Molecular epidemiology of contagious bovine pleuropneumonia by detection, identification and differentiation of Mycoplasma mycoides subsp. mycoides in Niger State, Nigeria

The study was aimed at epidemiologically detecting, identifying, and differentiating Mycoplasma mycoides subsp. mycoides strains according to their geographical origins in asymptomatic cattle populations of Niger State, North-central Nigeria between January and August 2013, using conventional and fingerprinting PCR assays without culturing or enriching of the field samples. Cross sectional molecular study of asymptomatic cattle sampled in the major abattoirs and livestock markets was carried out. Geographical lineage differentiation of the strains was performed on the basis of presence or absence of 8.84 kb genomic segment. With primers pair MM450/MM451, two 574 bp genomic fragments of DNA of Mycoplasma mycoides subsp. mycoides were detected. Using fingerprinting PCR; with insertion element IS1296/R(all) primers pair the detected two strains of Mycoplasma mycoides subsp. mycoides genomic DNA were specifically identified by generation of a fragment of 1.1 kb; with IS1296/R1 primers pair, identified Mycoplasma mycoides subsp. mycoides strains were differentiated to be of African cluster by generation of 1.4 kb. The PCR procedures were sensitive and produced rapid results that identified two strains of Mycoplasma mycoides subsp. mycoides of African/Australian origin. The presence of Mycoplasma mycoides subsp. mycoides African/Australian strains, which is useful for vaccine production, was therefore established in Niger State, Nigeria.Keywords: Cattle, Control, Cross-sectional molecular study, Mmm , Molecular fingerprinting, Nigeri

Global burden of bacterial antimicrobial resistance 1990–2021: a systematic analysis with forecasts to 2050

BACKGROUND: Antimicrobial resistance (AMR) poses an important global health challenge in the 21st century. A previous study has quantified the global and regional burden of AMR for 2019, followed with additional publications that provided more detailed estimates for several WHO regions by country. To date, there have been no studies that produce comprehensive estimates of AMR burden across locations that encompass historical trends and future forecasts. METHODS: We estimated all-age and age-specific deaths and disability-adjusted life-years (DALYs) attributable to and associated with bacterial AMR for 22 pathogens, 84 pathogen–drug combinations, and 11 infectious syndromes in 204 countries and territories from 1990 to 2021. We collected and used multiple cause of death data, hospital discharge data, microbiology data, literature studies, single drug resistance profiles, pharmaceutical sales, antibiotic use surveys, mortality surveillance, linkage data, outpatient and inpatient insurance claims data, and previously published data, covering 520 million individual records or isolates and 19 513 study-location-years. We used statistical modelling to produce estimates of AMR burden for all locations, including those with no data. Our approach leverages the estimation of five broad component quantities: the number of deaths involving sepsis; the proportion of infectious deaths attributable to a given infectious syndrome; the proportion of infectious syndrome deaths attributable to a given pathogen; the percentage of a given pathogen resistant to an antibiotic of interest; and the excess risk of death or duration of an infection associated with this resistance. Using these components, we estimated disease burden attributable to and associated with AMR, which we define based on two counterfactuals; respectively, an alternative scenario in which all drug-resistant infections are replaced by drug-susceptible infections, and an alternative scenario in which all drug-resistant infections were replaced by no infection. Additionally, we produced global and regional forecasts of AMR burden until 2050 for three scenarios: a reference scenario that is a probabilistic forecast of the