What does it take to detect a change in soil carbon stock? A regional comparison of minimum detectable difference and experiment duration in the north central United States

Variability in soil organic carbon (SOC) results from natural and human processes interacting across time and space, and leads to large variation in the minimum difference in SOC that can be detected with a particular experimental design. Here we report a unique comparison of minimum detectable differences (MDDs) in SOC, and the estimated times required to observe those MDDs across the north central United States, calculated for the two most common SOC experiments: (1) a comparison between two treatments, e.g., moldboard plow (MP) and no-tillage (NT), using a randomized complete block design experiment; and (2) a comparison of changes in SOC over time for a particular treatment, e.g., NT, using a randomized complete block design experiment with time as an additional factor. We estimated the duration of the two experiment types required to achieve MDD through simulation of SOC dynamics. Data for the study came from 13 experimental sites located in Iowa, Illinois, Ohio, Michigan, Wisconsin, Missouri, and Minnesota. Soil organic carbon, bulk density, and texture were measured at four soil depths. Minimum detectable differences were calculated with probability of Type I error of 0.05 and probability of Type II error of 0.15. The MDDs in SOC were highly variable across the region and increased with soil depth. At 0 to 10 cm (0 to 3.9 in) soil depth, MDDs with five replications ranged from 1.04 g C kg−1 (0.017 oz C lb−1; 6%) to 7.15 g C kg−1 (0.114 oz C lb−1; 31%) for comparison of two treatments; and from 0.46 g C kg−1 (0.007 oz C lb−1; 3%) to 3.12 g C kg−1 (0.050 oz C lb−1; 13%) for SOC change over time. Large differences were also predicted in the experiment duration required to detect a difference in SOC between MP and NT (from 8 to \u3e100 years with five replications), or a change in SOC over time under NT management (from 11 to 71 years with five replications). At most locations, the time required to detect a change in SOC under NT was shorter than the time required to detect a difference between MP and NT. Minimum detectable difference and experiment duration decreased with the number of replications and were correlated with SOC variability and soil texture of the experimental sites, i.e., they tended to be lower in fine textured soils. Experiment duration was also reduced by increased crop productivity and the amount of residue left on the soil. The relationships and methods described here enable the design of experiments with high power of detecting differences and changes in SOC and enhance our understanding of how management practices influence SOC storage

U.S. cereal rye winter cover crop growth database

Winter cover crop performance metrics (i.e., vegetative biomass quantity and quality) affect ecosystem services provisions, but they vary widely due to differences in agronomic practices, soil properties, and climate. Cereal rye (Secale cereale) is the most common winter cover crop in the United States due to its winter hardiness, low seed cost, and high biomass production. We compiled data on cereal rye winter cover crop performance metrics, agronomic practices, and soil properties across the eastern half of the United States. The dataset includes a total of 5,695 cereal rye biomass observations across 208 site-years between 2001–2022 and encompasses a wide range of agronomic, soils, and climate conditions. Cereal rye biomass values had a mean of 3,428 kg ha−1, a median of 2,458 kg ha−1, and a standard deviation of 3,163 kg ha−1. The data can be used for empirical analyses, to calibrate, validate, and evaluate process-based models, and to develop decision support tools for management and policy decisions.This article is published as Huddell, A.M., Thapa, R., Marcillo, G.S. et al. U.S. cereal rye winter cover crop growth database. Sci Data 11, 200 (2024). https://doi.org/10.1038/s41597-024-02996-9. Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted

Optimizing care in osteoporosis: The Canadian quality circle project

<p>Abstract</p> <p>Background</p> <p>While the Osteoporosis Canada 2002 Canadian guidelines provided evidence based strategies in preventing, diagnosing, and managing this condition, publication and distribution of guidelines have not, in and of themselves, been shown to alter physicians clinical approaches. We hypothesize that primary care physicians enrolled in the Quality Circle project would change their patient management of osteoporosis in terms of awareness of osteoporosis risk factors and bone mineral density testing in accordance with the guidelines.</p> <p>Methods</p> <p>The project consisted of five Quality Circle phases that included: 1) Training & Baseline Data Collection, 2) First Educational Intervention & First Follow-Up Data Collection 3) First Strategy Implementation Session, 4) Final Educational Intervention & Final Follow-up Data Collection, and 5) Final Strategy Implementation Session. A total of 340 circle members formed 34 quality circles and participated in the study. The generalized estimating equations approach was used to model physician awareness of risk factors for osteoporosis and appropriate utilization of bone mineral density testing pre and post educational intervention (first year of the study). Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated.</p> <p>Results</p> <p>After the 1^styear of the study, physicians' certainty of their patients' risk factor status increased. Certainty varied from an OR of 1.4 (95% CI: 1.1, 1.8) for prior vertebral fracture status to 6.3 (95% CI: 2.3, 17.9) for prior hip fracture status. Furthermore, bone mineral density testing increased in high risk as compared with low risk patients (OR: 1.4; 95% CI: 1.2, 1.7).</p> <p>Conclusion</p> <p>Quality Circle methodology was successful in increasing both physicians' awareness of osteoporosis risk factors and appropriate bone mineral density testing in accordance with the 2002 Canadian guidelines.</p

