and quality of DNA recovered across these sites (p < 0.05). The steering wheel yielded the highest DNA success rates, followed by the gear shift, whereas the exterior and interior door handles demonstrated substantially lower recovery efficiency. These findings underscore the critical role of strategic sampling site selection in maximizing evidentiary outcomes. The results support prioritizing the steering wheel and gear shift as primary targets for DNA collection in vehicle-based investigations. The study highlights the practical utility of Touch DNA in linking individuals to vehicular crimes and calls for further research into alternative sampling techniques and contamination control measures to optimize forensic DNA recovery protocols in real-world hit-and-run scenarios

Sleep, obesity and cardiometabolic disease in children and adolescents

© 2019 Elsevier Inc. All rights reserved. Obesity and type 2 diabetes mellitus was previously limited to adults, but in recent decades, there has been an increased prevalence among children and adolescents. Given the cost burden and a plethora of adverse consequences with which these diseases are associated, obesity and cardiometabolic diseases now pose a major public health challenge. Obesity and type 2 diabetes mellitus are chronic conditions that commonly track into adulthood and also increase the likelihood of cardiovascular consequences. While these diseases can be caused by genetics, they are largely driven by lifestyle behaviors. Attempts at addressing the global epidemic have targeted behavior modification such as increasing physical activity levels and controlling dietary intake in the hope of restoring energy balance. Sleep impinges on both side of the energy balance equation and there is now an abundance of evidence to suggest that multiple features of sleep may be contributing to the onset and progression of these chronic conditions, which are discussed in this chapter. In particular, we discuss the literature pertaining to the relationship between sleep and obesity as well as type 2 diabetes mellitus in children and adolescents, while also drawing upon crucial information from adult studies. We also highlight potential mechanisms and make recommendations for future approaches which may enhance the effectiveness of interventions targeting the global epidemic of childhood obesity, which is the main driver of metabolic and cardiovascular diseases

Trace DNA Recovery: Insights from Dubai Police Casework

Trace DNA represents a critical form of forensic evidence, frequently recovered from a wide variety of touched or used items. Despite its evidentiary value, trace DNA analysis poses significant challenges due to the minute quantities of DNA involved, as well as the influence of factors such as surface type, collection methods, and environmental exposure. This study systematically examines the success rates and characteristics of trace DNA profiles recovered from six-item categories—tools, stolen items, wearable items, packaging materials, vehicles, and touched items—processed between 2021 and 2023 by the Biology and DNA Section of the Dubai Police Force. A total of 6277 cases were analyzed, encompassing a range of crimes, including homicide, suicide, missing persons, paternity disputes, and burglary. The results demonstrated an overall trace DNA success rate of 64%, with wearable items yielding the highest success rate at 76% and packaging materials yielding the lowest at 54%. Detailed analysis of positive DNA trace samples revealed significant variability in DNA profile types across item categories. Wearable items and touched items predominantly yielded full single (FS) DNA profiles, reflecting their reliability as sources of singular and high-quality DNA. Conversely, stolen items and packaging materials showed a greater prevalence of full mixed (FM) DNA profiles, highlighting their association with complex mixtures due to handling by multiple contributors. Tools and vehicles, meanwhile, exhibited higher rates of partial profiles, presenting unique challenges related to surface irregularities and environmental factors. This study emphasizes the importance of tailoring forensic strategies to item-specific characteristics, as well as the need for systematic mechanisms to categorize trace samples. Addressing operational challenges such as manual sorting and leveraging automation or AI-based systems can further streamline trace DNA analysis. The findings also underscore the importance of data sharing and standardization across forensic laboratories to enhance trace DNA recovery protocols and improve reliability in forensic investigations. Future research should focus on the effects of material properties, environmental exposure, and collection techniques on DNA retention, advancing the field of trace DNA profiling and its applications in forensic science

The Bidirectional Relationship Between Sleep and Inflammation Links Traumatic Brain Injury and Alzheimer's Disease

Traumatic brain injury (TBI) and Alzheimer’s disease (AD) are diseases during which the fine-tuned autoregulation of the brain is lost. Despite the stark contrast in their causal mechanisms, both TBI and AD are conditions which elicit a neuroinflammatory response that is coupled with physical, cognitive, and affective symptoms. One commonly reported symptom in both TBI and AD patients is disturbed sleep. Sleep is regulated by circadian and homeostatic processes such that pathological inflammation may disrupt the chemical signaling required to maintain a healthy sleep profile. In this way, immune system activation can influence sleep physiology. Conversely, sleep disturbances can exacerbate symptoms or increase the risk of inflammatory/neurodegenerative diseases. Both TBI and AD are worsened by a chronic pro-inflammatory microenvironment which exacerbates symptoms and worsens clinical outcome. Herein, a positive feedback loop of chronic inflammation and sleep disturbances is initiated. In thisreview, the bidirectional relationship between sleep disturbances and inflammation is discussed, where chronic inflammation associated with TBI and AD can lead to sleep disturbances and exacerbated neuropathology. The role of microglia and cytokines in sleep disturbances associated with these diseases is highlighted. The proposed sleep and inflammation-mediated link between TBI and AD presents an opportunity for a multifaceted approach to clinical intervention

High resolution seismic stratigraphic analysis: An integrated approach to the subsurface geology of the SE Persian Gulf

Papers included in this dissertation: Paper 1: Farzadi, P. 2006a. The development of Middle Cretaceous carbonate platforms, Persian Gulf, Iran: Constraints from seismic stratigraphy, well and biostratigraphy. Petroleum Geoscience, 12, 59-68. Paper 2: Farzadi, P. 2006b. Seismic facies analysis based on 3D multi-attribute volume classification, Dariyan Formation, SE Persian Gulf. Journal of Petroleum Geology,29/2, 159-174. Paper 3: Farzadi, P. & Hesthammer, J. (Submitted 2006). Diagnosis of the Upper Cretaceous paleokarst and turbidite systems from the Iranian Persian Gulf using volume-based multiple seismic attribute analysis and pattern recognition. N.B.: Originally accepted for publication in the AAPG Bulletin, later rejected because the US government prohibits the publication of papers using Iranian government datasets. The manuscript has been re-submitted to Petroleum Geoscience. Paper 4: Farzadi, P. & Alaei, B. (Submitted 2006). Stratigraphic architecture of the Zagros Basin: towards an objective comparison of the Fold-Thrust Belt and Foreland provinces. Submitted to the Journal of Petroleum Geology; under consideration for a thematic issue. Presentation (at international meeting and on web): Farzadi, P. 2005. Stratal geometries of the Cretaceous carbonate systems: application of multiple volumes attributes analysis to 3-D seismic data from the Persian Gulf. At: Middle to Far East Carbonate Reservoirs: Exploration, Development and Exploitation. PESGB Carbonate conference, 15th & 16th Nov. 2005 London