most likely future; a Gram-negative drug scenario that assumes future drug development that targets Gram-negative pathogens; and a better care scenario that assumes future improvements in health-care quality and access to appropriate antimicrobials. We present final estimates aggregated to the global, super-regional, and regional level. FINDINGS: In 2021, we estimated 4·71 million (95% UI 4·23–5·19) deaths were associated with bacterial AMR, including 1·14 million (1·00–1·28) deaths attributable to bacterial AMR. Trends in AMR mortality over the past 31 years varied substantially by age and location. From 1990 to 2021, deaths from AMR decreased by more than 50% among children younger than 5 years yet increased by over 80% for adults 70 years and older. AMR mortality decreased for children younger than 5 years in all super-regions, whereas AMR mortality in people 5 years and older increased in all super-regions. For both deaths associated with and deaths attributable to AMR, meticillin-resistant Staphylococcus aureus increased the most globally (from 261 000 associated deaths [95% UI 150 000–372 000] and 57 200 attributable deaths [34 100–80 300] in 1990, to 550 000 associated deaths [500 000–600 000] and 130 000 attributable deaths [113 000–146 000] in 2021). Among Gram-negative bacteria, resistance to carbapenems increased more than any other antibiotic class, rising from 619 000 associated deaths (405 000–834 000) in 1990, to 1·03 million associated deaths (909 000–1·16 million) in 2021, and from 127 000 attributable deaths (82 100–171 000) in 1990, to 216 000 (168 000–264 000) attributable deaths in 2021. There was a notable decrease in non-COVID-related infectious disease in 2020 and 2021. Our forecasts show that an estimated 1·91 million (1·56–2·26) deaths attributable to AMR and 8·22 million (6·85–9·65) deaths associated with AMR could occur globally in 2050. Super-regions with the highest all-age AMR mortality rate in 2050 are forecasted to be south Asia and Latin America and the Caribbean. Increases in deaths attributable to AMR will be largest among those 70 years and older (65·9% [61·2–69·8] of all-age deaths attributable to AMR in 2050). In stark contrast to the strong increase in number of deaths due to AMR of 69·6% (51·5–89·2) from 2022 to 2050, the number of DALYs showed a much smaller increase of 9·4% (–6·9 to 29·0) to 46·5 million (37·7 to 57·3) in 2050. Under the better care scenario, across all age groups, 92·0 million deaths (82·8–102·0) could be cumulatively averted between 2025 and 2050, through better care of severe infections and improved access to antibiotics, and under the Gram-negative drug scenario, 11·1 million AMR deaths (9·08–13·2) could be averted through the development of a Gram-negative drug pipeline to prevent AMR deaths. INTERPRETATION: This study presents the first comprehensive assessment of the global burden of AMR from 1990 to 2021, with results forecasted until 2050. Evaluating changing trends in AMR mortality across time and location is necessary to understand how this important global health threat is developing and prepares us to make informed decisions regarding interventions. Our findings show the importance of infection prevention, as shown by the reduction of AMR deaths in those younger than 5 years. Simultaneously, our results underscore the concerning trend of AMR burden among those older than 70 years, alongside a rapidly ageing global community. The opposing trends in the burden of AMR deaths between younger and older individuals explains the moderate future increase in global number of DALYs versus number of deaths. Given the high variability of AMR burden by location and age, it is important that interventions combine infection prevention, vaccination, minimisation of inappropriate antibiotic use in farming and humans, and research into new antibiotics to mitigate the number of AMR deaths that are forecasted for 2050. FUNDING: UK Department of Health and Social Care's Fleming Fund using UK aid, and the Wellcome Trust

