Estimates of non-genetic effects for measures of hunting performance in short-haired and rough-haired Italian hound

The aim of this work was to estimate the effects of eight non-genetic factors (sex, type of coat, pigmentation, type of the trial, the number of the judges in the jury, the location where the competition took place, the subjectivity of the judge and the breeders) on the assessment of seven hunting traits (morphology, the breed style, search, approach, find, pursuit and voice) for the short-haired and rough-haired Italian Hound. The data consisted of 3172 field trial records between the years 2016 and 2017. The Mann–Whitney test and Kruskal–Wallis test showed that each factor was statistically significant for some traits: the sex of the dogs was statistically significant only for the evaluation of the morphology the type of coat for breed style, search, approach, find and voice; the pigmentation for all traits excluding approach; the number of judges for morphology, approach and find; the type of trial for all traits excluding voice. Spearman's ρ (rho) correlation showed that high phenotypic correlations were between morphology, breed style and search. These traits showed low to moderate correlations with the other traits except breed style and search vs. pursuit. Furthermore, principal component analysis for the factor judge, location and breeders showed that among the seven traits assessed during the trials, the morphology and the breed style had the highest loading on the final score. Our results suggest that the effect of all the non-genetic factors analysed must be taken into account by the judges during the evaluation of the dogs.Highlights We evaluated the effects of eight non-genetic factors on the assessment of seven hunting traits for the Italian Hound. All the non-genetic factors had influence on some of the hunting traits considered. Principal component analysis showed that the highest loading for morphology and breed style are on location, judge and breeder. High phenotypic correlations were between morphology, breed style, search and pursuit

inheritance of suri and huacaya type of fleece in alpaca

The inheritance of the two types of fleece, Suri and Huacaya, observed in Alpaca (Lama pacos L.) is still not clearly defined. The objective of this work is to investigate the patter of inheritance of these two phenotypes, throughout 588 Suri x Suri and 2126 Huacaya x Huacaya offspring. The single gene and the three two-phenotype epistatic models were tested in the 19 Suri x Suri segregating families. The single dominant gene hypothesis best fitted our segregation data and could be, therefore, accepted (GT=20.276, P=0.378). The gene frequency of the recessive Huacaya allele was 0.295, being the frequency of the dominant Suri allele 0.705. The frequency of heterozygotes, estimated in the whole population and among dominant individuals, was 0.416 and 0.455, respectively, with a "carrier" Suri to Huacaya ratio of 4.780. In three Huacaya families, 3 Suri were born, as a result of a new dominant mutation on some germinal lines of Huacaya animals. The direct mutation rate can be estimated at 0.0014

Quantitative variation of melanins in alpaca (Lama pacos L.)

The amount of melanin pigments was investigated in 95 Peruvian alpaca, representative of six different fleece colours, by means of spectrophotometric assays: SpEM (Spectrophoto­metric Eumelanin), SpPM (Spectrophotometric Pheomelanin), SpASM (Spectrophotometric Alkali Soluble Melanin), and SpTM (Spectro­photometric Total Melanin). It was found that these melanin pigments were suitable for identifying three homogeneous groups, each consisting of two closely related colours. A low, an intermediate, and a high amount of SpASM, SpTM, and SpPM characterize pinkish grey and light reddish brown, brown and reddish brown, dark reddish brown and black fleeces, respectively. SpEM and SpTM provide a further split within this latter group; higher concentrations of these pigments distinguish black fleece from dark reddish brown

Bioplastic leachates characterization and impacts on early larval stages and adult mussel cellular, biochemical and physiological responses