bZIP-Type transcription factors CREB and OASIS bind and stimulate the promoter of the mammalian transcription factor GCMa/Gcm1 in trophoblast cells

One of the master regulators of placental cell fusion in mammals leading to multi-nucleated syncytiotrophoblasts is the transcription factor GCMa. Recently, we proved that the cAMP-driven protein kinase A signaling pathway is fundamental for up-regulation of GCMa transcript levels and protein stability. Here, we show that Transducer of Regulated CREB activity (TORC1), the human co-activator of cAMP response element-binding protein (CREB), but not a dominant-negative CREB mutant, significantly up-regulates the GCMa promoter. We identified potential cAMP response element (CRE)-binding sites within the GCMa promoter upstream of the transcriptional start site. Only the CRE site at -1337 interacted strongly with CREB in promoter mapping experiments. The characterization of GCMa promoter mutants and additional bZIP-type family members demonstrated that also old astrocyte specifically-induced substance (OASIS) is able to stimulate GCMa transcription. Knockdown of endogenous CREB or OASIS in BeWo cells decreased endogenous GCMa mRNA level and activity. Overexpression of TORC1 or OASIS in choriocarcinoma cells led to placental cell fusion, accompanied by placental expression of gap junction forming protein connexin-43. Further, we show that CREB expression is replaced by OASIS expression around E12.5 suggesting that a sequential order of bZIP-type family members ensures a high rate of GCMa transcription throughout placentation

U.S. cereal rye winter cover crop growth database

Winter cover crop performance metrics (i.e., vegetative biomass quantity and quality) affect ecosystem services provisions, but they vary widely due to differences in agronomic practices, soil properties, and climate. Cereal rye (Secale cereale) is the most common winter cover crop in the United States due to its winter hardiness, low seed cost, and high biomass production. We compiled data on cereal rye winter cover crop performance metrics, agronomic practices, and soil properties across the eastern half of the United States. The dataset includes a total of 5,695 cereal rye biomass observations across 208 site-years between 2001–2022 and encompasses a wide range of agronomic, soils, and climate conditions. Cereal rye biomass values had a mean of 3,428 kg ha−1, a median of 2,458 kg ha−1, and a standard deviation of 3,163 kg ha−1. The data can be used for empirical analyses, to calibrate, validate, and evaluate process-based models, and to develop decision support tools for management and policy decisions

Tarifliches Wahlrecht: Warum die Mehrheit der Beschäftigten lieber mehr Zeit hätte als mehr Geld

Ruf K, Bächmann A-C, Abendroth A, Mellies A. Tarifliches Wahlrecht: Warum die Mehrheit der Beschäftigten lieber mehr Zeit hätte als mehr Geld. IAB Forum. 2024.Einige Tarifverträge sehen mittlerweile für bestimmte Beschäftigtengruppen eine Wahlmöglichkeit zwischen „mehr Zeit“ oder „mehr Geld“ vor. Eine Befragung von über 3.000 Beschäftigten aus über 150 betroffenen Betrieben zeigt, dass die meisten Beschäftigten unter diesen Umständen bereit sind, zugunsten von mehr Freizeit auf Lohnerhöhungen oder Sonderzahlungen zu verzichten