Global burden of bacterial antimicrobial resistance 1990-2021: a systematic analysis with forecasts to 2050

Background Antimicrobial resistance (AMR) poses an important global health challenge in the 21st century. A previous study has quantified the global and regional burden of AMR for 2019, followed with additional publications that provided more detailed estimates for several WHO regions by country. To date, there have been no studies that produce comprehensive estimates of AMR burden across locations that encompass historical trends and future forecasts. Methods We estimated all-age and age-specific deaths and disability-adjusted life-years (DALYs) attributable to and associated with bacterial AMR for 22 pathogens, 84 pathogen–drug combinations, and 11 infectious syndromes in 204 countries and territories from 1990 to 2021. We collected and used multiple cause of death data, hospital discharge data, microbiology data, literature studies, single drug resistance profiles, pharmaceutical sales, antibiotic use surveys, mortality surveillance, linkage data, outpatient and inpatient insurance claims data, and previously published data, covering 520 million individual records or isolates and 19 513 study-location-years. We used statistical modelling to produce estimates of AMR burden for all locations, including those with no data. Our approach leverages the estimation of five broad component quantities: the number of deaths involving sepsis; the proportion of infectious deaths attributable to a given infectious syndrome; the proportion of infectious syndrome deaths attributable to a given pathogen; the percentage of a given pathogen resistant to an antibiotic of interest; and the excess risk of death or duration of an infection associated with this resistance. Using these components, we estimated disease burden attributable to and associated with AMR, which we define based on two counterfactuals; respectively, an alternative scenario in which all drug-resistant infections are replaced by drug-susceptible infections, and an alternative scenario in which all drug-resistant infections were replaced by no infection. Additionally, we produced global and regional forecasts of AMR burden until 2050 for three scenarios: a reference scenario that is a probabilistic forecast of the most likely future; a Gram-negative drug scenario that assumes future drug development that targets Gram-negative pathogens; and a better care scenario that assumes future improvements in health-care quality and access to appropriate antimicrobials. We present final estimates aggregated to the global, super-regional, and regional level. Findings In 2021, we estimated 4·71 million (95% UI 4·23–5·19) deaths were associated with bacterial AMR, including 1·14 million (1·00–1·28) deaths attributable to bacterial AMR. Trends in AMR mortality over the past 31 years varied substantially by age and location. From 1990 to 2021, deaths from AMR decreased by more than 50% among children younger than 5 years yet increased by over 80% for adults 70 years and older. AMR mortality decreased for children younger than 5 years in all super-regions, whereas AMR mortality in people 5 years and older increased in all super-regions. For both deaths associated with and deaths attributable to AMR, meticillin-resistant Staphylococcus aureus increased the most globally (from 261 000 associated deaths [95% UI 150 000–372 000] and 57 200 attributable deaths [34 100–80 300] in 1990, to 550 000 associated deaths [500 000–600 000] and 130 000 attributable deaths [113 000–146 000] in 2021). Among Gram-negative bacteria, resistance to carbapenems increased more than any other antibiotic class, rising from 619 000 associated deaths (405 000–834 000) in 1990, to 1·03 million associated deaths (909 000–1·16 million) in 2021, and from 127 000 attributable deaths (82 100–171 000) in 1990, to 216 000 (168 000–264 000) attributable deaths in 2021. There was a notable decrease in non-COVID-related infectious disease in 2020 and 2021. Our forecasts show that an estimated 1·91 million (1·56–2·26) deaths attributable to AMR and 8·22 million (6·85–9·65) deaths associated with AMR could occur globally in 2050. Super-regions with the highest all-age AMR mortality rate in 2050 are forecasted to be south Asia and Latin America and the Caribbean. Increases in deaths attributable to AMR will be largest among those 70 years and older (65·9% [61·2–69·8] of all-age deaths attributable to AMR in 2050). In stark contrast to the strong increase in number of deaths due to AMR of 69·6% (51·5–89·2) from 2022 to 2050, the number of DALYs showed a much smaller increase of 9·4% (–6·9 to 29·0) to 46·5 million (37·7 to 57·3) in 2050. Under the better care scenario, across all age groups, 92·0 million deaths (82·8–102·0) could be cumulatively averted between 2025 and 2050, through better care of severe infections and improved access to antibiotics, and under the Gram-negative drug scenario, 11·1 million AMR deaths (9·08–13·2) could be averted through the development of a Gram-negative drug pipeline to prevent AMR deaths. Interpretation This study presents the first comprehensive assessment of the global burden of AMR from 1990 to 2021, with results forecasted until 2050. Evaluating changing trends in AMR mortality across time and location is necessary to understand how this important global health threat is developing and prepares us to make informed decisions regarding interventions. Our findings show the importance of infection prevention, as shown by the reduction of AMR deaths in those younger than 5 years. Simultaneously, our results underscore the concerning trend of AMR burden among those older than 70 years, alongside a rapidly ageing global community. The opposing trends in the burden of AMR deaths between younger and older individuals explains the moderate future increase in global number of DALYs versus number of deaths. Given the high variability of AMR burden by location and age, it is important that interventions combine infection prevention, vaccination, minimisation of inappropriate antibiotic use in farming and humans, and research into new antibiotics to mitigate the number of AMR deaths that are forecasted for 2050. Funding UK Department of Health and Social Care's Fleming Fund using UK aid, and the Wellcome Trust

Epizootiological Survey of Bovine Brucellosis in Nomadic Pastoral Camps in Niger State, Nigeria

Bovine brucellosis is a bacterial zoonotic disease of cattle caused by members of the genus Brucella. A cross sectional study was conducted in three nomadic pastoral camps to determine seroprevalence of the disease and assess herders’ exiting veterinary knowledge and traditional oral history about the disease, using Rose Bengal Plate Test (RBPT) and participatory rural appraisal (PRA) tools respectively.  Sample size of 87 was obtained for quantitative analysis while six key informants were conveniently selected for qualitative information. The within-camp seropositive varied between 1 (3.45%) in Paiko pastoral camp to 4 (13.79%) each in Bobi grazing reserve and Eyagi pastoral camps. The overall seroprevalence was 10.35%. Results of existing veterinary knowledge and traditional oral history exercises indicate that bovine brucellosis has high impact in nomadic pastoral cattle camps with strong agreements of W= 0.787, P<0.05 among the six key informants. They called bovine brucellosis (Bakkale) and described it as a cattle disease characterized by standing hair coat, fever, loss of appetite, swollen joints, and abortion and transmitted by ingestion and contact. The high prevalence observed calls for urgent government intervention towards public health enlightenment of pastoral nomads on the zoonotic nature and danger of the disease. Government and non-governmental organizations (NGOs) should initiate routine screening of pastoral nomads and their herds, especially those that are potential reservoirs and those at risk of exposure with consequent free treatment for animals and humans found positive.Keywords: Bovine brucellosis, seroprevalence, existing veterinary knowledge, pastoral camps