Bioplastics are promoted as safer alternatives to tackle the long-term persistence of conventional plastics. However, information on the potential release of additives and non-intentionally added substances (NIAS) in the surrounding environment is limited, and biological effects of the leachates have been little studied. Leachates produced from three bioplastics, i.e. compostable bags (CB), bio-polyethylene terephthalate bottles (bioPET) and polylactic acid cups (PLA), and a control polymeric material, i.e. rubber tire (TR), were examined. The chemical nature of bioplastic polyesters PET, PLA and poly (butylene adipate-co-terephthalate) (PBAT) in CB, was confirmed by analytical pyrolysis. Fragments were incubated in artificial sea water for 14 days at 20 degrees C in darkness and leachate contents examined by GC-MS and HPLC-MS/MS. Catalysts and stabilizers represented the majority of chemicals in TR, while NIAS (e.g. 1,6-dioxacyclododecane-7,12-dione) were the main components of CB. Bisphenol A occurred in all leachates at a concentration range 0.3-4.8 mu g/L. Trace metals at concentrations higher than control water were found in all leachates, albeit more represented in leachates from CB and TR. A dose response to 11 dilutions of leachates (in the range 0.6-100%) was tested for biological effects on early embryo stages of Mytilus galloprovincialis. Embryotoxicity was observed in the whole range of tested concen-trations, the magnitude of effect depending on the polymers. The highest concentrations caused reduction of egg fertilization (CB, bioPET, TR) and of larvae motility (CB, PLA, TR). TR leachates also provoked larvae mortality in the range 10-100%. Effects on adult mussel physiology were evaluated after a 7-day in vivo exposure to the different leachates at 0.6% concentration. Nine biomarkers concerning lysosomal functionality, neurotransmis-sion, antioxidant and immune responses were assessed. All lysosomal parameters were affected, and serum lysozyme activity inhibited. Harmonized chemical and biological approaches are recommended to assess bio-plastic safety and support production of sustainable bioplastics

Selection program in South American domestic camelids

The document presents the genetic selection experience that is being conducted in the alpaca population of the province of Caylloma in Peru (PROMEGE, genetic improvement program) through a cooperative effort between the Center for Development Studies and Promotion (DESCO ) - Peru, the department of environmental science of the University of Camerino - Italy, the Center for new technology, energy and the environment ENEA - Italy and the SUPPRAD program of the Catholic University of Córdoba - Argentina. The program uses an open nucleus selection scheme, characterized by a nucleus managed by DESCO - CEDAT, 20 seedlings (approximately 850 animals) and more than 200 breeders (peasant families) (approximately 15,000 animals). The total population of the Caylloma province is estimated at approximately 200,000 animals, 90% Huacaya and 10% Suri, with 60% white animals and 40% colored animals. At this time the program is fully operational. PROMEGE is the first genetic selection program so far made to operate in the populations of alpacas and llamas raised in the Andean highlands. The only other example in this regard is indeed the AGE program, which operates in Australia and New Zealand, and ITALPACA, which has been operating since 2004 in Italy. The existing breeds in the alpaca are essential of the primary type, as a result of the first distribution of the genetic pool subsequent to domestication and in the domestic camelids some effects of genetic drift caused by the shock of the conquest, the primary breeds are classically characterized by a high Phenotypic variability is both of the qualitative characters (color, type of fleece) and of the quantitative characters (biometric parameters, production). For this reason, the selection approach requires particularly impressive options in the same population

Variability of fibre quality on Chinese Alashan Left Banner White Cashmere goat

The heritability and the phenotypic and genetic correlations of down weight (DW), down fibre diameter (DFD), and coefficient of variation of the down fibre diameter (CVDFD) of Chinese Alashan Left Banner White Cashmere goat were estimated on 1375 one-year-old animals, born in 2009, 2011 and 2013 and bred at the Station for Livestock Improvement of Alashan (Left Banner, Inner Mongolia, P.R. China). For all traits, significant effects were for sex, cohort and sex–cohort interaction (p < .001). The heritability for DFD and CVDFD was high, 0.41 ± 0.08 and 0.52 ± 0.06, respectively. Heritability for the DW was low (0.12 ± 0.03). Phenotypic correlation calculated by Pearson's coefficient showed that DFD is positively correlated with both CVDFD (0.29 ± 0.07) and DW (0.20 ± 0.05). The phenotypic correlation between CVDFD and DW was negative (−0.11 ± 0.06). The genetic correlations between DW and CVDFD and between DFD and CVDFD were both high and positive (0.63 ± 0.16 and 0.39 ± 0.1, respectively) while the DW showed a negative genetic correlation with DFD (−0.27 ± 0.2). Our results suggest that the selection for reducing DFD and its CVDFD is possible and a genetic progress can be achieved quickly in the Chinese Alashan Left Banner White Cashmere goat