Soziale Strukturierungen der tariflichen Wahlmöglichkeit zwischen Zeit und Geld

Mellies A, Abendroth A, Bächmann A-C, Ruf K. Die Qual der Wahl? Soziale Strukturierungen der tariflichen Wahlmöglichkeit zwischen Zeit und Geld. WSI-Mitteilungen. 2025;1/2025(1):22-29.Einige Gewerkschaften in Deutschland haben zuletzt ein flexibles Arbeitsarrangement durchgesetzt, bei dem sich die Beschäftigten jährlich neu zwischen mehr Zeit oder mehr Geld entscheiden können. Diese Wahloption bietet damit das Potenzial, die persönliche Arbeitszeit flexibler an unterschiedliche Lebensphasen anzupassen. Der Beitrag analysiert anhand eines verknüpften Betriebs- und Beschäftigtendatensatzes, inwiefern sich Männer und Frauen mit und ohne Kinder unter 14 Jahren im Haushalt bei der Wahl von Zeit sowie bei den dahinterliegenden Motiven ihrer Wahl unterscheiden. Die Ergebnisse zeigen zum einen, dass Frauen häufiger Zeit wählen als Männer. Zum anderen geben Mütter und Väter die Zeit für Hobbies, Freunde und sich selbst deutlich häufiger als wichtigsten Grund für die Zeitwahl an als Frauen und Männer ohne Kinder unter 14 Jahren im Haushalt. Die Ergebnisse sprechen somit dafür, dass sich auch im Kontext der Wahloption geschlechts- und elternschaftsspezifische Unterschiede finden lassen

Varicella Zoster Virus infects mucosal associated Invariant T cells

IntroductionMucosal Associated Invariant T (MAIT) cells are innate-like T cells that respond to conserved pathogen-derived vitamin B metabolites presented by the MHC class I related-1 molecule (MR1) antigen presentation pathway. Whilst viruses do not synthesize these metabolites, we have reported that varicella zoster virus (VZV) profoundly suppresses MR1 expression, implicating this virus in manipulation of the MR1:MAIT cell axis. During primary infection, the lymphotropism of VZV is likely to be instrumental in hematogenous dissemination of virus to gain access to cutaneous sites where it clinically manifests as varicella (chickenpox). However, MAIT cells, which are found in the blood and at mucosal and other organ sites, have yet to be examined in the context of VZV infection. The goal of this study was to examine any direct impact of VZV on MAIT cells.MethodsUsing flow cytometry, we interrogated whether primary blood derived MAIT cells are permissive to infection by VZV whilst further analysing differential levels of infection between various MAIT cell subpopulations. Changes in cell surface extravasation, skin homing, activation and proliferation markers after VZV infection of MAIT cells was also assessed via flow cytometry. Finally the capacity of MAIT cells to transfer infectious virus was tested through an infectious center assay and imaged via fluorescence microscopy.ResultsWe identify primary blood-derived MAIT cells as being permissive to VZV infection. A consequence of VZV infection of MAIT cells was their capacity to transfer infectious virus to other permissive cells, consistent with MAIT cells supporting productive infection. When subgrouping MAIT cells by their co- expression of a variety cell surface markers, there was a higher proportion of VZV infected MAIT cells co-expressing CD4+ and CD4+/CD8+ MAIT cells compared to the more phenotypically dominant CD8+ MAIT cells, whereas infection was not associated with differences in co-expression of CD56 (MAIT cell subset with enhanced responsiveness to innate cytokine stimulation), CD27 (co-stimulatory) or PD-1 (immune checkpoint). Infected MAIT cells retained high expression of CCR2, CCR5, CCR6, CLA and CCR4, indicating a potentially intact capacity for transendothelial migration, extravasation and trafficking to skin sites. Infected MAIT cells also displayed increased expression of CD69 (early activation) and CD71 (proliferation) markers.DiscussionThese data identify MAIT cells as being permissive to VZV infection and identify impacts of such infection on co- expressed functional markers

Methodenbericht der Beschäftigtenbefragung. „Die Wahl zwischen Zeit oder Geld: neue Flexibilität für eine bessere Vereinbarkeit von Beruf und Privatem?“

Ruf K, Mellies A, Abendroth A, Müller D, Moths O. Methodenbericht der Beschäftigtenbefragung. „Die Wahl zwischen Zeit oder Geld: neue Flexibilität für eine bessere Vereinbarkeit von Beruf und Privatem?“. Bielefeld: Universität Bielefeld, Fakultät für Soziologie; 2024

Women at Work: Women's Access to Power and the Gender Earning Gap

Abendroth A, Melzer SM, Kalev A, Tomaskovic-Devey D. Women at Work: Women's Access to Power and the Gender Earning Gap. ILR Review. 2017;70(1):190-222.Using a unique sample of 5,022 workers in 94 large German work-places, the authors explore whether and how women's access to higher level positions, firms' human resources practices, and workers' qualification levels are associated with gender differences in earnings. First, they find that having more women in management reduces the gender earnings gap for jobs with low qualifications, but not those with high qualifications. Second, they find that while men's compensation is positively affected by having a male supervisor, women with a female supervisor do not receive such an advantage. Finally, they find that human resources practices and job-level qualifications moderate the association between gendered power and gender earnings inequalities. Integrating women into managerial and supervisory roles does not automatically reduce gender inequalities; its impacts are contingent on organizational context