Factors affecting workers’ delivery of good hygienic and sanitary operations in slaughterhouses in north-central Nigeria

This survey assesses the levels of knowledge and preventive practices of workers on the hygienic and sanitary operations in slaughterhouses in Niger State, north-central Nigeria. A cross sectional survey was conducted on 385 workers aged 20 years and above between January 2013 and April 2013 using structured questionnaires and checklist to assess their knowledge and preventive practices about slaughterhouse operations. Majority  (94.0%) of the respondents were males and most (69.6%) were married. Majority (34.3%) of the workers were in the age group 30–39 years. The mean age of the workers was 40.8±10.7 years. One in five (19.2%) were illiterates. Majority of the respondents 95.6% and 96.4% for meat hygiene and sanitation respectively did not have any previous training. About two-third of the workers (74.5%) had poor knowledge about good slaughterhouse operations and more than two third (86.2%) engage in poor preventive practices. The slaughterhouse workers in the age group 60-69 years were less likely [OR 0.1345; 95% CI 0.0397, 0.4553)] to have poor knowledge of the operations than those in the 20-29 age group. The workers with secondary and tertiary education were less likely [OR 0.3557; 95% CI (0.1706, 0.7418) and OR 0.1259; 95% CI (0.0556, 0.2851) respectively] to have poor knowledge than those without formal education. Workers who know correct definition of slaughterhouse hygiene were less likely [OR 0.3125; 95% CI (0.1862, 0.5244)] to demonstrate poor preventive practices, and those who are aware of the effects of improper operations on public and environmental health were more likely [OR 6.587; 95% CI (4.094, 10.6)] to demonstrate satisfactory preventive practices. This survey indicates the need to sensitize and organize trainings for slaughterhouse workers to improve their knowledge of standard slaughterhouse operations to produce wholesome meat and safeguard public and environmental health.Keywords: Abattoir workers, knowledge, operations, preventive practices, slaughterhouse, Nigeri

Factors affecting workers’ delivery of good hygienic and sanitary operations in slaughterhouses in north-central Nigeria

This survey assesses the levels of knowledge and preventive practices of workers on the hygienic and sanitary operations in slaughterhouses in Niger State, north-central Nigeria. A cross sectional survey was conducted on 385 workers aged 20 years and above between January 2013 and April 2013 using structured questionnaires and checklist to assess their knowledge and preventive practices about slaughterhouse operations. Majority  (94.0%) of the respondents were males and most (69.6%) were married. Majority (34.3%) of the workers were in the age group 30–39 years. The mean age of the workers was 40.8±10.7 years. One in five (19.2%) were illiterates. Majority of the respondents 95.6% and 96.4% for meat hygiene and sanitation respectively did not have any previous training. About two-third of the workers (74.5%) had poor knowledge about good slaughterhouse operations and more than two third (86.2%) engage in poor preventive practices. The slaughterhouse workers in the age group 60-69 years were less likely [OR 0.1345; 95% CI 0.0397, 0.4553)] to have poor knowledge of the operations than those in the 20-29 age group. The workers with secondary and tertiary education were less likely [OR 0.3557; 95% CI (0.1706, 0.7418) and OR 0.1259; 95% CI (0.0556, 0.2851) respectively] to have poor knowledge than those without formal education. Workers who know correct definition of slaughterhouse hygiene were less likely [OR 0.3125; 95% CI (0.1862, 0.5244)] to demonstrate poor preventive practices, and those who are aware of the effects of improper operations on public and environmental health were more likely [OR 6.587; 95% CI (4.094, 10.6)] to demonstrate satisfactory preventive practices. This survey indicates the need to sensitize and organize trainings for slaughterhouse workers to improve their knowledge of standard slaughterhouse operations to produce wholesome meat and safeguard public and environmental health.Keywords: Abattoir workers, knowledge, operations, preventive practices, slaughterhouse, Nigeria</jats:p