Efecto del doble corte en la calidad del vellón y en la actividad folicular en Alpacas (Llama pacos)

La presente investigación se realizó para analizar el efecto de una doble cizalladura sobre la calidad del vellón y sobre la actividad folicular de las alpacas (Llama pacos). Se han recogido biopsias de piel y muestras de fibra de 20 alpacas (10 hembras y 10 machos) divididas en dos grupos: el grupo A, sometido a doble esquila, uno a los 9 meses y el segundo, antes de la matanza, tiempo (16 meses de edad) y grupo B, sometidos tradicionalmente a una sola esquila a los 16 meses de edad. Todos los animales fueron analizados para: i) el diámetro promedio de la fibra total ii) el diámetro promedio de la fibra primaria (medulada), iii) el diámetro promedio de la fibra medulada continua, iv) el diámetro promedio de la fibra medulada fragmentada, v) el diámetro promedio de fibra no medulada (fibra secundaria), vi) el número de folículos primarios, vii) el número de folículos secundarios, viii) el porcentaje de folículos secundarios activos, y ix) la proporción de folículos secundarios a primarios. Tanto los animales de cizalla simple como los de cizalla doble no presentaron diferencias en la calidad de la fibra y en la estructura folicular. De forma diferente, el crecimiento de la fibra mostró una diferencia altamente significativa entre los dos grupos, ya que fue más lenta en el animal compartido. Se concluyó que los "alpaqueros" pueden maximizar la producción de vellón de su alpaca joven a través del método de la doble esquila. Palabras claves: Alpaca, Folículo, Fibra, Esquila

Alpha-synuclein gene duplication: Marked intrafamilial variability in two novel pedigrees

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Fleece variation in alpaca (Vicugna pacos): a two-locus model for the Suri/Huacaya phenotype

Background: Genetic improvement of fibre-producing animal species has often induced transition from double coated to single coated fleece, accompanied by dramatic changes in skin follicles and hair composition, likely implying variation at multiple loci. Huacaya, the more common fleece phenotype in alpaca (Vicugna pacos), is characterized by a thick dense coat growing perpendicularly from the body, whereas the alternative rare and more prized single-coated Suri phenotype is distinguished by long silky fibre that grows parallel to the body and hangs in separate, distinctive pencil locks. A single-locus genetic model has been proposed for the Suri-Huacaya phenotype, where Huacaya is recessive. Results: Two reciprocal experimental test-crosses (Suri x Huacaya) were carried out, involving a total of 17 unrelated males and 149 unrelated females. An additional dataset of 587 offspring of Suri x Suri crosses was analyzed. Segregation ratios, population genotype frequencies, and/or recombination fraction under different genetic models were estimated by maximum likelihood. The single locus model for the Suri/Huacaya phenotype was rejected. In addition, we present two unexpected observations: 1) a large proportion (about 3/4) of the Suri animals are segregating (with at least one Huacaya offspring), even in breeding conditions where the Huacaya trait would have been almost eliminated; 2) a model with two different values of the segregation ratio fit the data significantly better than a model with a single parameter. Conclusions: The data support a genetic model in which two linked loci must simultaneously be homozygous for recessive alleles in order to produce the Huacaya phenotype. The estimated recombination rate between these loci was 0.099 (95% C. L. = 0.029-0.204). Our genetic analysis may be useful for other species whose breeding system produces mainly half-sib families